Abstract
| Originalsprog | Engelsk |
|---|---|
| Tidsskrift | Clinical and Experimental Immunology |
| Vol/bind | 113 |
| Udgave nummer | 3 |
| Sider (fra-til) | 394-400 |
| Antal sider | 6 |
| ISSN | 0009-9104 |
| Status | Udgivet - 1998 |
Bibliografisk note
Keywords: 3T3 Cells; Animals; Antibodies, Monoclonal; Antigens, CD5; Antigens, Differentiation; Calcium; Galectin 3; Lymphocyte Activation; Membrane Proteins; Mice; Molecular Weight; Receptor-CD3 Complex, Antigen, T-Cell; Receptors, Immunologic; Receptors, Lipoprotein; Receptors, Scavenger; Scavenger Receptors, Class BCitationsformater
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I: Clinical and Experimental Immunology, Bind 113, Nr. 3, 1998, s. 394-400.
Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › peer review
}
TY - JOUR
T1 - Differential effect on TCR:CD3 stimulation of a 90-kD glycoprotein (gp90/Mac-2BP), a member of the scavenger receptor cysteine-rich domain protein family.
AU - Silvestri, B
AU - Calderazzo, F
AU - Coppola, V
AU - Rosato, A
AU - Iacobelli, S
AU - Natoli, C
AU - Ullrich, A
AU - Sures, I
AU - Azam, M
AU - Brakebusch, Cord Herbert
AU - Chieco-Bianchi, L
AU - Amadori, A
N1 - Keywords: 3T3 Cells; Animals; Antibodies, Monoclonal; Antigens, CD5; Antigens, Differentiation; Calcium; Galectin 3; Lymphocyte Activation; Membrane Proteins; Mice; Molecular Weight; Receptor-CD3 Complex, Antigen, T-Cell; Receptors, Immunologic; Receptors, Lipoprotein; Receptors, Scavenger; Scavenger Receptors, Class B
PY - 1998
Y1 - 1998
N2 - We studied the effects of a 90-kD glycoprotein (gp90/Mac-2BP) belonging to the scavenger receptor family, present in normal serum and at increased levels in inflammatory disease and cancer patients, on some T cell function parameters. Whereas the lymphocyte proliferative response to non-specific mitogens such as phytohaemagglutinin (PHA) and concanavalin A (Con A), but not pokeweed mitogen (PWM), was strongly reduced, probably due to the lectin-binding properties of gp90/Mac-2BP, the response to T cell receptor (TCR) agonists such as superantigens and allogeneic cells was potentiated. When lymphocytes were stimulated with different anti-TCR:CD3 MoAbs, both in soluble and solid-phase form, gp90/Mac-2BP was able to down-regulate the proliferative response to anti-CD3 MoAb, whereas the response to anti-TCR alphabeta MoAb was enhanced. A similar differential effect was observed when a MoAb against CD5 (another member of the scavenger receptor superfamily) was added to anti-CD3 or anti-TCR-stimulated cells; anti-CD5 MoAb strongly down-modulated the CD3-mediated response, whereas its presence in culture was associated with potentiation of the response to TCR alphabeta agonists. gp90/Mac-2BP was able per se to up-regulate Ca2+ levels in freshly isolated lymphocytes; moreover, its presence in culture was associated with increased Ca2+ mobilization following stimulation with anti-TCR alphabeta, but not anti-CD3 MoAb. These data indicate that gp90/Mac-2BP could be able to influence some immune responses, possibly through multiple homologous interactions with other members of the scavenger receptor family; moreover, our findings suggest that signalling through the different components of the TCR:CD3 complex may follow distinct activation pathways into the cells.
AB - We studied the effects of a 90-kD glycoprotein (gp90/Mac-2BP) belonging to the scavenger receptor family, present in normal serum and at increased levels in inflammatory disease and cancer patients, on some T cell function parameters. Whereas the lymphocyte proliferative response to non-specific mitogens such as phytohaemagglutinin (PHA) and concanavalin A (Con A), but not pokeweed mitogen (PWM), was strongly reduced, probably due to the lectin-binding properties of gp90/Mac-2BP, the response to T cell receptor (TCR) agonists such as superantigens and allogeneic cells was potentiated. When lymphocytes were stimulated with different anti-TCR:CD3 MoAbs, both in soluble and solid-phase form, gp90/Mac-2BP was able to down-regulate the proliferative response to anti-CD3 MoAb, whereas the response to anti-TCR alphabeta MoAb was enhanced. A similar differential effect was observed when a MoAb against CD5 (another member of the scavenger receptor superfamily) was added to anti-CD3 or anti-TCR-stimulated cells; anti-CD5 MoAb strongly down-modulated the CD3-mediated response, whereas its presence in culture was associated with potentiation of the response to TCR alphabeta agonists. gp90/Mac-2BP was able per se to up-regulate Ca2+ levels in freshly isolated lymphocytes; moreover, its presence in culture was associated with increased Ca2+ mobilization following stimulation with anti-TCR alphabeta, but not anti-CD3 MoAb. These data indicate that gp90/Mac-2BP could be able to influence some immune responses, possibly through multiple homologous interactions with other members of the scavenger receptor family; moreover, our findings suggest that signalling through the different components of the TCR:CD3 complex may follow distinct activation pathways into the cells.
M3 - Journal article
C2 - 9737668
SN - 0009-9104
VL - 113
SP - 394
EP - 400
JO - Clinical and Experimental Immunology
JF - Clinical and Experimental Immunology
IS - 3
ER -