Dichotomy of the T cell response to Leishmania antigens in patients suffering from cutaneous leishmaniasis; absence or scarcity of Th1 activity is associated with severe infections

A Gaafar; A Kharazmi; A Ismail; M Kemp; A Hey; C B Christensen; M Dafalla; A Y el Kadaro; A M el Hassan; T G Theander

Dichotomy of the T cell response to Leishmania antigens in patients suffering from cutaneous leishmaniasis; absence or scarcity of Th1 activity is associated with severe infections

A Gaafar, A Kharazmi, A Ismail, M Kemp, A Hey, C B Christensen, M Dafalla, A Y el Kadaro, A M el Hassan, T G Theander

Centre for Medical Parasitology

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › peer review

54 Citationer (Scopus)

Abstract

Udgivelsesdato: 1995-May

Originalsprog	Engelsk
Tidsskrift	Clinical and Experimental Immunology
Vol/bind	100
Udgave nummer	2
Sider (fra-til)	239-45
Antal sider	6
ISSN	0009-9104
Status	Udgivet - 1995

Bibliografisk note

Keywords: Animals; Antigens, Protozoan; Interferon Type II; Interleukin-4; Leishmania major; Leishmaniasis, Cutaneous; Lymphocyte Activation; T-Lymphocytes; Th1 Cells; Th2 Cells

Citationsformater

Gaafar, A., Kharazmi, A., Ismail, A., Kemp, M., Hey, A., Christensen, C. B., Dafalla, M., el Kadaro, A. Y., el Hassan, A. M., & Theander, T. G. (1995). Dichotomy of the T cell response to Leishmania antigens in patients suffering from cutaneous leishmaniasis; absence or scarcity of Th1 activity is associated with severe infections. Clinical and Experimental Immunology, 100(2), 239-45.

Dichotomy of the T cell response to Leishmania antigens in patients suffering from cutaneous leishmaniasis; absence or scarcity of Th1 activity is associated with severe infections. / Gaafar, A; Kharazmi, A; Ismail, A et al.
I: Clinical and Experimental Immunology, Bind 100, Nr. 2, 1995, s. 239-45.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › peer review

Gaafar, A, Kharazmi, A, Ismail, A, Kemp, M, Hey, A, Christensen, CB, Dafalla, M, el Kadaro, AY, el Hassan, AM & Theander, TG 1995, 'Dichotomy of the T cell response to Leishmania antigens in patients suffering from cutaneous leishmaniasis; absence or scarcity of Th1 activity is associated with severe infections', Clinical and Experimental Immunology, bind 100, nr. 2, s. 239-45.

@article{f569c540a0d911dd86a6000ea68e967b,

title = "Dichotomy of the T cell response to Leishmania antigens in patients suffering from cutaneous leishmaniasis; absence or scarcity of Th1 activity is associated with severe infections",

abstract = "The T cell response was studied in 25 patients suffering from cutaneous leishmaniasis caused by Leishmania major with severe (n = 10) and mild (n = 15) disease manifestations. Peripheral blood mononuclear cells (PBMC) from the patients were activated by sonicates of Leishmania promastigotes (LMP) and amastigotes (LDA), and the surface protease gp63. The proliferative responses to Leishmania antigens were lower in patients with severe disease than in patients with mild disease (P = 0.01-0.05), and such a difference was not observed in the response to purified protein derivative of tuberculin (PPD) or tetanus toxoid (TT). LMP-induced interferon-gamma (IFN-gamma) production was lower in patients with severe than in patients with mild disease (P < 0.05). When the IL-4 and IFN-gamma responses of each patient were considered, two response patterns were observed in the cultures activated by the Leishmania sonicates. One response pattern was characterized by high production of IFN-gamma without production of IL-4 (a Th1-like pattern), the other was characterized by low IFN-gamma levels which in most cases were associated with IL-4 production (not a Th1-like pattern). These patterns could not be distinguished when the cells from the same donors were stimulated by TT and PPD. The percentages of patients with a Th1-like response pattern after stimulation by LMP in patients with severe and mild disease manifestations were 30% and 80%, respectively. This difference was statistically significant (P = 0.034).",

author = "A Gaafar and A Kharazmi and A Ismail and M Kemp and A Hey and Christensen, {C B} and M Dafalla and {el Kadaro}, {A Y} and {el Hassan}, {A M} and Theander, {T G}",

note = "Keywords: Animals; Antigens, Protozoan; Interferon Type II; Interleukin-4; Leishmania major; Leishmaniasis, Cutaneous; Lymphocyte Activation; T-Lymphocytes; Th1 Cells; Th2 Cells",

year = "1995",

language = "English",

volume = "100",

pages = "239--45",

journal = "Clinical and Experimental Immunology",

issn = "0009-9104",

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TY - JOUR

T1 - Dichotomy of the T cell response to Leishmania antigens in patients suffering from cutaneous leishmaniasis; absence or scarcity of Th1 activity is associated with severe infections

AU - Gaafar, A

AU - Kharazmi, A

AU - Ismail, A

AU - Kemp, M

AU - Hey, A

AU - Christensen, C B

AU - Dafalla, M

AU - el Kadaro, A Y

AU - el Hassan, A M

AU - Theander, T G

N1 - Keywords: Animals; Antigens, Protozoan; Interferon Type II; Interleukin-4; Leishmania major; Leishmaniasis, Cutaneous; Lymphocyte Activation; T-Lymphocytes; Th1 Cells; Th2 Cells

PY - 1995

Y1 - 1995

N2 - The T cell response was studied in 25 patients suffering from cutaneous leishmaniasis caused by Leishmania major with severe (n = 10) and mild (n = 15) disease manifestations. Peripheral blood mononuclear cells (PBMC) from the patients were activated by sonicates of Leishmania promastigotes (LMP) and amastigotes (LDA), and the surface protease gp63. The proliferative responses to Leishmania antigens were lower in patients with severe disease than in patients with mild disease (P = 0.01-0.05), and such a difference was not observed in the response to purified protein derivative of tuberculin (PPD) or tetanus toxoid (TT). LMP-induced interferon-gamma (IFN-gamma) production was lower in patients with severe than in patients with mild disease (P < 0.05). When the IL-4 and IFN-gamma responses of each patient were considered, two response patterns were observed in the cultures activated by the Leishmania sonicates. One response pattern was characterized by high production of IFN-gamma without production of IL-4 (a Th1-like pattern), the other was characterized by low IFN-gamma levels which in most cases were associated with IL-4 production (not a Th1-like pattern). These patterns could not be distinguished when the cells from the same donors were stimulated by TT and PPD. The percentages of patients with a Th1-like response pattern after stimulation by LMP in patients with severe and mild disease manifestations were 30% and 80%, respectively. This difference was statistically significant (P = 0.034).

AB - The T cell response was studied in 25 patients suffering from cutaneous leishmaniasis caused by Leishmania major with severe (n = 10) and mild (n = 15) disease manifestations. Peripheral blood mononuclear cells (PBMC) from the patients were activated by sonicates of Leishmania promastigotes (LMP) and amastigotes (LDA), and the surface protease gp63. The proliferative responses to Leishmania antigens were lower in patients with severe disease than in patients with mild disease (P = 0.01-0.05), and such a difference was not observed in the response to purified protein derivative of tuberculin (PPD) or tetanus toxoid (TT). LMP-induced interferon-gamma (IFN-gamma) production was lower in patients with severe than in patients with mild disease (P < 0.05). When the IL-4 and IFN-gamma responses of each patient were considered, two response patterns were observed in the cultures activated by the Leishmania sonicates. One response pattern was characterized by high production of IFN-gamma without production of IL-4 (a Th1-like pattern), the other was characterized by low IFN-gamma levels which in most cases were associated with IL-4 production (not a Th1-like pattern). These patterns could not be distinguished when the cells from the same donors were stimulated by TT and PPD. The percentages of patients with a Th1-like response pattern after stimulation by LMP in patients with severe and mild disease manifestations were 30% and 80%, respectively. This difference was statistically significant (P = 0.034).

M3 - Journal article

C2 - 7743662

SN - 0009-9104

VL - 100

SP - 239

EP - 245

JO - Clinical and Experimental Immunology

JF - Clinical and Experimental Immunology

IS - 2

ER -