CTL epitopes for influenza A including the H5N1 bird flu; genome-, pathogen-, and HLA-wide screening.

Mingjun Wang; Kasper Lamberth; Mikkel Harndahl; Gustav Røder; Anette Stryhn; Mette V Larsen; Morten Nielsen; Claus Lundegaard; Sheila T Tang; Morten H Dziegiel; Jørgen Rosenkvist; Anders E Pedersen; Søren Buus; Mogens H Claesson; Ole Lund

doi:10.1016/j.vaccine.2006.12.038

CTL epitopes for influenza A including the H5N1 bird flu; genome-, pathogen-, and HLA-wide screening.

Mingjun Wang, Kasper Lamberth, Mikkel Harndahl, Gustav Røder, Anette Stryhn, Mette V Larsen, Morten Nielsen, Claus Lundegaard, Sheila T Tang, Morten H Dziegiel, Jørgen Rosenkvist, Anders E Pedersen, Søren Buus, Mogens H Claesson, Ole Lund

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › peer review

96 Citationer (Scopus)

Abstract

Udgivelsesdato: 2007-Apr-12

Originalsprog	Engelsk
Tidsskrift	Vaccine
Vol/bind	25
Udgave nummer	15
Sider (fra-til)	2823-31
Antal sider	8
ISSN	0264-410X
DOI	https://doi.org/10.1016/j.vaccine.2006.12.038
Status	Udgivet - 2006

Bibliografisk note

Keywords: Adult; Aged; Animals; Birds; Epitopes, T-Lymphocyte; Female; Genetic Screening; Genome, Viral; HLA-A Antigens; HLA-B Antigens; Humans; Influenza A Virus, H5N1 Subtype; Influenza A virus; Influenza in Birds; Influenza, Human; Male; Membrane Transport Proteins; Middle Aged; Proteasome Endopeptidase Complex; T-Lymphocytes, Cytotoxic

Adgang til dokumentet

10.1016/j.vaccine.2006.12.038

Citationsformater

Wang, M., Lamberth, K., Harndahl, M., Røder, G., Stryhn, A., Larsen, M. V., Nielsen, M., Lundegaard, C., Tang, S. T., Dziegiel, M. H., Rosenkvist, J., Pedersen, A. E., Buus, S., Claesson, M. H., & Lund, O. (2006). CTL epitopes for influenza A including the H5N1 bird flu; genome-, pathogen-, and HLA-wide screening. Vaccine, 25(15), 2823-31. https://doi.org/10.1016/j.vaccine.2006.12.038

CTL epitopes for influenza A including the H5N1 bird flu; genome-, pathogen-, and HLA-wide screening. / Wang, Mingjun; Lamberth, Kasper; Harndahl, Mikkel; Røder, Gustav; Stryhn, Anette; Larsen, Mette V; Nielsen, Morten; Lundegaard, Claus; Tang, Sheila T; Dziegiel, Morten H; Rosenkvist, Jørgen; Pedersen, Anders E ; Buus, Søren ; Claesson, Mogens H; Lund, Ole.

I: Vaccine, Bind 25, Nr. 15, 2006, s. 2823-31.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › peer review

Wang, M, Lamberth, K, Harndahl, M, Røder, G, Stryhn, A, Larsen, MV, Nielsen, M, Lundegaard, C, Tang, ST, Dziegiel, MH, Rosenkvist, J, Pedersen, AE , Buus, S , Claesson, MH & Lund, O 2006, 'CTL epitopes for influenza A including the H5N1 bird flu; genome-, pathogen-, and HLA-wide screening.', Vaccine, bind 25, nr. 15, s. 2823-31. https://doi.org/10.1016/j.vaccine.2006.12.038

Wang, Mingjun ; Lamberth, Kasper ; Harndahl, Mikkel ; Røder, Gustav ; Stryhn, Anette ; Larsen, Mette V ; Nielsen, Morten ; Lundegaard, Claus ; Tang, Sheila T ; Dziegiel, Morten H ; Rosenkvist, Jørgen ; Pedersen, Anders E ; Buus, Søren ; Claesson, Mogens H ; Lund, Ole. / CTL epitopes for influenza A including the H5N1 bird flu; genome-, pathogen-, and HLA-wide screening. I: Vaccine. 2006 ; Bind 25, Nr. 15. s. 2823-31.

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title = "CTL epitopes for influenza A including the H5N1 bird flu; genome-, pathogen-, and HLA-wide screening.",

abstract = "The purpose of the present study is to perform a global screening for new immunogenic HLA class I (HLA-I) restricted cytotoxic T cell (CTL) epitopes of potential utility as candidates of influenza A-virus diagnostics and vaccines. We used predictions of antigen processing and presentation, the latter encompassing 12 different HLA class I supertypes with >99% population coverage, and searched for conserved epitopes from available influenza A viral protein sequences. Peptides corresponding to 167 predicted peptide-HLA-I interactions were synthesized, tested for peptide-HLA-I interactions in a biochemical assay and for influenza-specific, HLA-I-restricted CTL responses in an IFN-gamma ELISPOT assay. Eighty-nine peptides could be confirmed as HLA-I binders, and 13 could be confirmed as CTL targets. The 13 epitopes, are highly conserved among human influenza A pathogens, and all of these epitopes are present in the emerging bird flu isolates. Our study demonstrates that present technology enables a fast global screening for T cell immune epitopes of potential diagnostics and vaccine interest. This technology includes immuno-bioinformatics predictors with the capacity to perform fast genome-, pathogen-, and HLA-wide searches for immune targets. To exploit this new potential, a coordinated international effort to analyze the precious source of information represented by rare patients, such as the current victims of bird flu, would be essential.",

author = "Mingjun Wang and Kasper Lamberth and Mikkel Harndahl and Gustav R{\o}der and Anette Stryhn and Larsen, {Mette V} and Morten Nielsen and Claus Lundegaard and Tang, {Sheila T} and Dziegiel, {Morten H} and J{\o}rgen Rosenkvist and Pedersen, {Anders E} and S{\o}ren Buus and Claesson, {Mogens H} and Ole Lund",

note = "Keywords: Adult; Aged; Animals; Birds; Epitopes, T-Lymphocyte; Female; Genetic Screening; Genome, Viral; HLA-A Antigens; HLA-B Antigens; Humans; Influenza A Virus, H5N1 Subtype; Influenza A virus; Influenza in Birds; Influenza, Human; Male; Membrane Transport Proteins; Middle Aged; Proteasome Endopeptidase Complex; T-Lymphocytes, Cytotoxic",

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T1 - CTL epitopes for influenza A including the H5N1 bird flu; genome-, pathogen-, and HLA-wide screening.

AU - Wang, Mingjun

AU - Lamberth, Kasper

AU - Harndahl, Mikkel

AU - Røder, Gustav

AU - Stryhn, Anette

AU - Larsen, Mette V

AU - Nielsen, Morten

AU - Lundegaard, Claus

AU - Tang, Sheila T

AU - Dziegiel, Morten H

AU - Rosenkvist, Jørgen

AU - Pedersen, Anders E

AU - Buus, Søren

AU - Claesson, Mogens H

AU - Lund, Ole

N1 - Keywords: Adult; Aged; Animals; Birds; Epitopes, T-Lymphocyte; Female; Genetic Screening; Genome, Viral; HLA-A Antigens; HLA-B Antigens; Humans; Influenza A Virus, H5N1 Subtype; Influenza A virus; Influenza in Birds; Influenza, Human; Male; Membrane Transport Proteins; Middle Aged; Proteasome Endopeptidase Complex; T-Lymphocytes, Cytotoxic

PY - 2006

Y1 - 2006

N2 - The purpose of the present study is to perform a global screening for new immunogenic HLA class I (HLA-I) restricted cytotoxic T cell (CTL) epitopes of potential utility as candidates of influenza A-virus diagnostics and vaccines. We used predictions of antigen processing and presentation, the latter encompassing 12 different HLA class I supertypes with >99% population coverage, and searched for conserved epitopes from available influenza A viral protein sequences. Peptides corresponding to 167 predicted peptide-HLA-I interactions were synthesized, tested for peptide-HLA-I interactions in a biochemical assay and for influenza-specific, HLA-I-restricted CTL responses in an IFN-gamma ELISPOT assay. Eighty-nine peptides could be confirmed as HLA-I binders, and 13 could be confirmed as CTL targets. The 13 epitopes, are highly conserved among human influenza A pathogens, and all of these epitopes are present in the emerging bird flu isolates. Our study demonstrates that present technology enables a fast global screening for T cell immune epitopes of potential diagnostics and vaccine interest. This technology includes immuno-bioinformatics predictors with the capacity to perform fast genome-, pathogen-, and HLA-wide searches for immune targets. To exploit this new potential, a coordinated international effort to analyze the precious source of information represented by rare patients, such as the current victims of bird flu, would be essential.

AB - The purpose of the present study is to perform a global screening for new immunogenic HLA class I (HLA-I) restricted cytotoxic T cell (CTL) epitopes of potential utility as candidates of influenza A-virus diagnostics and vaccines. We used predictions of antigen processing and presentation, the latter encompassing 12 different HLA class I supertypes with >99% population coverage, and searched for conserved epitopes from available influenza A viral protein sequences. Peptides corresponding to 167 predicted peptide-HLA-I interactions were synthesized, tested for peptide-HLA-I interactions in a biochemical assay and for influenza-specific, HLA-I-restricted CTL responses in an IFN-gamma ELISPOT assay. Eighty-nine peptides could be confirmed as HLA-I binders, and 13 could be confirmed as CTL targets. The 13 epitopes, are highly conserved among human influenza A pathogens, and all of these epitopes are present in the emerging bird flu isolates. Our study demonstrates that present technology enables a fast global screening for T cell immune epitopes of potential diagnostics and vaccine interest. This technology includes immuno-bioinformatics predictors with the capacity to perform fast genome-, pathogen-, and HLA-wide searches for immune targets. To exploit this new potential, a coordinated international effort to analyze the precious source of information represented by rare patients, such as the current victims of bird flu, would be essential.

U2 - 10.1016/j.vaccine.2006.12.038

DO - 10.1016/j.vaccine.2006.12.038

M3 - Journal article

C2 - 17254671

VL - 25

SP - 2823

EP - 2831

JO - Vaccine

JF - Vaccine

SN - 0264-410X

IS - 15

ER -