Determination of membrane protein orientation upon liposomal reconstitution down to the single vesicle level

Sarina Veit, Laura Charlotte Paweletz, Thomas Guenther Pomorski*

*Corresponding author af dette arbejde

Publikation: Bidrag til tidsskriftReviewForskningpeer review

1 Citationer (Scopus)
7 Downloads (Pure)

Abstract

Reconstitution of membrane proteins into liposomal membranes represents a key technique in enabling functional analysis under well-defined conditions. In this review, we provide a brief introduction to selected methods that have been developed to determine membrane protein orientation after reconstitution in liposomes, including approaches based on proteolytic digestion with proteases, site-specific labeling, fluorescence quenching and activity assays. In addition, we briefly highlight new strategies based on single vesicle analysis to address the problem of sample heterogeneity.

OriginalsprogEngelsk
TidsskriftBiological Chemistry
Vol/bind404
Udgave nummer7
ISSN1431-6730
DOI
StatusUdgivet - 2023

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