@article{2a0bf900acc711ddb538000ea68e967b,
title = "Culture independent PCR: an alternative enzyme discovery strategy.",
abstract = "Degenerate primers were designed for use in a culture-independent PCR screening of DNA from composite fungal communities, inhabiting residues of corn stovers and leaves. According to similarity searches and alignments amplified clone sequences affiliated with glycosyl hydrolase family 7 and glycosyl hydrolase family 45 though significant sequence divergence was observed. Glycosyl hydrolases from families 7 and 45 play a crucial role in biomass conversion to fuel ethanol. Research in this renewable energy source has two objectives: (i) To contribute to development of a renewable alternative to world's limited crude fossil oil reserves and (ii) to reduce air pollution. Amplification with 18S rDNA-specific primers revealed species within the ascomycetous orders Sordariales and Hypocreales as well as basidiomycetous order Agaricales to be present in these communities. Our study documents the value of culture-independent PCR in microbial diversity studies and could add to development of a new enzyme screening technology.",
author = "Jonas Jacobsen and Magnus Lydolph and Lene Lange",
note = "Keywords: Amino Acid Sequence; Ascomycota; Base Sequence; Basidiomycota; Cellulase; Cellulose 1,4-beta-Cellobiosidase; Cloning, Molecular; DNA, Fungal; Molecular Sequence Data; Phylogeny; Plant Leaves; Polymerase Chain Reaction; Sequence Alignment; Zea mays",
year = "2005",
doi = "10.1016/j.mimet.2004.08.013",
language = "English",
volume = "60",
pages = "63--71",
journal = "Journal of Microbiological Methods",
issn = "0167-7012",
publisher = "Elsevier",
number = "1",
}