Cytokine signalling in embryonic stem cells.

David Møbjerg Kristensen; Mark Kalisz; Jens Høiriis Nielsen

doi:10.1111/j.1600-0463.2005.apm_391.x

Cytokine signalling in embryonic stem cells.

David Møbjerg Kristensen, Mark Kalisz, Jens Høiriis Nielsen

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › peer review

69 Citationer (Scopus)

Abstract

Udgivelsesdato: null-null

Originalsprog	Engelsk
Tidsskrift	Acta Pathologica Microbiologica et Immunologica Scandinavica
Vol/bind	113
Udgave nummer	11-12
Sider (fra-til)	756-72
Antal sider	16
ISSN	0903-4641
DOI	https://doi.org/10.1111/j.1600-0463.2005.apm_391.x
Status	Udgivet - 2006

Bibliografisk note

Keywords: Animals; Cell Differentiation; Cytokines; Embryo, Mammalian; Humans; Interleukin-6; Leukemia Inhibitory Factor; Mice; Pluripotent Stem Cells; Protein-Tyrosine Kinases; STAT3 Transcription Factor; Signal Transduction

Adgang til dokumentet

10.1111/j.1600-0463.2005.apm_391.x

Citationsformater

@article{a9c38430acd411ddb538000ea68e967b,

title = "Cytokine signalling in embryonic stem cells.",

abstract = "Cytokines play a central role in maintaining self-renewal in mouse embryonic stem (ES) cells through a member of the interleukin-6 type cytokine family termed leukemia inhibitory factor (LIF). LIF activates the JAK-STAT3 pathway through the class I cytokine receptor gp130, which forms a trimeric complex with LIF and the class I cytokine receptor LIF receptor beta. STAT3 has been shown to play a crucial role in self-renewal in mouse ES cells probably by induction of c-myc expression. Thus, ablation of STAT3 activation leads to differentiation. However, important connections between STAT3 and other signalling pathways have been documented. In addition, gp130 activation leads to both PI3K and Src activation. The canonical Wnt pathway is sufficient to maintain self-renewal of both human ES cells and mouse ES cells. It seems quite possible that the main pathway maintaining self-renewal in ES cells is the Wnt pathway, while the LIF-JAK-STAT3 pathway is present in mouse cells as an adaptation for sustaining self-renewal during embryonic diapause, a condition of delayed implantation in mammals. In keeping with this scenario, the Wnt pathway has been shown to elevate the level of c-myc. Thus, the two pathways seem to converge on c-myc as a common target to promote self-renewal. Whereas LIF does not seem to stimulate self-renewal in human embryonic stem cells it cannot be excluded that other cytokines are involved. The pleiotropic actions of the increasing number of cytokines and receptors signalling via JAKs, STATs and SOCS exhibit considerable redundancy, compensation and plasticity in stem cells in accordance with the view that stem cells are governed by quantitative variations in strength and duration of signalling events known from other cell types rather than qualitatively different stem cell-specific factors.",

author = "Kristensen, {David M{\o}bjerg} and Mark Kalisz and Nielsen, {Jens H{\o}iriis}",

note = "Keywords: Animals; Cell Differentiation; Cytokines; Embryo, Mammalian; Humans; Interleukin-6; Leukemia Inhibitory Factor; Mice; Pluripotent Stem Cells; Protein-Tyrosine Kinases; STAT3 Transcription Factor; Signal Transduction",

year = "2006",

doi = "10.1111/j.1600-0463.2005.apm_391.x",

language = "English",

volume = "113",

pages = "756--72",

journal = "A P M I S. Acta Pathologica, Microbiologica et Immunologica Scandinavica",

issn = "0903-4641",

publisher = "Wiley Online",

number = "11-12",

}

TY - JOUR

T1 - Cytokine signalling in embryonic stem cells.

AU - Kristensen, David Møbjerg

AU - Kalisz, Mark

AU - Nielsen, Jens Høiriis

N1 - Keywords: Animals; Cell Differentiation; Cytokines; Embryo, Mammalian; Humans; Interleukin-6; Leukemia Inhibitory Factor; Mice; Pluripotent Stem Cells; Protein-Tyrosine Kinases; STAT3 Transcription Factor; Signal Transduction

PY - 2006

Y1 - 2006

N2 - Cytokines play a central role in maintaining self-renewal in mouse embryonic stem (ES) cells through a member of the interleukin-6 type cytokine family termed leukemia inhibitory factor (LIF). LIF activates the JAK-STAT3 pathway through the class I cytokine receptor gp130, which forms a trimeric complex with LIF and the class I cytokine receptor LIF receptor beta. STAT3 has been shown to play a crucial role in self-renewal in mouse ES cells probably by induction of c-myc expression. Thus, ablation of STAT3 activation leads to differentiation. However, important connections between STAT3 and other signalling pathways have been documented. In addition, gp130 activation leads to both PI3K and Src activation. The canonical Wnt pathway is sufficient to maintain self-renewal of both human ES cells and mouse ES cells. It seems quite possible that the main pathway maintaining self-renewal in ES cells is the Wnt pathway, while the LIF-JAK-STAT3 pathway is present in mouse cells as an adaptation for sustaining self-renewal during embryonic diapause, a condition of delayed implantation in mammals. In keeping with this scenario, the Wnt pathway has been shown to elevate the level of c-myc. Thus, the two pathways seem to converge on c-myc as a common target to promote self-renewal. Whereas LIF does not seem to stimulate self-renewal in human embryonic stem cells it cannot be excluded that other cytokines are involved. The pleiotropic actions of the increasing number of cytokines and receptors signalling via JAKs, STATs and SOCS exhibit considerable redundancy, compensation and plasticity in stem cells in accordance with the view that stem cells are governed by quantitative variations in strength and duration of signalling events known from other cell types rather than qualitatively different stem cell-specific factors.

AB - Cytokines play a central role in maintaining self-renewal in mouse embryonic stem (ES) cells through a member of the interleukin-6 type cytokine family termed leukemia inhibitory factor (LIF). LIF activates the JAK-STAT3 pathway through the class I cytokine receptor gp130, which forms a trimeric complex with LIF and the class I cytokine receptor LIF receptor beta. STAT3 has been shown to play a crucial role in self-renewal in mouse ES cells probably by induction of c-myc expression. Thus, ablation of STAT3 activation leads to differentiation. However, important connections between STAT3 and other signalling pathways have been documented. In addition, gp130 activation leads to both PI3K and Src activation. The canonical Wnt pathway is sufficient to maintain self-renewal of both human ES cells and mouse ES cells. It seems quite possible that the main pathway maintaining self-renewal in ES cells is the Wnt pathway, while the LIF-JAK-STAT3 pathway is present in mouse cells as an adaptation for sustaining self-renewal during embryonic diapause, a condition of delayed implantation in mammals. In keeping with this scenario, the Wnt pathway has been shown to elevate the level of c-myc. Thus, the two pathways seem to converge on c-myc as a common target to promote self-renewal. Whereas LIF does not seem to stimulate self-renewal in human embryonic stem cells it cannot be excluded that other cytokines are involved. The pleiotropic actions of the increasing number of cytokines and receptors signalling via JAKs, STATs and SOCS exhibit considerable redundancy, compensation and plasticity in stem cells in accordance with the view that stem cells are governed by quantitative variations in strength and duration of signalling events known from other cell types rather than qualitatively different stem cell-specific factors.

U2 - 10.1111/j.1600-0463.2005.apm_391.x

DO - 10.1111/j.1600-0463.2005.apm_391.x

M3 - Journal article

C2 - 16480448

VL - 113

SP - 756

EP - 772

JO - A P M I S. Acta Pathologica, Microbiologica et Immunologica Scandinavica

JF - A P M I S. Acta Pathologica, Microbiologica et Immunologica Scandinavica

SN - 0903-4641

IS - 11-12

ER -