Abstract
| Originalsprog | Engelsk |
|---|---|
| Tidsskrift | American Journal of Physiology: Endocrinology and Metabolism |
| Vol/bind | 290 |
| Udgave nummer | 5 |
| Sider (fra-til) | E961-7 |
| Antal sider | 7 |
| ISSN | 0193-1849 |
| DOI | |
| Status | Udgivet - 2006 |
Bibliografisk note
Keywords: Adiponectin; Adipose Tissue; Antigens, CD; Antigens, CD14; Antigens, Differentiation, Myelomonocytic; Blood Pressure; Body Weight; C-Reactive Protein; Chemokine CCL2; Diet; Exercise; Female; Gene Expression; Glucose Tolerance Test; Humans; Inflammation; Interleukin-6; Interleukin-8; Macrophages; Male; Muscle, Skeletal; Obesity; Receptors, Adiponectin; Receptors, Cell Surface; Tumor Necrosis Factor-alphaAdgang til dokumentet
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Diet and exercise reduce low-grade inflammation and macrophage infiltration in adipose tissue but not in skeletal muscle in severely obese subjects. / Bruun, Jens M; Helge, Jørn W; Richelsen, Bjørn; Stallknecht, Bente.
I: American Journal of Physiology: Endocrinology and Metabolism, Bind 290, Nr. 5, 2006, s. E961-7.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › peer review
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TY - JOUR
T1 - Diet and exercise reduce low-grade inflammation and macrophage infiltration in adipose tissue but not in skeletal muscle in severely obese subjects
AU - Bruun, Jens M
AU - Helge, Jørn W
AU - Richelsen, Bjørn
AU - Stallknecht, Bente
N1 - Keywords: Adiponectin; Adipose Tissue; Antigens, CD; Antigens, CD14; Antigens, Differentiation, Myelomonocytic; Blood Pressure; Body Weight; C-Reactive Protein; Chemokine CCL2; Diet; Exercise; Female; Gene Expression; Glucose Tolerance Test; Humans; Inflammation; Interleukin-6; Interleukin-8; Macrophages; Male; Muscle, Skeletal; Obesity; Receptors, Adiponectin; Receptors, Cell Surface; Tumor Necrosis Factor-alpha
PY - 2006
Y1 - 2006
N2 - Obesity is associated with low-grade inflammation, insulin resistance, type 2 diabetes, and cardiovascular disease. This study investigated the effect of a 15-wk lifestyle intervention (hypocaloric diet and daily exercise) on inflammatory markers in plasma, adipose tissue (AT), and skeletal muscle (SM) in 27 severely obese subjects (mean body mass index: 45.8 kg/m2). Plasma samples, subcutaneous abdominal AT biopsies, and vastus lateralis SM biopsies were obtained before and after the intervention and analyzed by ELISA and RT-PCR. The intervention reduced body weight (P < 0.001) and increased insulin sensitivity (homeostasis model assessment; P < 0.05). Plasma adiponectin (P < 0.001) increased, and C-reactive protein (P < 0.05), IL-6 (P < 0.01), IL-8 (P < 0.05), and monocyte chemoattractant protein-1 (P < 0.01) decreased. AT inflammation was reduced, determined from an increased mRNA expression of adiponectin (P < 0.001) and a decreased expression of macrophage-specific markers (CD14, CD68), IL-6, IL-8, and tumor necrosis factor-alpha (P < 0.01). After adjusting for macrophage infiltration in AT, only IL-6 mRNA was decreased (P < 0.05). Only very low levels of inflammatory markers were found in SM. The intervention had no effect on adiponectin receptor 1 and 2 mRNA in AT or SM. Thus hypocaloric diet and increased physical activity improved insulin sensitivity and reduced low-grade inflammation. Markers of inflammation were particularly reduced in AT, whereas SM does not contribute to this attenuation of whole body inflammation.
AB - Obesity is associated with low-grade inflammation, insulin resistance, type 2 diabetes, and cardiovascular disease. This study investigated the effect of a 15-wk lifestyle intervention (hypocaloric diet and daily exercise) on inflammatory markers in plasma, adipose tissue (AT), and skeletal muscle (SM) in 27 severely obese subjects (mean body mass index: 45.8 kg/m2). Plasma samples, subcutaneous abdominal AT biopsies, and vastus lateralis SM biopsies were obtained before and after the intervention and analyzed by ELISA and RT-PCR. The intervention reduced body weight (P < 0.001) and increased insulin sensitivity (homeostasis model assessment; P < 0.05). Plasma adiponectin (P < 0.001) increased, and C-reactive protein (P < 0.05), IL-6 (P < 0.01), IL-8 (P < 0.05), and monocyte chemoattractant protein-1 (P < 0.01) decreased. AT inflammation was reduced, determined from an increased mRNA expression of adiponectin (P < 0.001) and a decreased expression of macrophage-specific markers (CD14, CD68), IL-6, IL-8, and tumor necrosis factor-alpha (P < 0.01). After adjusting for macrophage infiltration in AT, only IL-6 mRNA was decreased (P < 0.05). Only very low levels of inflammatory markers were found in SM. The intervention had no effect on adiponectin receptor 1 and 2 mRNA in AT or SM. Thus hypocaloric diet and increased physical activity improved insulin sensitivity and reduced low-grade inflammation. Markers of inflammation were particularly reduced in AT, whereas SM does not contribute to this attenuation of whole body inflammation.
U2 - 10.1152/ajpendo.00506.2005
DO - 10.1152/ajpendo.00506.2005
M3 - Journal article
C2 - 16352667
VL - 290
SP - E961-7
JO - American Journal of Physiology - Endocrinology and Metabolism
JF - American Journal of Physiology - Endocrinology and Metabolism
SN - 0193-1849
IS - 5
ER -