Abstract
| Originalsprog | Engelsk |
|---|---|
| Tidsskrift | Prenatal Diagnosis |
| Vol/bind | 19 |
| Udgave nummer | 3 |
| Sider (fra-til) | 271-278 |
| Antal sider | 8 |
| ISSN | 0197-3851 |
| Status | Udgivet - 1999 |
Bibliografisk note
Keywords: Antibodies, Monoclonal; DNA; Fathers; Female; Genome, Human; Humans; Immunomagnetic Separation; Male; Maternal-Fetal Exchange; Models, Genetic; Pregnancy; Prenatal Diagnosis; Tandem Repeat Sequences; Trophoblasts; Y ChromosomeCitationsformater
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I: Prenatal Diagnosis, Bind 19, Nr. 3, 1999, s. 271-278.
Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › peer review
}
TY - JOUR
T1 - Detection of fetal-specific DNA after enrichment for trophoblasts using the monoclonal antibody LK26 in model systems but failure to demonstrate fetal DNA in maternal peripheral blood
AU - Hviid, T V
AU - Sørensen, S
AU - Morling, N
N1 - Keywords: Antibodies, Monoclonal; DNA; Fathers; Female; Genome, Human; Humans; Immunomagnetic Separation; Male; Maternal-Fetal Exchange; Models, Genetic; Pregnancy; Prenatal Diagnosis; Tandem Repeat Sequences; Trophoblasts; Y Chromosome
PY - 1999
Y1 - 1999
N2 - Trophoblast cells can be detected in maternal blood during normal human pregnancy and DNA from these cells may be used for non-invasive prenatal diagnosis of inherited diseases. The possibility of enriching trophoblast cells from maternal blood samples using a monoclonal antibody (LK26) against a folate-binding protein, which recognizes trophoblast in normal tissues, in conjunction with immunomagnetic cell sorting was investigated. Verification of the presence of fetal DNA in the sorted samples was done by detection of fetal/paternal-specific short tandem repeat (STR) alleles using polymerase chain reaction (PCR) and automated fluorescence-based genotyping. After successful initial experiments using retroplacental blood samples with a high number of trophoblast cells or an artificial mixture of trophoblast cells and blood, several versions of the enrichment method were attempted on peripheral maternal blood samples. However, it was not possible to detect fetal DNA sequences in these samples, most probably due to the extremely low number of trophoblast cells. Positive identification and retrieval of trophoblast cells in suspension or trophoblast nuclear material prepared on microscope slides after cell sorting procedures can be a solution to this problem.
AB - Trophoblast cells can be detected in maternal blood during normal human pregnancy and DNA from these cells may be used for non-invasive prenatal diagnosis of inherited diseases. The possibility of enriching trophoblast cells from maternal blood samples using a monoclonal antibody (LK26) against a folate-binding protein, which recognizes trophoblast in normal tissues, in conjunction with immunomagnetic cell sorting was investigated. Verification of the presence of fetal DNA in the sorted samples was done by detection of fetal/paternal-specific short tandem repeat (STR) alleles using polymerase chain reaction (PCR) and automated fluorescence-based genotyping. After successful initial experiments using retroplacental blood samples with a high number of trophoblast cells or an artificial mixture of trophoblast cells and blood, several versions of the enrichment method were attempted on peripheral maternal blood samples. However, it was not possible to detect fetal DNA sequences in these samples, most probably due to the extremely low number of trophoblast cells. Positive identification and retrieval of trophoblast cells in suspension or trophoblast nuclear material prepared on microscope slides after cell sorting procedures can be a solution to this problem.
M3 - Journal article
C2 - 10210130
SN - 0197-3851
VL - 19
SP - 271
EP - 278
JO - Prenatal Diagnosis
JF - Prenatal Diagnosis
IS - 3
ER -