Abstract
| Originalsprog | Engelsk |
|---|---|
| Tidsskrift | Proceedings of the National Academy of Science of the United States of America |
| Vol/bind | 104 |
| Udgave nummer | 14 |
| Sider (fra-til) | 5830-5 |
| Antal sider | 5 |
| ISSN | 0027-8424 |
| DOI | |
| Status | Udgivet - 2007 |
Bibliografisk note
Keywords: Base Sequence; Biomechanics; Computer Simulation; Escherichia coli; Frameshift Mutation; Frameshifting, Ribosomal; Models, Biological; Molecular Sequence Data; Nucleic Acid Conformation; Optical Tweezers; Plasmids; Protein Biosynthesis; RNA, Messenger; RNA, Viral; Ribosomes; ThermodynamicsAdgang til dokumentet
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I: Proceedings of the National Academy of Science of the United States of America, Bind 104, Nr. 14, 2007, s. 5830-5.
Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › peer review
}
TY - JOUR
T1 - Correlation between mechanical strength of messenger RNA pseudoknots and ribosomal frameshifting.
AU - Hansen, Thomas Møller
AU - Reihani, S Nader S
AU - Oddershede, Lene B
AU - Sørensen, Michael A
N1 - Keywords: Base Sequence; Biomechanics; Computer Simulation; Escherichia coli; Frameshift Mutation; Frameshifting, Ribosomal; Models, Biological; Molecular Sequence Data; Nucleic Acid Conformation; Optical Tweezers; Plasmids; Protein Biosynthesis; RNA, Messenger; RNA, Viral; Ribosomes; Thermodynamics
PY - 2007
Y1 - 2007
N2 - Programmed ribosomal frameshifting is often used by viral pathogens including HIV. Slippery sequences present in some mRNAs cause the ribosome to shift reading frame. The resulting protein is thus encoded by one reading frame upstream from the slippery sequence and by another reading frame downstream from the slippery sequence. Although the mechanism is not well understood, frameshifting is known to be stimulated by an mRNA structure such as a pseudoknot. Here, we show that the efficiency of frameshifting relates to the mechanical strength of the pseudoknot. Two pseudoknots derived from the Infectious Bronchitis Virus were used, differing by one base pair in the first stem. In Escherichia coli, these two pseudoknots caused frameshifting frequencies that differed by a factor of two. We used optical tweezers to unfold the pseudoknots. The pseudoknot giving rise to the highest degree of frameshifting required a nearly 2-fold larger unfolding force than the other. The observed energy difference cannot be accounted for by any existing model. We propose that the degree of ribosomal frameshifting is related to the mechanical strength of RNA pseudoknots. Our observations support the "9 A model" that predicts some physical barrier is needed to force the ribosome into the -1 frame. Also, our findings support the recent observation made by cryoelectron microscopy that mechanical interaction between a ribosome and a pseudoknot causes a deformation of the A-site tRNA. The result has implications for the understanding of genetic regulation, reading frame maintenance, tRNA movement, and unwinding of mRNA secondary structures by ribosomes.
AB - Programmed ribosomal frameshifting is often used by viral pathogens including HIV. Slippery sequences present in some mRNAs cause the ribosome to shift reading frame. The resulting protein is thus encoded by one reading frame upstream from the slippery sequence and by another reading frame downstream from the slippery sequence. Although the mechanism is not well understood, frameshifting is known to be stimulated by an mRNA structure such as a pseudoknot. Here, we show that the efficiency of frameshifting relates to the mechanical strength of the pseudoknot. Two pseudoknots derived from the Infectious Bronchitis Virus were used, differing by one base pair in the first stem. In Escherichia coli, these two pseudoknots caused frameshifting frequencies that differed by a factor of two. We used optical tweezers to unfold the pseudoknots. The pseudoknot giving rise to the highest degree of frameshifting required a nearly 2-fold larger unfolding force than the other. The observed energy difference cannot be accounted for by any existing model. We propose that the degree of ribosomal frameshifting is related to the mechanical strength of RNA pseudoknots. Our observations support the "9 A model" that predicts some physical barrier is needed to force the ribosome into the -1 frame. Also, our findings support the recent observation made by cryoelectron microscopy that mechanical interaction between a ribosome and a pseudoknot causes a deformation of the A-site tRNA. The result has implications for the understanding of genetic regulation, reading frame maintenance, tRNA movement, and unwinding of mRNA secondary structures by ribosomes.
U2 - 10.1073/pnas.0608668104
DO - 10.1073/pnas.0608668104
M3 - Journal article
C2 - 17389398
SN - 0027-8424
VL - 104
SP - 5830
EP - 5835
JO - Proceedings of the National Academy of Science of the United States of America
JF - Proceedings of the National Academy of Science of the United States of America
IS - 14
ER -