Dicer is required for chromosome segregation and gene silencing in fission yeast cells.

Patrick Provost; Rebecca A Silverstein; David Dishart; Julian Osmond A Valfridsson; Ingela Djupedal; Barbara Kniola; Anthony Wright; Bengt Samuelsson; Olof Radmark; Karl Ekwall

doi:10.1073/pnas.212633199

Dicer is required for chromosome segregation and gene silencing in fission yeast cells.

Patrick Provost, Rebecca A Silverstein, David Dishart, Julian Osmond A Valfridsson, Ingela Djupedal, Barbara Kniola, Anthony Wright, Bengt Samuelsson, Olof Radmark, Karl Ekwall

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › peer review

111 Citationer (Scopus)

Abstract

Udgivelsesdato: 2002-Dec-24

Originalsprog	Engelsk
Tidsskrift	Proceedings of the National Academy of Science of the United States of America
Vol/bind	99
Udgave nummer	26
Sider (fra-til)	16648-53
Antal sider	5
ISSN	0027-8424
DOI	https://doi.org/10.1073/pnas.212633199
Status	Udgivet - 2002
Udgivet eksternt	Ja

Bibliografisk note

Keywords: Chromosome Segregation; Endoribonucleases; Gene Silencing; Humans; Reverse Transcriptase Polymerase Chain Reaction; Ribonuclease III; Schizosaccharomyces

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10.1073/pnas.212633199

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Citationsformater

Provost, P., Silverstein, R. A., Dishart, D., Valfridsson, J. O. A., Djupedal, I., Kniola, B., Wright, A., Samuelsson, B., Radmark, O., & Ekwall, K. (2002). Dicer is required for chromosome segregation and gene silencing in fission yeast cells. Proceedings of the National Academy of Science of the United States of America, 99(26), 16648-53. https://doi.org/10.1073/pnas.212633199

Provost, P, Silverstein, RA, Dishart, D, Valfridsson, JOA, Djupedal, I, Kniola, B, Wright, A, Samuelsson, B, Radmark, O & Ekwall, K 2002, 'Dicer is required for chromosome segregation and gene silencing in fission yeast cells.', Proceedings of the National Academy of Science of the United States of America, bind 99, nr. 26, s. 16648-53. https://doi.org/10.1073/pnas.212633199

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title = "Dicer is required for chromosome segregation and gene silencing in fission yeast cells.",

abstract = "RNA interference is a form of gene silencing in which the nuclease Dicer cleaves double-stranded RNA into small interfering RNAs. Here we report a role for Dicer in chromosome segregation of fission yeast. Deletion of the Dicer (dcr1+) gene caused slow growth, sensitivity to thiabendazole, lagging chromosomes during anaphase, and abrogated silencing of centromeric repeats. As Dicer in other species, Dcr1p degraded double-stranded RNA into approximately 23 nucleotide fragments in vitro, and dcr1Delta cells were partially rescued by expression of human Dicer, indicating evolutionarily conserved functions. Expression profiling demonstrated that dcr1+ was required for silencing of two genes containing a conserved motif.",

author = "Patrick Provost and Silverstein, {Rebecca A} and David Dishart and Valfridsson, {Julian Osmond A} and Ingela Djupedal and Barbara Kniola and Anthony Wright and Bengt Samuelsson and Olof Radmark and Karl Ekwall",

note = "Keywords: Chromosome Segregation; Endoribonucleases; Gene Silencing; Humans; Reverse Transcriptase Polymerase Chain Reaction; Ribonuclease III; Schizosaccharomyces",

year = "2002",

doi = "10.1073/pnas.212633199",

language = "English",

volume = "99",

pages = "16648--53",

journal = "Proceedings of the National Academy of Science of the United States of America",

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TY - JOUR

T1 - Dicer is required for chromosome segregation and gene silencing in fission yeast cells.

AU - Provost, Patrick

AU - Silverstein, Rebecca A

AU - Dishart, David

AU - Valfridsson, Julian Osmond A

AU - Djupedal, Ingela

AU - Kniola, Barbara

AU - Wright, Anthony

AU - Samuelsson, Bengt

AU - Radmark, Olof

AU - Ekwall, Karl

N1 - Keywords: Chromosome Segregation; Endoribonucleases; Gene Silencing; Humans; Reverse Transcriptase Polymerase Chain Reaction; Ribonuclease III; Schizosaccharomyces

PY - 2002

Y1 - 2002

N2 - RNA interference is a form of gene silencing in which the nuclease Dicer cleaves double-stranded RNA into small interfering RNAs. Here we report a role for Dicer in chromosome segregation of fission yeast. Deletion of the Dicer (dcr1+) gene caused slow growth, sensitivity to thiabendazole, lagging chromosomes during anaphase, and abrogated silencing of centromeric repeats. As Dicer in other species, Dcr1p degraded double-stranded RNA into approximately 23 nucleotide fragments in vitro, and dcr1Delta cells were partially rescued by expression of human Dicer, indicating evolutionarily conserved functions. Expression profiling demonstrated that dcr1+ was required for silencing of two genes containing a conserved motif.

AB - RNA interference is a form of gene silencing in which the nuclease Dicer cleaves double-stranded RNA into small interfering RNAs. Here we report a role for Dicer in chromosome segregation of fission yeast. Deletion of the Dicer (dcr1+) gene caused slow growth, sensitivity to thiabendazole, lagging chromosomes during anaphase, and abrogated silencing of centromeric repeats. As Dicer in other species, Dcr1p degraded double-stranded RNA into approximately 23 nucleotide fragments in vitro, and dcr1Delta cells were partially rescued by expression of human Dicer, indicating evolutionarily conserved functions. Expression profiling demonstrated that dcr1+ was required for silencing of two genes containing a conserved motif.

U2 - 10.1073/pnas.212633199

DO - 10.1073/pnas.212633199

M3 - Journal article

C2 - 12482946

SN - 0027-8424

VL - 99

SP - 16648

EP - 16653

JO - Proceedings of the National Academy of Science of the United States of America

JF - Proceedings of the National Academy of Science of the United States of America

IS - 26

ER -