Differential modulation of P-glycoprotein expression by dexamethasone and 3-methycholanthrene in rat hepatocyte primary cultures

Spontaneous and culture condition-dependent changes in P-glycoprotein expression and activity have been monitored in primary cultures of rat hepatocytes by using immuno-blotting, PCR and fluorimetric techniques. In hepatocytes cultured in basal medium without addition of dexamethasone or 3-methylcholanthrene, mdr mRNA and P-glycoprotein increased progressively throughout a 72 h culture period, in concert with an enhancement in the ability to extrude the fluorescent dye Rhodamine-123. Addition of 1μM dexamethasone to the culture medium slowed down the increase in mdr mRNA and P-glycoprotein, while inducing a significant increase in the efficiency of R-123 efflux. Addition of either 100 nM or 10 μM DEX produced different changes in mdr mRNA and protein, unrelated to the rate of Rhodamine-123 extrusion. When 50 μM 3-methylcholanthrene was added to the culture medium in the absence of any hormone supplementation, no significant changes in P-glycoprotein activity and expression took place, in comparison with control cultures. On the contrary, in the presence of dexamethasone (100 nM and 1μM), 3-methylcholanthrene induced an increase in mdr mRNA and in the amount of immunoblottable protein during culture, without producing any concomitant increase in the efficiency to extrude Rhodamine-123. The last phenomenon resulted to be an artefact, since 3-methylcholanthrene was shown to inhibit Rhodamine-123 transport competitively. These results indicate that rat hepatocyte P-glycoprotein may be variously modulated in vitro, by supplementing culture medium with hormones and/or xeno-biotics. Functional activity of the P-glycoprotein is not necessarily related with protein amount and/or mdr RNA.