Complementary peptides represent a credible alternative to agrochemicals by activating translation of targeted proteins

Mélanie Ormancey; Bruno Guillotin; Rémy Merret; Laurent Camborde; Carine Duboé; Bertrand Fabre; Cécile Pouzet; Francis Impens; Delphi Van Haver; Marie Christine Carpentier; Hélène San Clemente; Marielle Aguilar; Dominique Lauressergues; Lars B. Scharff; Carole Pichereaux; Odile Burlet-Schiltz; Cécile Bousquet-Antonelli; Kris Gevaert; Patrice Thuleau; Serge Plaza; Jean Philippe Combier

doi:10.1038/s41467-023-35951-0

Complementary peptides represent a credible alternative to agrochemicals by activating translation of targeted proteins

Mélanie Ormancey, Bruno Guillotin, Rémy Merret, Laurent Camborde, Carine Duboé, Bertrand Fabre, Cécile Pouzet, Francis Impens, Delphi Van Haver, Marie Christine Carpentier, Hélène San Clemente, Marielle Aguilar, Dominique Lauressergues, Lars B. Scharff, Carole Pichereaux, Odile Burlet-Schiltz, Cécile Bousquet-Antonelli, Kris Gevaert, Patrice Thuleau, Serge PlazaJean Philippe Combier^*

^*Corresponding author af dette arbejde

Copenhagen Plant Science Center

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › peer review

13 Citationer (Scopus)

8 Downloads (Pure)

Abstract

The current agriculture main challenge is to maintain food production while
facing multiple threats such as increasing world population, temperature
increase, lack of agrochemicals due to health issues and uprising of weeds
resistant to herbicides. Developing novel, alternative, and safe methods is hence
of paramount importance. Here, we show that complementary peptides (cPEPs)
fromany gene can be designed to target specifically plant coding genes. External
application of synthetic peptides increases the abundance of the targeted protein,
leading to related phenotypes. Moreover, we provide evidence that cPEPs
can be powerful tools in agronomy to improve plant traits, such as growth,
resistance to pathogen or heat stress, without the needs of genetic approaches.
Finally, by combining their activity they can also be used to reduce weed growth.

Originalsprog	Engelsk
Artikelnummer	254
Tidsskrift	Nature Communications
Vol/bind	14
Antal sider	13
ISSN	2041-1723
DOI	https://doi.org/10.1038/s41467-023-35951-0
Status	Udgivet - 2023

Bibliografisk note

Funding Information:
This work was funded by the French ANR project BiomiPEP (ANR-16-CE12-0018-01). B.F. has been supported by the Fondation ARC pour la recherche sur le cancer. The work (proteomics) was funded in part by grants from the Région Occitanie, European funds (Fonds Européens de Développement Régional, FEDER), Toulouse Métropole, and the French Ministry of Research with the Investissement d’Avenir Infrastructures Nationales en Biologie et Santé program (ProFI, Proteomics French Infrastructure project, ANR-10-INBS-08). We thank Christian Mazars (LRSV, Auzeville-Tolosane, France) for valuable advice about CPK3, Marc Knight (Durham University, UK) for Arabidopsis ABRE Luciferase lines, and Camille Ribeyre (Micropep Technologies) for Botrytis cinerea spores. This work was supported by UPVD University through the utilization of the NextSeq 550 device at the Bioenvironment Platform.

Funding Information:
This work was funded by the French ANR project BiomiPEP (ANR-16-CE12-0018-01). B.F. has been supported by the Fondation ARC pour la recherche sur le cancer. The work (proteomics) was funded in part by grants from the Région Occitanie, European funds (Fonds Européens de Développement Régional, FEDER), Toulouse Métropole, and the French Ministry of Research with the Investissement d’Avenir Infrastructures Nationales en Biologie et Santé program (ProFI, Proteomics French Infrastructure project, ANR-10-INBS-08). We thank Christian Mazars (LRSV, Auzeville-Tolosane, France) for valuable advice about CPK3, Marc Knight (Durham University, UK) for Arabidopsis ABRE Luciferase lines, and Camille Ribeyre (Micropep Technologies) for Botrytis cinerea spores. This work was supported by UPVD University through the utilization of the NextSeq 550 device at the Bioenvironment Platform.

Publisher Copyright:
© 2023, The Author(s).

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Citationsformater

Ormancey, M., Guillotin, B., Merret, R., Camborde, L., Duboé, C., Fabre, B., Pouzet, C., Impens, F., Van Haver, D., Carpentier, M. C., Clemente, H. S., Aguilar, M., Lauressergues, D., Scharff, L. B., Pichereaux, C., Burlet-Schiltz, O., Bousquet-Antonelli, C., Gevaert, K., Thuleau, P., ... Combier, J. P. (2023). Complementary peptides represent a credible alternative to agrochemicals by activating translation of targeted proteins. Nature Communications, 14, Artikel 254. https://doi.org/10.1038/s41467-023-35951-0

Ormancey, M, Guillotin, B, Merret, R, Camborde, L, Duboé, C, Fabre, B, Pouzet, C, Impens, F, Van Haver, D, Carpentier, MC, Clemente, HS, Aguilar, M, Lauressergues, D, Scharff, LB, Pichereaux, C, Burlet-Schiltz, O, Bousquet-Antonelli, C, Gevaert, K, Thuleau, P, Plaza, S & Combier, JP 2023, 'Complementary peptides represent a credible alternative to agrochemicals by activating translation of targeted proteins', Nature Communications, bind 14, 254. https://doi.org/10.1038/s41467-023-35951-0

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abstract = "The current agriculture main challenge is to maintain food production while facing multiple threats such as increasing world population, temperature increase, lack of agrochemicals due to health issues and uprising of weeds resistant to herbicides. Developing novel, alternative, and safe methods is hence of paramount importance. Here, we show that complementary peptides (cPEPs) from any gene can be designed to target specifically plant coding genes. External application of synthetic peptides increases the abundance of the targeted protein, leading to related phenotypes. Moreover, we provide evidence that cPEPs can be powerful tools in agronomy to improve plant traits, such as growth, resistance to pathogen or heat stress, without the needs of genetic approaches. Finally, by combining their activity they can also be used to reduce weed growth.",

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AU - Duboé, Carine

AU - Fabre, Bertrand

AU - Pouzet, Cécile

AU - Impens, Francis

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AU - Carpentier, Marie Christine

AU - Clemente, Hélène San

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AU - Lauressergues, Dominique

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AU - Plaza, Serge

AU - Combier, Jean Philippe

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