Continuous collection of volatiles produced by Streptomyces grown on oatmeal agar by headspace extraction and GC-MS

Raju Podduturi, Nina Clausen, Nanna Bender Petersen, Niels O. G. Jørgensen, Mikael Agerlin Petersen

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Abstract

Volatile organic compounds (VOCs) produced by bacteria are known to play a significant role in interactions among many organisms, but VOCs also impart aroma to many food products. Streptomyces bacteria include a large group of organisms that produce a wide range of secondary volatile metabolites with potential for clinical and industrial applications. VOC profiles of bacteria are typically analyzed in liquid media. Yet, many bacteria also grow well on solid media and may here produce different VOCs than in liquid media. However, it is challenging to capture and measure VOCs from bacteria grown on solid media, and such limitations could bias measured VOCs profiles from bacteria. In this study, a special approach was applied to examine VOC production by Streptomyces when grown on oatmeal agar in a large gas-washing flask with Tenax-TA traps attached 96 hours of incubation. The obtained VOC profiles of two Streptomyces species show presence of geosmin and 2-MIB along with a total of 110 compounds, including 51 terpenes.
OriginalsprogEngelsk
TitelFlavour Science : Proceedings of the XV Weurman Flavour Research Symposium
RedaktørerBarbara Siegmund, Erich Leitner
Antal sider4
ForlagVerlag der Technische Universität Graz
Publikationsdato2018
Sider475-478
ISBN (Trykt)978-3-85125-593-5
DOI
StatusUdgivet - 2018
BegivenhedWeurman Flavour Research Symposium 2017 - Graz University of Technology, Graz, Østrig
Varighed: 18 sep. 201722 sep. 2017
Konferencens nummer: 15
http://www.analytchem.tugraz.at/weurman/index1d98.html?page_id=413

Konference

KonferenceWeurman Flavour Research Symposium 2017
Nummer15
LokationGraz University of Technology
Land/OmrådeØstrig
ByGraz
Periode18/09/201722/09/2017
Internetadresse

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