TY - JOUR
T1 - Direct effect of gonadal and contraceptive steroids on insulin release from mouse pancreatic islets in organ culture
AU - Nielsen, Jens Høiriis
PY - 1984/2
Y1 - 1984/2
N2 - Sex steroids are supposed to contribute to the normal glucose homeostasis and to the altered glucose and insulin metabolism in pregnancy and during contraception. In the present study isolated mouse pancreatic islets were maintained in tissue culture medium RPMI 1640 supplemented with 0.5% newborn calf serum and 100 ng/ml of one of the following steroids: oestradiol, progesterone, testosterone, megestrol acetate, medroxyprogesterone, chlormadinone acetate, norethynodrel, norethindrone acetate, and ethynyloestradiol. Release of insulin to the culture medium was measured during a 2 week culture period, and the islet content of insulin, glucagon, and DNA was measured at the end of the period. It was found that progesterone and its derivatives megestrol acetate, medroxyprogesterone, and chlormadinone caused a 2-fold increase in insulin release during the culture period. When islets cultured in the presence of oestradiol, progesterone, or testosterone were subjected to 30 min stimulation with 5.5, 11, 22 mmol/l glucose, only the progesterone-treated islets released more insulin in response to glucose than the control islets. It is concluded that progesterone and its derivatives have a direct effect on the glucose-stimulated insulin release probably by increasing the glucose sensitivity. The results suggest that the alterations in glucose and insulin metabolism in pregnancy and during treatment with certain oral contraceptives may in part be due to a direct effect of progestins on the beta-cell.
AB - Sex steroids are supposed to contribute to the normal glucose homeostasis and to the altered glucose and insulin metabolism in pregnancy and during contraception. In the present study isolated mouse pancreatic islets were maintained in tissue culture medium RPMI 1640 supplemented with 0.5% newborn calf serum and 100 ng/ml of one of the following steroids: oestradiol, progesterone, testosterone, megestrol acetate, medroxyprogesterone, chlormadinone acetate, norethynodrel, norethindrone acetate, and ethynyloestradiol. Release of insulin to the culture medium was measured during a 2 week culture period, and the islet content of insulin, glucagon, and DNA was measured at the end of the period. It was found that progesterone and its derivatives megestrol acetate, medroxyprogesterone, and chlormadinone caused a 2-fold increase in insulin release during the culture period. When islets cultured in the presence of oestradiol, progesterone, or testosterone were subjected to 30 min stimulation with 5.5, 11, 22 mmol/l glucose, only the progesterone-treated islets released more insulin in response to glucose than the control islets. It is concluded that progesterone and its derivatives have a direct effect on the glucose-stimulated insulin release probably by increasing the glucose sensitivity. The results suggest that the alterations in glucose and insulin metabolism in pregnancy and during treatment with certain oral contraceptives may in part be due to a direct effect of progestins on the beta-cell.
KW - Animals
KW - DNA
KW - Female
KW - Glucagon
KW - Gonadal Steroid Hormones
KW - Insulin
KW - Islets of Langerhans
KW - Male
KW - Mice
KW - Mice, Inbred Strains
KW - Organ Culture Techniques
M3 - Journal article
C2 - 6364674
VL - 105
SP - 245
EP - 250
JO - European Journal of Endocrinology
JF - European Journal of Endocrinology
SN - 0804-4643
IS - 2
ER -