DEAD box protein DDX1 regulates cytoplasmic localization of KSRP

Chu-Fang Chou, Wei-Jye Lin, Chen-Chung Lin, Christian A Luber, Roseline Godbout, Matthias Mann, Ching-Yi Chen

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13 Citationer (Scopus)

Abstract

mRNA decay mediated by the AU-rich elements (AREs) is one of the most studied post-transcriptional mechanisms and is modulated by ARE-binding proteins (ARE-BPs). To understand the regulation of K homology splicing regulatory protein (KSRP), a decay-promoting ARE-BP, we purified KSRP protein complexes and identified an RNA helicase, DDX1. We showed that down-regulation of DDX1 expression elevated cytoplasmic levels of KSRP and facilitated ARE-mediated mRNA decay. Association of KSRP with 14-3-3 proteins, that are predominately located in the cytoplasm, increased upon reduction of DDX1. We also demonstrated that KSRP associated with DDX1 or 14-3-3, but not both. These observations indicate that subcellular localization of KSRP is regulated by competing interactions with DDX1 or 14-3-3.
OriginalsprogEngelsk
Artikelnummere73752
TidsskriftP L o S One
Vol/bind8
Udgave nummer9
ISSN1932-6203
DOI
StatusUdgivet - 4 sep. 2013
Udgivet eksterntJa

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