Cyclooxygenase-2 immunoreactivity in collagenous colitis

Signe Wildt; Jüri J Rumessen; Claudio Csillag; Michel Normark; Kristian Arild Poulsen; Miriam Kolko; Signe Wildt; Jüri J Rumessen; Claudio Csillag; Michel Normark; Kristian Arild Poulsen; Miriam Kolko

doi:10.1111/j.1600-0463.2009.02447.x

Cyclooxygenase-2 immunoreactivity in collagenous colitis

Signe Wildt, Jüri J Rumessen, Claudio Csillag, Michel Normark, Kristian Arild Poulsen, Miriam Kolko, Signe Wildt, Jüri J Rumessen, Claudio Csillag, Michel Normark, Kristian Arild Poulsen, Miriam Kolko

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › peer review

8 Citationer (Scopus)

Abstract

Udgivelsesdato: 2009-Jul

Originalsprog	Engelsk
Tidsskrift	Acta Pathologica Microbiologica et Immunologica Scandinavica
Vol/bind	117
Udgave nummer	7
Sider (fra-til)	500-6
Antal sider	7
ISSN	0903-4641
DOI	https://doi.org/10.1111/j.1600-0463.2009.02447.x https://doi.org/10.1111/j.1600-0463.2009.02447.x
Status	Udgivet - 1 jul. 2009

Bibliografisk note

Keywords: Adult; Aged; Biopsy; Blotting, Western; Cell Count; Colitis, Collagenous; Cyclooxygenase 2; Female; Humans; Immunohistochemistry; Intestinal Mucosa; Microscopy, Fluorescence; Middle Aged

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Citationsformater

Wildt, S., Rumessen, J. J., Csillag, C., Normark, M., Poulsen, K. A., Kolko, M., Wildt, S., Rumessen, J. J., Csillag, C., Normark, M., Poulsen, K. A., & Kolko, M. (2009). Cyclooxygenase-2 immunoreactivity in collagenous colitis. Acta Pathologica Microbiologica et Immunologica Scandinavica, 117(7), 500-6. https://doi.org/10.1111/j.1600-0463.2009.02447.x, https://doi.org/10.1111/j.1600-0463.2009.02447.x

Wildt, S, Rumessen, JJ, Csillag, C, Normark, M, Poulsen, KA, Kolko, M, Wildt, S, Rumessen, JJ, Csillag, C, Normark, M, Poulsen, KA & Kolko, M 2009, 'Cyclooxygenase-2 immunoreactivity in collagenous colitis', Acta Pathologica Microbiologica et Immunologica Scandinavica, bind 117, nr. 7, s. 500-6. https://doi.org/10.1111/j.1600-0463.2009.02447.x, https://doi.org/10.1111/j.1600-0463.2009.02447.x

@article{c13a7020791911df928f000ea68e967b,

title = "Cyclooxygenase-2 immunoreactivity in collagenous colitis",

abstract = "Collagenous colitis (CC) is an inflammatory bowel disease of unknown aetiology and pathogenesis. In ulcerative colitis and Crohn's disease, prostaglandins may be involved in the pathogenesis of inflammation, and increased expression of cyclo-oxygenase-2 (COX-2) has been detected. The purpose of this study was to examine the presence and cellular localization of COX-2 in colonic mucosa of patients with CC. Using immunohistochemistry, immunoflouresence and Western blot analysis, COX-2 expression was evaluated in colonic mucosal biopsies from 10 patients with active untreated CC, and compared with samples from eight normal controls, and samples from eight patients with ulcerative colitis or Crohn's disease. Specimens from patients with CC expressed COX-2 protein in increased amounts compared with controls, but similar to patients with ulcerative colitis and Crohn's disease. COX-2 expression was localized to the mononuclear cells of the lamina propria. COX-2 expression was most evident in macrophages. Co-localization of COX-2 and macrophages was increased in number in comparison with controls. In conclusion COX-2 is expressed in increased amounts primarily in the macrophage subpopulation of the inflammatory infiltrate of lamina propria in CC. Increased recruitment of macrophages, increased expression of COX-2 and increased prostaglandin synthesis may be involved in the pathogenesis of CC.",

author = "Signe Wildt and Rumessen, {J{\"u}ri J} and Claudio Csillag and Michel Normark and Poulsen, {Kristian Arild} and Miriam Kolko and Signe Wildt and Rumessen, {J{\"u}ri J} and Claudio Csillag and Michel Normark and Poulsen, {Kristian Arild} and Miriam Kolko",

note = "Keywords: Adult; Aged; Biopsy; Blotting, Western; Cell Count; Colitis, Collagenous; Cyclooxygenase 2; Female; Humans; Immunohistochemistry; Intestinal Mucosa; Microscopy, Fluorescence; Middle Aged",

year = "2009",

month = jul,

day = "1",

doi = "10.1111/j.1600-0463.2009.02447.x",

language = "English",

volume = "117",

pages = "500--6",

journal = "Acta Pathologica Microbiologica et Immunologica Scandinavica",

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TY - JOUR

T1 - Cyclooxygenase-2 immunoreactivity in collagenous colitis

AU - Wildt, Signe

AU - Rumessen, Jüri J

AU - Csillag, Claudio

AU - Normark, Michel

AU - Poulsen, Kristian Arild

AU - Kolko, Miriam

AU - Wildt, Signe

AU - Rumessen, Jüri J

AU - Csillag, Claudio

AU - Normark, Michel

AU - Poulsen, Kristian Arild

AU - Kolko, Miriam

N1 - Keywords: Adult; Aged; Biopsy; Blotting, Western; Cell Count; Colitis, Collagenous; Cyclooxygenase 2; Female; Humans; Immunohistochemistry; Intestinal Mucosa; Microscopy, Fluorescence; Middle Aged

PY - 2009/7/1

Y1 - 2009/7/1

N2 - Collagenous colitis (CC) is an inflammatory bowel disease of unknown aetiology and pathogenesis. In ulcerative colitis and Crohn's disease, prostaglandins may be involved in the pathogenesis of inflammation, and increased expression of cyclo-oxygenase-2 (COX-2) has been detected. The purpose of this study was to examine the presence and cellular localization of COX-2 in colonic mucosa of patients with CC. Using immunohistochemistry, immunoflouresence and Western blot analysis, COX-2 expression was evaluated in colonic mucosal biopsies from 10 patients with active untreated CC, and compared with samples from eight normal controls, and samples from eight patients with ulcerative colitis or Crohn's disease. Specimens from patients with CC expressed COX-2 protein in increased amounts compared with controls, but similar to patients with ulcerative colitis and Crohn's disease. COX-2 expression was localized to the mononuclear cells of the lamina propria. COX-2 expression was most evident in macrophages. Co-localization of COX-2 and macrophages was increased in number in comparison with controls. In conclusion COX-2 is expressed in increased amounts primarily in the macrophage subpopulation of the inflammatory infiltrate of lamina propria in CC. Increased recruitment of macrophages, increased expression of COX-2 and increased prostaglandin synthesis may be involved in the pathogenesis of CC.

AB - Collagenous colitis (CC) is an inflammatory bowel disease of unknown aetiology and pathogenesis. In ulcerative colitis and Crohn's disease, prostaglandins may be involved in the pathogenesis of inflammation, and increased expression of cyclo-oxygenase-2 (COX-2) has been detected. The purpose of this study was to examine the presence and cellular localization of COX-2 in colonic mucosa of patients with CC. Using immunohistochemistry, immunoflouresence and Western blot analysis, COX-2 expression was evaluated in colonic mucosal biopsies from 10 patients with active untreated CC, and compared with samples from eight normal controls, and samples from eight patients with ulcerative colitis or Crohn's disease. Specimens from patients with CC expressed COX-2 protein in increased amounts compared with controls, but similar to patients with ulcerative colitis and Crohn's disease. COX-2 expression was localized to the mononuclear cells of the lamina propria. COX-2 expression was most evident in macrophages. Co-localization of COX-2 and macrophages was increased in number in comparison with controls. In conclusion COX-2 is expressed in increased amounts primarily in the macrophage subpopulation of the inflammatory infiltrate of lamina propria in CC. Increased recruitment of macrophages, increased expression of COX-2 and increased prostaglandin synthesis may be involved in the pathogenesis of CC.

U2 - 10.1111/j.1600-0463.2009.02447.x

DO - 10.1111/j.1600-0463.2009.02447.x

M3 - Journal article

C2 - 19594490

SN - 0903-4641

VL - 117

SP - 500

EP - 506

JO - Acta Pathologica Microbiologica et Immunologica Scandinavica

JF - Acta Pathologica Microbiologica et Immunologica Scandinavica

IS - 7

ER -