Abstract
Reconstitution of membrane proteins into liposomal membranes represents a key technique in enabling functional analysis under well-defined conditions. In this review, we provide a brief introduction to selected methods that have been developed to determine membrane protein orientation after reconstitution in liposomes, including approaches based on proteolytic digestion with proteases, site-specific labeling, fluorescence quenching and activity assays. In addition, we briefly highlight new strategies based on single vesicle analysis to address the problem of sample heterogeneity.
Originalsprog | Engelsk |
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Tidsskrift | Biological Chemistry |
Vol/bind | 404 |
Udgave nummer | 7 |
ISSN | 1431-6730 |
DOI | |
Status | Udgivet - 2023 |