Deep immune cell phenotyping and induced immune cell responses at admission stratified by BMI in patients hospitalized with COVID-19: An observational multicenter cohort pilot study

Adin Sejdic; Hans Jakob Hartling; Jon Gitz Holler; Lars Klingen Gjærde; Arnold Matovu Dungu; Maria Elizabeth Engel Møller; Rebecca Svanberg Teglgaard; Carsten Utoft Utoft Niemann; Patrick Terrence Brooks; Trine H. Mogensen; Nina Weis; Daria Podlekareva; Marie Louise Baum Jørgensen; Anne Ortved Gang; Ditte Stampe Hersby; Annemette Hald; Susanne Dam Nielsen; Anne Mette Lebech; Marie Helleberg; Jens Lundgren; Kristina Træholt Franck; Thea K. Fischer; Zitta Barrella Harboe; Hanne Vibeke Marquart; Sisse Rye Ostrowski; Birgitte Lindegaard

doi:10.1016/j.clim.2024.110336

Deep immune cell phenotyping and induced immune cell responses at admission stratified by BMI in patients hospitalized with COVID-19: An observational multicenter cohort pilot study

Adin Sejdic^*, Hans Jakob Hartling, Jon Gitz Holler, Lars Klingen Gjærde, Arnold Matovu Dungu, Maria Elizabeth Engel Møller, Rebecca Svanberg Teglgaard, Carsten Utoft Utoft Niemann, Patrick Terrence Brooks, Trine H. Mogensen, Nina Weis, Daria Podlekareva, Marie Louise Baum Jørgensen, Anne Ortved Gang, Ditte Stampe Hersby, Annemette Hald, Susanne Dam Nielsen, Anne Mette Lebech, Marie Helleberg, Jens LundgrenKristina Træholt Franck, Thea K. Fischer, Zitta Barrella Harboe, Hanne Vibeke Marquart, Sisse Rye Ostrowski, Birgitte Lindegaard

^*Corresponding author af dette arbejde

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › peer review

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Abstract

Introduction
Overweight and obesity are linked to increased hospitalization and mortality in COVID-19 patients. This study aimed to characterize induced immune responses and deep immune cell profiles stratified by BMI in hospitalized COVID-19 patients.

Methods and results
This observational multicenter cohort pilot study included 122 adult patients with PCR-confirmed COVID-19 in Denmark, stratified by BMI (normal weight, overweight, obese). Inflammation was assessed using TruCulture® and immune cell profiles by flow cytometry with a customized antibody panel (DuraClone®). Patients with obesity had a more pro-inflammatory phenotype with increased TNF-α, IL-8, IL-17, and IL-10 levels post-T cell stimulation, and altered B cell profiles. Patients with obesity showed higher concentrations of naïve, transitional, and non-isotype switched memory B cells, and plasmablasts compared to normal weight patients and healthy controls.

Conclusions
Obesity in hospitalized COVID-19 patients may correlate with elevated pro-inflammatory cytokines, anti-inflammatory IL-10, and increased B cell subset activation, highlighting the need for further studies.

Originalsprog	Engelsk
Artikelnummer	110336
Tidsskrift	Clinical Immunology
Vol/bind	267
Antal sider	15
ISSN	1521-6616
DOI	https://doi.org/10.1016/j.clim.2024.110336
Status	Udgivet - 2024

Bibliografisk note

Funding Information:
The study was supported by a grant from the Ministry of Higher Education and Science ( 0238-00006B ), Lundbeck Foundation ( R349-2020-835 ) and the Danish National Research Foundation (PERSIMUNE study grant).

Publisher Copyright:
© 2024

Adgang til dokumentet

10.1016/j.clim.2024.110336Licens: CC BY

FulltextForlagets udgivne version, 8,85 MBLicens: CC BY

Citationsformater

Sejdic, A., Hartling, H. J., Gitz Holler, J., Klingen Gjærde, L., Matovu Dungu, A., Engel Møller, M. E., Svanberg Teglgaard, R., Utoft Niemann, C. U., Brooks, P. T., Mogensen, T. H., Weis, N., Podlekareva, D., Baum Jørgensen, M. L., Ortved Gang, A., Stampe Hersby, D., Hald, A., Dam Nielsen, S., Lebech, A. M., Helleberg, M., ... Lindegaard, B. (2024). Deep immune cell phenotyping and induced immune cell responses at admission stratified by BMI in patients hospitalized with COVID-19: An observational multicenter cohort pilot study. Clinical Immunology, 267, Artikel 110336. https://doi.org/10.1016/j.clim.2024.110336

Deep immune cell phenotyping and induced immune cell responses at admission stratified by BMI in patients hospitalized with COVID-19: An observational multicenter cohort pilot study. / Sejdic, Adin; Hartling, Hans Jakob; Gitz Holler, Jon et al.
I: Clinical Immunology, Bind 267, 110336, 2024.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › peer review

Sejdic, A, Hartling, HJ, Gitz Holler, J, Klingen Gjærde, L, Matovu Dungu, A, Engel Møller, ME, Svanberg Teglgaard, R, Utoft Niemann, CU, Brooks, PT, Mogensen, TH, Weis, N , Podlekareva, D, Baum Jørgensen, ML, Ortved Gang, A, Stampe Hersby, D, Hald, A, Dam Nielsen, S , Lebech, AM , Helleberg, M , Lundgren, J, Træholt Franck, K, Fischer, TK , Barrella Harboe, Z , Marquart, HV , Rye Ostrowski, S & Lindegaard, B 2024, 'Deep immune cell phenotyping and induced immune cell responses at admission stratified by BMI in patients hospitalized with COVID-19: An observational multicenter cohort pilot study', Clinical Immunology, bind 267, 110336. https://doi.org/10.1016/j.clim.2024.110336

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title = "Deep immune cell phenotyping and induced immune cell responses at admission stratified by BMI in patients hospitalized with COVID-19: An observational multicenter cohort pilot study",

abstract = "Introduction: Overweight and obesity are linked to increased hospitalization and mortality in COVID-19 patients. This study aimed to characterize induced immune responses and deep immune cell profiles stratified by BMI in hospitalized COVID-19 patients. Methods and results: This observational multicenter cohort pilot study included 122 adult patients with PCR-confirmed COVID-19 in Denmark, stratified by BMI (normal weight, overweight, obese). Inflammation was assessed using TruCulture{\textregistered} and immune cell profiles by flow cytometry with a customized antibody panel (DuraClone{\textregistered}). Patients with obesity had a more pro-inflammatory phenotype with increased TNF-α, IL-8, IL-17, and IL-10 levels post-T cell stimulation, and altered B cell profiles. Patients with obesity showed higher concentrations of na{\"i}ve, transitional, and non-isotype switched memory B cells, and plasmablasts compared to normal weight patients and healthy controls. Conclusions: Obesity in hospitalized COVID-19 patients may correlate with elevated pro-inflammatory cytokines, anti-inflammatory IL-10, and increased B cell subset activation, highlighting the need for further studies.",

keywords = "Adaptive immunity, B cells, BMI, COVID-19, Cytokines, Inflammation, Innate immunity, Obesity, SARS-CoV-2, T cells",

author = "Adin Sejdic and Hartling, {Hans Jakob} and {Gitz Holler}, Jon and {Klingen Gj{\ae}rde}, Lars and {Matovu Dungu}, Arnold and {Engel M{\o}ller}, {Maria Elizabeth} and {Svanberg Teglgaard}, Rebecca and {Utoft Niemann}, {Carsten Utoft} and Brooks, {Patrick Terrence} and Mogensen, {Trine H.} and Nina Weis and Daria Podlekareva and {Baum J{\o}rgensen}, {Marie Louise} and {Ortved Gang}, Anne and {Stampe Hersby}, Ditte and Annemette Hald and {Dam Nielsen}, Susanne and Lebech, {Anne Mette} and Marie Helleberg and Jens Lundgren and {Tr{\ae}holt Franck}, Kristina and Fischer, {Thea K.} and {Barrella Harboe}, Zitta and Marquart, {Hanne Vibeke} and {Rye Ostrowski}, Sisse and Birgitte Lindegaard",

note = "Publisher Copyright: {\textcopyright} 2024",

year = "2024",

doi = "10.1016/j.clim.2024.110336",

language = "English",

volume = "267",

journal = "Clinical Immunology",

issn = "1521-6616",

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TY - JOUR

T1 - Deep immune cell phenotyping and induced immune cell responses at admission stratified by BMI in patients hospitalized with COVID-19

T2 - An observational multicenter cohort pilot study

AU - Sejdic, Adin

AU - Hartling, Hans Jakob

AU - Gitz Holler, Jon

AU - Klingen Gjærde, Lars

AU - Matovu Dungu, Arnold

AU - Engel Møller, Maria Elizabeth

AU - Svanberg Teglgaard, Rebecca

AU - Utoft Niemann, Carsten Utoft

AU - Brooks, Patrick Terrence

AU - Mogensen, Trine H.

AU - Weis, Nina

AU - Podlekareva, Daria

AU - Baum Jørgensen, Marie Louise

AU - Ortved Gang, Anne

AU - Stampe Hersby, Ditte

AU - Hald, Annemette

AU - Dam Nielsen, Susanne

AU - Lebech, Anne Mette

AU - Helleberg, Marie

AU - Lundgren, Jens

AU - Træholt Franck, Kristina

AU - Fischer, Thea K.

AU - Barrella Harboe, Zitta

AU - Marquart, Hanne Vibeke

AU - Rye Ostrowski, Sisse

AU - Lindegaard, Birgitte

PY - 2024

Y1 - 2024

N2 - Introduction: Overweight and obesity are linked to increased hospitalization and mortality in COVID-19 patients. This study aimed to characterize induced immune responses and deep immune cell profiles stratified by BMI in hospitalized COVID-19 patients. Methods and results: This observational multicenter cohort pilot study included 122 adult patients with PCR-confirmed COVID-19 in Denmark, stratified by BMI (normal weight, overweight, obese). Inflammation was assessed using TruCulture® and immune cell profiles by flow cytometry with a customized antibody panel (DuraClone®). Patients with obesity had a more pro-inflammatory phenotype with increased TNF-α, IL-8, IL-17, and IL-10 levels post-T cell stimulation, and altered B cell profiles. Patients with obesity showed higher concentrations of naïve, transitional, and non-isotype switched memory B cells, and plasmablasts compared to normal weight patients and healthy controls. Conclusions: Obesity in hospitalized COVID-19 patients may correlate with elevated pro-inflammatory cytokines, anti-inflammatory IL-10, and increased B cell subset activation, highlighting the need for further studies.

AB - Introduction: Overweight and obesity are linked to increased hospitalization and mortality in COVID-19 patients. This study aimed to characterize induced immune responses and deep immune cell profiles stratified by BMI in hospitalized COVID-19 patients. Methods and results: This observational multicenter cohort pilot study included 122 adult patients with PCR-confirmed COVID-19 in Denmark, stratified by BMI (normal weight, overweight, obese). Inflammation was assessed using TruCulture® and immune cell profiles by flow cytometry with a customized antibody panel (DuraClone®). Patients with obesity had a more pro-inflammatory phenotype with increased TNF-α, IL-8, IL-17, and IL-10 levels post-T cell stimulation, and altered B cell profiles. Patients with obesity showed higher concentrations of naïve, transitional, and non-isotype switched memory B cells, and plasmablasts compared to normal weight patients and healthy controls. Conclusions: Obesity in hospitalized COVID-19 patients may correlate with elevated pro-inflammatory cytokines, anti-inflammatory IL-10, and increased B cell subset activation, highlighting the need for further studies.

KW - Adaptive immunity

KW - B cells

KW - BMI

KW - COVID-19

KW - Cytokines

KW - Inflammation

KW - Innate immunity

KW - Obesity

KW - SARS-CoV-2

KW - T cells

U2 - 10.1016/j.clim.2024.110336

DO - 10.1016/j.clim.2024.110336

M3 - Journal article

C2 - 39117044

AN - SCOPUS:85201002798

SN - 1521-6616

VL - 267

JO - Clinical Immunology

JF - Clinical Immunology

M1 - 110336

ER -