Abstract
| Originalsprog | Engelsk |
|---|---|
| Tidsskrift | Journal of Cellular Physiology |
| Vol/bind | 218 |
| Udgave nummer | 2 |
| Sider (fra-til) | 444-9 |
| Antal sider | 5 |
| ISSN | 0021-9541 |
| DOI | |
| Status | Udgivet - 2009 |
Bibliografisk note
(c) 2008 Wiley-Liss, Inc.Adgang til dokumentet
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Distinct expression of muscle-specific microRNAs (myomirs) in brown adipocytes. / Walden, Tomas B; Timmons, James A; Keller, Pernille; Nedergaard, Jan; Cannon, Barbara.
I: Journal of Cellular Physiology, Bind 218, Nr. 2, 2009, s. 444-9.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › peer review
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TY - JOUR
T1 - Distinct expression of muscle-specific microRNAs (myomirs) in brown adipocytes
AU - Walden, Tomas B
AU - Timmons, James A
AU - Keller, Pernille
AU - Nedergaard, Jan
AU - Cannon, Barbara
N1 - Keywords: Adipocytes, Brown; Adipogenesis; Animals; Biological Markers; Cell Line; Cell Lineage; Gene Expression Regulation; Male; Mice; MicroRNAs; Muscle Development; Muscle, Skeletal; Organ Specificity; RNA, Messenger
PY - 2009
Y1 - 2009
N2 - MicroRNAs, a novel class of post-transcriptional gene regulators, have been demonstrated to be involved in several cellular processes regulating the expression of protein-coding genes. Here we examine murine white and brown primary cell cultures for differential expression of miRNAs. The adipogenesis-related miRNA miR-143 was highly expressed in mature white adipocytes but was low in mature brown adipocytes. Three classical "myogenic" miRNAs miR-1, miR-133a and miR-206 were absent from white adipocytes but were specifically expressed both in brown pre- and mature adipocytes, reinforcing the concept that brown adipocytes and myocytes derive from a common cell lineage that specifies energy-dissipating cells. Augmentation of adipocyte differentiation status with norepinephrine or rosiglitazone did not affect the expression of the above miRNAs, the expression levels of which were thus innately regulated. However, expression of the miRNA miR-455 was enhanced during brown adipocyte differentiation, similarly to the expression pattern of the brown adipocyte differentiation marker UCP1. In conclusion, miRNAs are differentially expressed in white and brown adipocytes and may be important in defining the common precursor cell for myocytes and brown adipocytes and thus have distinct roles in energy-storing versus energy-dissipating cells.
AB - MicroRNAs, a novel class of post-transcriptional gene regulators, have been demonstrated to be involved in several cellular processes regulating the expression of protein-coding genes. Here we examine murine white and brown primary cell cultures for differential expression of miRNAs. The adipogenesis-related miRNA miR-143 was highly expressed in mature white adipocytes but was low in mature brown adipocytes. Three classical "myogenic" miRNAs miR-1, miR-133a and miR-206 were absent from white adipocytes but were specifically expressed both in brown pre- and mature adipocytes, reinforcing the concept that brown adipocytes and myocytes derive from a common cell lineage that specifies energy-dissipating cells. Augmentation of adipocyte differentiation status with norepinephrine or rosiglitazone did not affect the expression of the above miRNAs, the expression levels of which were thus innately regulated. However, expression of the miRNA miR-455 was enhanced during brown adipocyte differentiation, similarly to the expression pattern of the brown adipocyte differentiation marker UCP1. In conclusion, miRNAs are differentially expressed in white and brown adipocytes and may be important in defining the common precursor cell for myocytes and brown adipocytes and thus have distinct roles in energy-storing versus energy-dissipating cells.
U2 - 10.1002/jcp.21621
DO - 10.1002/jcp.21621
M3 - Journal article
C2 - 18937285
VL - 218
SP - 444
EP - 449
JO - Journal of Cellular Physiology
JF - Journal of Cellular Physiology
SN - 0021-9541
IS - 2
ER -