TY - JOUR
T1 - Dimerization effect of sucrose octasulfate on rat FGF1.
AU - Kulahin, Nikolaj
AU - Kiselyov, Vladislav
AU - Kochoyan, Artur
AU - Kristensen, Ole
AU - Kastrup, Jette Sandholm Jensen
AU - Berezin, Vladimir
AU - Bock, Elisabeth Marianne
AU - Gajhede, Michael
N1 - Paper id:: 18540049
PY - 2008
Y1 - 2008
N2 - Fibroblast growth factors (FGFs) constitute a family of at least 23 structurally related heparin-binding proteins that are involved in regulation of cell growth, survival, differentiation and migration. Sucrose octasulfate (SOS), a chemical analogue of heparin, has been demonstrated to activate FGF signalling pathways. The structure of rat FGF1 crystallized in the presence of SOS has been determined at 2.2 A resolution. SOS-mediated dimerization of FGF1 was observed, which was further supported by gel-filtration experiments. The major contributors to the sulfate-binding sites in rat FGF1 are Lys113, Lys118, Arg122 and Lys128. An arginine at position 116 is a consensus residue in mammalian FGF molecules; however, it is a serine in rat FGF1. This difference may be important for SOS-mediated FGF1 dimerization in rat.
AB - Fibroblast growth factors (FGFs) constitute a family of at least 23 structurally related heparin-binding proteins that are involved in regulation of cell growth, survival, differentiation and migration. Sucrose octasulfate (SOS), a chemical analogue of heparin, has been demonstrated to activate FGF signalling pathways. The structure of rat FGF1 crystallized in the presence of SOS has been determined at 2.2 A resolution. SOS-mediated dimerization of FGF1 was observed, which was further supported by gel-filtration experiments. The major contributors to the sulfate-binding sites in rat FGF1 are Lys113, Lys118, Arg122 and Lys128. An arginine at position 116 is a consensus residue in mammalian FGF molecules; however, it is a serine in rat FGF1. This difference may be important for SOS-mediated FGF1 dimerization in rat.
U2 - 10.1107/S174430910801066X
DO - 10.1107/S174430910801066X
M3 - Journal article
C2 - 18540049
SN - 1744-3091
VL - 64
SP - 448
EP - 452
JO - Acta Crystallographica. Section F : Structural Biology and Crystallization Communications
JF - Acta Crystallographica. Section F : Structural Biology and Crystallization Communications
IS - Pt6
ER -