Crystallization and preliminary X-ray studies of recombinant amylosucrase from Neisseria polysaccharea

L K Skov; Osman Asghar Mirza; A Henriksen; G Potocki de Montalk; M Remaud-Simeon; P Sarcabal; R M Willemot; P Monsan; M Gajhede

Crystallization and preliminary X-ray studies of recombinant amylosucrase from Neisseria polysaccharea

L K Skov, Osman Asghar Mirza, A Henriksen, G Potocki de Montalk, M Remaud-Simeon, P Sarcabal, R M Willemot, P Monsan, M Gajhede

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › peer review

31 Citationer (Scopus)

Abstract

Recombinant amylosucrase from Neisseria polysaccharea was crystallized by the vapour-diffusion procedure in the presence of polyethylene glycol 6000. The crystals belong to the orthorhombic space group P2(1)2(1)2, with unit-cell parameters a = 95.7, b = 117.2, c = 62.1 A, and diffract to 1.6 A resolution. A p-chloromercuribenzene sulfonate (pcmbs) derivative has been identified and a selenomethionine-substituted protein has been produced and crystallized.

Originalsprog	Engelsk
Tidsskrift	Acta Crystallographica. Section D: Biological Crystallography
Vol/bind	56
Udgave nummer	Pt 2
Sider (fra-til)	203-5
Antal sider	3
ISSN	0907-4449
Status	Udgivet - feb. 2000
Udgivet eksternt	Ja

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title = "Crystallization and preliminary X-ray studies of recombinant amylosucrase from Neisseria polysaccharea",

abstract = "Recombinant amylosucrase from Neisseria polysaccharea was crystallized by the vapour-diffusion procedure in the presence of polyethylene glycol 6000. The crystals belong to the orthorhombic space group P2(1)2(1)2, with unit-cell parameters a = 95.7, b = 117.2, c = 62.1 A, and diffract to 1.6 A resolution. A p-chloromercuribenzene sulfonate (pcmbs) derivative has been identified and a selenomethionine-substituted protein has been produced and crystallized.",

keywords = "Bacterial Proteins, Circular Dichroism, Crystallization, Crystallography, X-Ray, Escherichia coli, Glucosyltransferases, Neisseria, Recombinant Proteins",

author = "Skov, {L K} and Mirza, {Osman Asghar} and A Henriksen and {Potocki de Montalk}, G and M Remaud-Simeon and P Sarcabal and Willemot, {R M} and P Monsan and M Gajhede",

year = "2000",

month = feb,

language = "English",

volume = "56",

pages = "203--5",

journal = "Acta Crystallographica. Section D: Biological Crystallography",

issn = "0907-4449",

publisher = "International Union of Crystallography",

number = "Pt 2",

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TY - JOUR

T1 - Crystallization and preliminary X-ray studies of recombinant amylosucrase from Neisseria polysaccharea

AU - Skov, L K

AU - Mirza, Osman Asghar

AU - Henriksen, A

AU - Potocki de Montalk, G

AU - Remaud-Simeon, M

AU - Sarcabal, P

AU - Willemot, R M

AU - Monsan, P

AU - Gajhede, M

PY - 2000/2

Y1 - 2000/2

N2 - Recombinant amylosucrase from Neisseria polysaccharea was crystallized by the vapour-diffusion procedure in the presence of polyethylene glycol 6000. The crystals belong to the orthorhombic space group P2(1)2(1)2, with unit-cell parameters a = 95.7, b = 117.2, c = 62.1 A, and diffract to 1.6 A resolution. A p-chloromercuribenzene sulfonate (pcmbs) derivative has been identified and a selenomethionine-substituted protein has been produced and crystallized.

AB - Recombinant amylosucrase from Neisseria polysaccharea was crystallized by the vapour-diffusion procedure in the presence of polyethylene glycol 6000. The crystals belong to the orthorhombic space group P2(1)2(1)2, with unit-cell parameters a = 95.7, b = 117.2, c = 62.1 A, and diffract to 1.6 A resolution. A p-chloromercuribenzene sulfonate (pcmbs) derivative has been identified and a selenomethionine-substituted protein has been produced and crystallized.

KW - Bacterial Proteins

KW - Circular Dichroism

KW - Crystallization

KW - Crystallography, X-Ray

KW - Escherichia coli

KW - Glucosyltransferases

KW - Neisseria

KW - Recombinant Proteins

M3 - Journal article

SN - 0907-4449

VL - 56

SP - 203

EP - 205

JO - Acta Crystallographica. Section D: Biological Crystallography

JF - Acta Crystallographica. Section D: Biological Crystallography

IS - Pt 2

ER -