Direct DNA extraction from Mycobacterium tuberculosis frozen stocks as a reculture-independent approach to whole-genome sequencing

K. Bjorn-Mortensen; J. Zallet; T. Lillebaek; A. B. Andersen; S. Niemann; E. M. Rasmussen; T. A. Kohl

doi:10.1128/JCM.00662-15

Direct DNA extraction from Mycobacterium tuberculosis frozen stocks as a reculture-independent approach to whole-genome sequencing

K. Bjorn-Mortensen^*, J. Zallet, T. Lillebaek, A. B. Andersen, S. Niemann, E. M. Rasmussen, T. A. Kohl

^*Corresponding author af dette arbejde

Institut for Klinisk Medicin

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › peer review

19 Citationer (Scopus)

Abstract

Culturing before DNA extraction represents a major time-consuming step in whole-genome sequencing of slow-growing bacteria, such as Mycobacterium tuberculosis. We report a workflow to extract DNA from frozen isolates without reculturing. Prepared libraries and sequence data were comparable with results from recultured aliquots of the same stocks.

Originalsprog	Engelsk
Tidsskrift	Journal of clinical microbiology
Vol/bind	53
Udgave nummer	8
Sider (fra-til)	2716-2719
Antal sider	4
ISSN	0095-1137
DOI	https://doi.org/10.1128/JCM.00662-15
Status	Udgivet - 1 aug. 2015

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Citationsformater

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AU - Andersen, A. B.

AU - Niemann, S.

AU - Rasmussen, E. M.

AU - Kohl, T. A.

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AB - Culturing before DNA extraction represents a major time-consuming step in whole-genome sequencing of slow-growing bacteria, such as Mycobacterium tuberculosis. We report a workflow to extract DNA from frozen isolates without reculturing. Prepared libraries and sequence data were comparable with results from recultured aliquots of the same stocks.

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DO - 10.1128/JCM.00662-15

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