TY - JOUR
T1 - Determination of psilocybin in Psilocybe semilanceata by capillary zone electrophoresis
AU - Pedersen-Bjergaard, Stig
AU - Sannes, Ellen
AU - Rasmussen, Knut Einar
AU - Tønnesen, Finn
PY - 1997/7/4
Y1 - 1997/7/4
N2 - A capillary zone electrophoretic (CZE) method was developed for the rapid determination of psilocybin in Psilocybe semilanceata. Following a simple two step extraction with 3.0+2.0 ml methanol, the hallucinogenic compound was effectively separated from matrix components by CZE utilizing a 10 mM borate-phosphate running buffer adjusted to pH 11.5. The identity of psilocybin was confirmed by migration time information and by W spectra, while quantitation was accomplished utilizing barbital as internal standard. The calibration curve for psilocybin was linear within 0.01-1 mg/ml, while intra-day and inter-day variations of quantitative data were 0.5 and 2.5% R.S.D., respectively. In addition to psilocybin, the method was also suitable for the determination of the structurally related compound baeocystin.
AB - A capillary zone electrophoretic (CZE) method was developed for the rapid determination of psilocybin in Psilocybe semilanceata. Following a simple two step extraction with 3.0+2.0 ml methanol, the hallucinogenic compound was effectively separated from matrix components by CZE utilizing a 10 mM borate-phosphate running buffer adjusted to pH 11.5. The identity of psilocybin was confirmed by migration time information and by W spectra, while quantitation was accomplished utilizing barbital as internal standard. The calibration curve for psilocybin was linear within 0.01-1 mg/ml, while intra-day and inter-day variations of quantitative data were 0.5 and 2.5% R.S.D., respectively. In addition to psilocybin, the method was also suitable for the determination of the structurally related compound baeocystin.
KW - Psilocybin
UR - http://www.scopus.com/inward/record.url?scp=0030764574&partnerID=8YFLogxK
U2 - 10.1016/S0378-4347(97)00127-8
DO - 10.1016/S0378-4347(97)00127-8
M3 - Journal article
C2 - 9252052
AN - SCOPUS:0030764574
VL - 694
SP - 375
EP - 381
JO - Journal of Chromatography - Biomedical Applications
JF - Journal of Chromatography - Biomedical Applications
SN - 0378-4347
IS - 2
ER -