@inbook{599f285cfeb1479ca0d26c2ea2063397,
title = "Development and Characterization of Flavin-Binding Fluorescent Proteins, Part II: Advanced Characterization",
abstract = "Flavin-based fluorescent proteins (FbFPs), a class of small fluorescent proteins derived from light-oxygen-voltage (LOV) domains, bind ubiquitous endogenous flavins as chromophores. Due to their unique properties, they can be used as versatile in vivo reporter proteins under aerobic and anaerobic conditions. This chapter presents methodologies for in-depth characterization of the biochemical, spectroscopic, photophysical, and photochemical properties of FbFPs.",
keywords = "Flavin, Flavin-binding fluorescent protein, Fluorescence brightness, Fluorescence lifetime, Fluorescence microscopy, Genetically encoded photosensitizer, LOV domain, Phototoxicity",
author = "Bitzenhofer, {Nora Lisa} and Fabienne Hilgers and Bosio, {Gabriela N.} and Joaquim Torra and Giorgia Casini and Beinlich, {Felix R.M.} and Esther Knieps-Gr{\"u}nhagen and Valentin Gordeliy and Jaeger, {Karl Erich} and Santi Nonell and Ulrich Krauss and Thomas Gensch and Thomas Drepper",
note = "Funding Information: N.L.B., F.H., G.B., and J.T. contributed equally to the preparation of the chapter. S.N., U.K., T.G., and T.D. are co-corresponding authors. The PS characterization was supported by grants from the Bioeconomy Science Center awarded to F.H. The scientific activities of the Bioeconomy Science Center were financially supported by the Ministry of Innovation, Science and Research of the German federal state of North Rhine-Westphalia MIWF within the frame-work of the NRW Strategieprojekt BioSC (No. 313/323-400-00213). The authors thank Ivan Gushchin for his contributions to writing and editing of this chapter. Publisher Copyright: {\textcopyright} 2023, The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.",
year = "2023",
doi = "10.1007/978-1-0716-2667-2_7",
language = "English",
isbn = "978-1-0716-2666-5",
volume = "2564",
series = "Methods in Molecular Biology",
publisher = "Humana Press",
pages = "143--183",
booktitle = "Methods in Molecular Biology",
address = "United States",
}