Detection and Characterization of Integrons, Transposons, Plasmids and Genomic types in Multidrug Resistant Clinical Isolates of Salmonella enterica Serovar Virchow

I. Rodríguez; A. Herrero; N. Martí; M. C. Martín; M. R. Rodicio; M. C. Mendoza

doi:10.1002/9783527611904.ch123

Detection and Characterization of Integrons, Transposons, Plasmids and Genomic types in Multidrug Resistant Clinical Isolates of Salmonella enterica Serovar Virchow

I. Rodríguez, A. Herrero, N. Martí, M. C. Martín, M. R. Rodicio, M. C. Mendoza^*

^*Corresponding author af dette arbejde

Publikation: Bidrag til bog/antologi/rapport › Bidrag til bog/antologi › Forskning › peer review

Abstract

Thirteen multidrug resistant (to 7 or more antimicrobial drugs) isolates of Salmonella enterica sv Virchow were analyzed by different molecular approaches. PCR amplification allowed the detection of several resistance (R) determinants and of integron and transposon sequences. The isolates were grouped into 11 resistance genotypes and 4 integron profiles. All of them were positive for class 1 integrons (with 3 different arrays of gene cassettes in the variable regions), and three isolates also contained the same class 2 integron. Specific sequences of transposons Tn2/, Tn7, Tn3 and Tn9 could be detected in some isolates. In addition, R-genes, integron and transposon sequences were carried by conjugative plasmids in 12 of the 13 isolates. Genomic typing with RAPD and JuM-PFGE macrorestriction discriminated the isolates, which proved to be highly heterogeneous, into 5 RAPD and 11 JfcM-profiles.

Originalsprog	Engelsk
Titel	Modern Multidisciplinary Applied Microbiology : Exploiting Microbes and Their Interactions
Antal sider	7
Forlag	Wiley-VCH
Publikationsdato	2006
Sider	685-691
ISBN (Trykt)	3527316116, 9783527316113
DOI	https://doi.org/10.1002/9783527611904.ch123
Status	Udgivet - 2006
Udgivet eksternt	Ja

Adgang til dokumentet

10.1002/9783527611904.ch123

Citationsformater

Rodríguez, I., Herrero, A., Martí, N., Martín, M. C., Rodicio, M. R., & Mendoza, M. C. (2006). Detection and Characterization of Integrons, Transposons, Plasmids and Genomic types in Multidrug Resistant Clinical Isolates of Salmonella enterica Serovar Virchow. I Modern Multidisciplinary Applied Microbiology: Exploiting Microbes and Their Interactions (s. 685-691). Wiley-VCH. https://doi.org/10.1002/9783527611904.ch123

Detection and Characterization of Integrons, Transposons, Plasmids and Genomic types in Multidrug Resistant Clinical Isolates of Salmonella enterica Serovar Virchow. / Rodríguez, I.; Herrero, A.; Martí, N. et al.
Modern Multidisciplinary Applied Microbiology: Exploiting Microbes and Their Interactions. Wiley-VCH, 2006. s. 685-691.

Publikation: Bidrag til bog/antologi/rapport › Bidrag til bog/antologi › Forskning › peer review

Rodríguez, I, Herrero, A, Martí, N, Martín, MC, Rodicio, MR & Mendoza, MC 2006, Detection and Characterization of Integrons, Transposons, Plasmids and Genomic types in Multidrug Resistant Clinical Isolates of Salmonella enterica Serovar Virchow. i Modern Multidisciplinary Applied Microbiology: Exploiting Microbes and Their Interactions. Wiley-VCH, s. 685-691. https://doi.org/10.1002/9783527611904.ch123

Rodríguez I, Herrero A, Martí N, Martín MC, Rodicio MR, Mendoza MC. Detection and Characterization of Integrons, Transposons, Plasmids and Genomic types in Multidrug Resistant Clinical Isolates of Salmonella enterica Serovar Virchow. I Modern Multidisciplinary Applied Microbiology: Exploiting Microbes and Their Interactions. Wiley-VCH. 2006. s. 685-691 doi: 10.1002/9783527611904.ch123

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abstract = "Thirteen multidrug resistant (to 7 or more antimicrobial drugs) isolates of Salmonella enterica sv Virchow were analyzed by different molecular approaches. PCR amplification allowed the detection of several resistance (R) determinants and of integron and transposon sequences. The isolates were grouped into 11 resistance genotypes and 4 integron profiles. All of them were positive for class 1 integrons (with 3 different arrays of gene cassettes in the variable regions), and three isolates also contained the same class 2 integron. Specific sequences of transposons Tn2/, Tn7, Tn3 and Tn9 could be detected in some isolates. In addition, R-genes, integron and transposon sequences were carried by conjugative plasmids in 12 of the 13 isolates. Genomic typing with RAPD and JuM-PFGE macrorestriction discriminated the isolates, which proved to be highly heterogeneous, into 5 RAPD and 11 JfcM-profiles.",

keywords = "Antimicrobial resistance, Integrons, PFGE, Plasmids, Salmonella, Transposons",

author = "I. Rodr{\'i}guez and A. Herrero and N. Mart{\'i} and Mart{\'i}n, {M. C.} and Rodicio, {M. R.} and Mendoza, {M. C.}",

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doi = "10.1002/9783527611904.ch123",

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AU - Rodríguez, I.

AU - Herrero, A.

AU - Martí, N.

AU - Martín, M. C.

AU - Rodicio, M. R.

AU - Mendoza, M. C.

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N2 - Thirteen multidrug resistant (to 7 or more antimicrobial drugs) isolates of Salmonella enterica sv Virchow were analyzed by different molecular approaches. PCR amplification allowed the detection of several resistance (R) determinants and of integron and transposon sequences. The isolates were grouped into 11 resistance genotypes and 4 integron profiles. All of them were positive for class 1 integrons (with 3 different arrays of gene cassettes in the variable regions), and three isolates also contained the same class 2 integron. Specific sequences of transposons Tn2/, Tn7, Tn3 and Tn9 could be detected in some isolates. In addition, R-genes, integron and transposon sequences were carried by conjugative plasmids in 12 of the 13 isolates. Genomic typing with RAPD and JuM-PFGE macrorestriction discriminated the isolates, which proved to be highly heterogeneous, into 5 RAPD and 11 JfcM-profiles.

AB - Thirteen multidrug resistant (to 7 or more antimicrobial drugs) isolates of Salmonella enterica sv Virchow were analyzed by different molecular approaches. PCR amplification allowed the detection of several resistance (R) determinants and of integron and transposon sequences. The isolates were grouped into 11 resistance genotypes and 4 integron profiles. All of them were positive for class 1 integrons (with 3 different arrays of gene cassettes in the variable regions), and three isolates also contained the same class 2 integron. Specific sequences of transposons Tn2/, Tn7, Tn3 and Tn9 could be detected in some isolates. In addition, R-genes, integron and transposon sequences were carried by conjugative plasmids in 12 of the 13 isolates. Genomic typing with RAPD and JuM-PFGE macrorestriction discriminated the isolates, which proved to be highly heterogeneous, into 5 RAPD and 11 JfcM-profiles.

KW - Antimicrobial resistance

KW - Integrons

KW - PFGE

KW - Plasmids

KW - Salmonella

KW - Transposons

U2 - 10.1002/9783527611904.ch123

DO - 10.1002/9783527611904.ch123

M3 - Book chapter

AN - SCOPUS:84992231754

SN - 3527316116

SN - 9783527316113

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EP - 691

BT - Modern Multidisciplinary Applied Microbiology

PB - Wiley-VCH

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