Abstract
| Originalsprog | Engelsk |
|---|---|
| Tidsskrift | American Journal of Physiology: Endocrinology and Metabolism |
| Vol/bind | 285 |
| Udgave nummer | 4 |
| Sider (fra-til) | E836-44 |
| ISSN | 0193-1849 |
| DOI | |
| Status | Udgivet - 2003 |
Bibliografisk note
Keywords: Animals; Colchicine; Deoxyglucose; Forelimb; Glucose Transporter Type 4; Hindlimb; Insulin; Male; Microtubules; Monosaccharide Transport Proteins; Muscle Contraction; Muscle Fibers; Muscle Proteins; Rats; Stress, MechanicalAdgang til dokumentet
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Disruption of microtubules in rat skeletal muscle does not inhibit insulin- or contraction-stimulated glucose transport. / Ai, Hua; Ralston, Evelyn; Lauritzen, Hans P M M; Galbo, Henrik; Ploug, Thorkil.
I: American Journal of Physiology: Endocrinology and Metabolism, Bind 285, Nr. 4, 2003, s. E836-44.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › peer review
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TY - JOUR
T1 - Disruption of microtubules in rat skeletal muscle does not inhibit insulin- or contraction-stimulated glucose transport.
AU - Ai, Hua
AU - Ralston, Evelyn
AU - Lauritzen, Hans P M M
AU - Galbo, Henrik
AU - Ploug, Thorkil
N1 - Keywords: Animals; Colchicine; Deoxyglucose; Forelimb; Glucose Transporter Type 4; Hindlimb; Insulin; Male; Microtubules; Monosaccharide Transport Proteins; Muscle Contraction; Muscle Fibers; Muscle Proteins; Rats; Stress, Mechanical
PY - 2003
Y1 - 2003
N2 - Insulin and muscle contractions stimulate glucose transport in skeletal muscle through a translocation of intracellular GLUT4 glucose transporters to the cell surface. Judged by immunofluorescence microscopy, part of the GLUT4 storage sites is associated with the extensive microtubule cytoskeleton found in all muscle fibers. Here, we test whether microtubules are required mediators of the effect of insulin and contractions. In three different incubated rat muscles with distinct fiber type composition, depolymerization of microtubules with colchicine for < or =8 h did not inhibit insulin- or contraction-stimulated 2-deoxyglucose transport or force production. On the contrary, colchicine at least partially prevented the approximately 30% decrease in insulin-stimulated transport that specifically developed during 8 h of incubation in soleus muscle but not in flexor digitorum brevis or epitrochlearis muscles. In contrast, nocodazole, another microtubule-disrupting drug, rapidly and dose dependently blocked insulin- and contraction-stimulated glucose transport. A similar discrepancy between colchicine and nocodazole was also found in their ability to block glucose transport in muscle giant "ghost" vesicles. This suggests that the ability of insulin and contractions to stimulate glucose transport in muscle does not require an intact microtubule network and that nocodazole inhibits glucose transport independently of its microtubule-disrupting effect.
AB - Insulin and muscle contractions stimulate glucose transport in skeletal muscle through a translocation of intracellular GLUT4 glucose transporters to the cell surface. Judged by immunofluorescence microscopy, part of the GLUT4 storage sites is associated with the extensive microtubule cytoskeleton found in all muscle fibers. Here, we test whether microtubules are required mediators of the effect of insulin and contractions. In three different incubated rat muscles with distinct fiber type composition, depolymerization of microtubules with colchicine for < or =8 h did not inhibit insulin- or contraction-stimulated 2-deoxyglucose transport or force production. On the contrary, colchicine at least partially prevented the approximately 30% decrease in insulin-stimulated transport that specifically developed during 8 h of incubation in soleus muscle but not in flexor digitorum brevis or epitrochlearis muscles. In contrast, nocodazole, another microtubule-disrupting drug, rapidly and dose dependently blocked insulin- and contraction-stimulated glucose transport. A similar discrepancy between colchicine and nocodazole was also found in their ability to block glucose transport in muscle giant "ghost" vesicles. This suggests that the ability of insulin and contractions to stimulate glucose transport in muscle does not require an intact microtubule network and that nocodazole inhibits glucose transport independently of its microtubule-disrupting effect.
U2 - 10.1152/ajpendo.00238.2002
DO - 10.1152/ajpendo.00238.2002
M3 - Journal article
C2 - 12746214
VL - 285
SP - E836-44
JO - American Journal of Physiology - Endocrinology and Metabolism
JF - American Journal of Physiology - Endocrinology and Metabolism
SN - 0193-1849
IS - 4
ER -