Diesel exhaust particles are mutagenic in FE1-MutaMouse lung epithelial cells

Nicklas Raun Jacobsen; Peter Møller; Corey Alexander Cohn; Steffen Loft; Ulla Vogel; Håkan Wallin

doi:10.1016/j.mrfmmm.2008.03.001

Diesel exhaust particles are mutagenic in FE1-MutaMouse lung epithelial cells

Nicklas Raun Jacobsen, Peter Møller, Corey Alexander Cohn, Steffen Loft, Ulla Vogel, Håkan Wallin

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › peer review

57 Citationer (Scopus)

Abstract

Udgivelsesdato: 2008-May-10

Originalsprog	Engelsk
Tidsskrift	Mutation Research - Reviews
Vol/bind	641
Udgave nummer	1-2
Sider (fra-til)	54-7
Antal sider	3
ISSN	1383-5742
DOI	https://doi.org/10.1016/j.mrfmmm.2008.03.001
Status	Udgivet - 2008

Bibliografisk note

Keywords: Air Pollutants; Animals; Cells, Cultured; Epithelial Cells; Lung; Mice; Mutagenesis; Mutagens; Reactive Oxygen Species; Soot; Vehicle Emissions

Adgang til dokumentet

10.1016/j.mrfmmm.2008.03.001

Citationsformater

@article{416e7780e93511ddbf70000ea68e967b,

title = "Diesel exhaust particles are mutagenic in FE1-MutaMouse lung epithelial cells",

abstract = "The particulate phase of diesel engine exhaust is likely carcinogenic. However, the mechanisms of diesel exhaust particles (DEPs) induced mutagenicity/carcinogenicity are still largely unknown. We determined the mutant frequency following eight repeated 72 h incubations with 37.5 or 75 microg/ml DEP (NIST SRM 1650) in the FE1-MutaMouse lung epithelial cell line. We measured DEP-induced acellular and intracellular production of reactive oxygen species (ROS) and compared with ROS production induced by carbon black, which we have previously shown is mutagenic in this cell line [N.R. Jacobsen, A.T. Saber, P. White, P. Moller, G. Pojana, U. Vogel, S. Loft, J. Gingerich, L. Soper, G.R. Douglas, H. Wallin. Increased mutant frequency by carbon black, but not quartz, in the lacZ and cII transgenes of mutamouse lung epithelial cells, Environ. Mol. Mutagen. 48(6) (2007) 451-461]. The mutant frequency was marginally elevated in cells treated with 37.5 microg/ml DEP (1.29-fold [95% CI: 0.96-1.60], p=0.08) and significantly increased in cells treated with 75 microg/ml DEP (1.55-fold [95% CI: 1.23-1.87], p < 0.001). ROS production from DEP was low both within cells and in acellular systems when compared to carbon black. These results show that DEP are mutagenic in a mammalian cell line in vitro and that additional pathways besides ROS production, such as those involving the presence of polycyclic aromatic hydrocarbons, likely are involved in the mutagenesis.",

author = "Jacobsen, {Nicklas Raun} and Peter M{\o}ller and Cohn, {Corey Alexander} and Steffen Loft and Ulla Vogel and H{\aa}kan Wallin",

note = "Keywords: Air Pollutants; Animals; Cells, Cultured; Epithelial Cells; Lung; Mice; Mutagenesis; Mutagens; Reactive Oxygen Species; Soot; Vehicle Emissions",

year = "2008",

doi = "10.1016/j.mrfmmm.2008.03.001",

language = "English",

volume = "641",

pages = "54--7",

journal = "Mutation Research - Reviews",

issn = "1383-5742",

publisher = "Elsevier",

number = "1-2",

}

TY - JOUR

T1 - Diesel exhaust particles are mutagenic in FE1-MutaMouse lung epithelial cells

AU - Jacobsen, Nicklas Raun

AU - Møller, Peter

AU - Cohn, Corey Alexander

AU - Loft, Steffen

AU - Vogel, Ulla

AU - Wallin, Håkan

N1 - Keywords: Air Pollutants; Animals; Cells, Cultured; Epithelial Cells; Lung; Mice; Mutagenesis; Mutagens; Reactive Oxygen Species; Soot; Vehicle Emissions

PY - 2008

Y1 - 2008

N2 - The particulate phase of diesel engine exhaust is likely carcinogenic. However, the mechanisms of diesel exhaust particles (DEPs) induced mutagenicity/carcinogenicity are still largely unknown. We determined the mutant frequency following eight repeated 72 h incubations with 37.5 or 75 microg/ml DEP (NIST SRM 1650) in the FE1-MutaMouse lung epithelial cell line. We measured DEP-induced acellular and intracellular production of reactive oxygen species (ROS) and compared with ROS production induced by carbon black, which we have previously shown is mutagenic in this cell line [N.R. Jacobsen, A.T. Saber, P. White, P. Moller, G. Pojana, U. Vogel, S. Loft, J. Gingerich, L. Soper, G.R. Douglas, H. Wallin. Increased mutant frequency by carbon black, but not quartz, in the lacZ and cII transgenes of mutamouse lung epithelial cells, Environ. Mol. Mutagen. 48(6) (2007) 451-461]. The mutant frequency was marginally elevated in cells treated with 37.5 microg/ml DEP (1.29-fold [95% CI: 0.96-1.60], p=0.08) and significantly increased in cells treated with 75 microg/ml DEP (1.55-fold [95% CI: 1.23-1.87], p < 0.001). ROS production from DEP was low both within cells and in acellular systems when compared to carbon black. These results show that DEP are mutagenic in a mammalian cell line in vitro and that additional pathways besides ROS production, such as those involving the presence of polycyclic aromatic hydrocarbons, likely are involved in the mutagenesis.

AB - The particulate phase of diesel engine exhaust is likely carcinogenic. However, the mechanisms of diesel exhaust particles (DEPs) induced mutagenicity/carcinogenicity are still largely unknown. We determined the mutant frequency following eight repeated 72 h incubations with 37.5 or 75 microg/ml DEP (NIST SRM 1650) in the FE1-MutaMouse lung epithelial cell line. We measured DEP-induced acellular and intracellular production of reactive oxygen species (ROS) and compared with ROS production induced by carbon black, which we have previously shown is mutagenic in this cell line [N.R. Jacobsen, A.T. Saber, P. White, P. Moller, G. Pojana, U. Vogel, S. Loft, J. Gingerich, L. Soper, G.R. Douglas, H. Wallin. Increased mutant frequency by carbon black, but not quartz, in the lacZ and cII transgenes of mutamouse lung epithelial cells, Environ. Mol. Mutagen. 48(6) (2007) 451-461]. The mutant frequency was marginally elevated in cells treated with 37.5 microg/ml DEP (1.29-fold [95% CI: 0.96-1.60], p=0.08) and significantly increased in cells treated with 75 microg/ml DEP (1.55-fold [95% CI: 1.23-1.87], p < 0.001). ROS production from DEP was low both within cells and in acellular systems when compared to carbon black. These results show that DEP are mutagenic in a mammalian cell line in vitro and that additional pathways besides ROS production, such as those involving the presence of polycyclic aromatic hydrocarbons, likely are involved in the mutagenesis.

U2 - 10.1016/j.mrfmmm.2008.03.001

DO - 10.1016/j.mrfmmm.2008.03.001

M3 - Journal article

C2 - 18423769

VL - 641

SP - 54

EP - 57

JO - Mutation Research - Reviews

JF - Mutation Research - Reviews

SN - 1383-5742

IS - 1-2

ER -