Distinct triterpene synthases in the laticifers of Euphorbia lathyris

Edith Forestier; Carmen Romero-Segura; Irini Pateraki; Emilio Centeno; Vincent Compagnon; Myriam Preiss; Anne Berna; Albert Boronat; Thomas J. Bach; Sylvain Darnet; Hubert Schaller

doi:10.1038/s41598-019-40905-y

Distinct triterpene synthases in the laticifers of Euphorbia lathyris

Edith Forestier
, Carmen Romero-Segura
, Irini Pateraki
, Emilio Centeno
, Vincent Compagnon
, Myriam Preiss
, Anne Berna
, Albert Boronat
, Thomas J. Bach
, Sylvain Darnet
, Hubert Schaller^*

^*Corresponding author af dette arbejde

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › peer review

Abstract

Euphorbia lathyris was proposed about fifty years ago as a potential agroenergetic crop. The tremendous amounts of triterpenes present in its latex has driven investigations for transforming this particular biological fluid into an industrial hydrocarbon source. The huge accumulation of terpenes in the latex of many plant species represent a challenging question regarding cellular homeostasis. In fact, the enzymes, the mechanisms and the controllers that tune the amount of products accumulated in specialized compartments (to fulfill ecological roles) or deposited at important sites (as essential factors) are not known. Here, we have isolated oxidosqualene cyclases highly expressed in the latex of Euphorbia lathyris. This triterpene biosynthetic machinery is made of distinct paralogous enzymes responsible for the massive accumulation of steroidal and non-steroidal tetracyclic triterpenes. More than eighty years after the isolation of butyrospermol from shea butter (Heilbronn IM, Moffet GL, and Spring FS J. Chem. Soc. 1934, 1583), a butyrospermol synthase is characterized in this work using yeast and in folia heterologous expression assays.

Originalsprog	Engelsk
Artikelnummer	4840
Tidsskrift	Scientific Reports
Vol/bind	9
Udgave nummer	1
ISSN	2045-2322
DOI	https://doi.org/10.1038/s41598-019-40905-y
Status	Udgivet - 2019
Udgivet eksternt	Ja

Bibliografisk note

Publisher Copyright:
© 2019, The Author(s).

Finansiering

The authors are grateful to Profs. Yukuta Ebizuka and Tetsuo Kushiro for a gift of the GIL77 (erg7) yeast strain, to Dr. Pascaline Ullmann for valuable advice on yeast transformation, to Dr. Raphaël Lugan and Dr. Dimitri Heintz for GC-MS implementation, and to Dr. Bruno Vincent for NMR analysis. This work was funded by a Plant Knowledge Based Bio Economy program ‘EULAFUEL’ (Plant KBBE 2009–2014) to Prof. Thomas J. Bach and Dr. Hubert Schaller (ANR09-KBBE-003-002), and to Prof. Albert Boronat (PLE2009-0003 from Ministerio de Ciencia e Innovación, Spain). The cooperation between Universidade Federal do Pará, Belém, Brazil, and the Institut de Biologie Moléculaire des Plantes is framed by a French-Brazilian International Associated Laboratory LIA N°1170 (Palmheat, Drs Sylvain Darnet and Hubert Schaller).

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Citationsformater

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title = "Distinct triterpene synthases in the laticifers of Euphorbia lathyris",

abstract = "Euphorbia lathyris was proposed about fifty years ago as a potential agroenergetic crop. The tremendous amounts of triterpenes present in its latex has driven investigations for transforming this particular biological fluid into an industrial hydrocarbon source. The huge accumulation of terpenes in the latex of many plant species represent a challenging question regarding cellular homeostasis. In fact, the enzymes, the mechanisms and the controllers that tune the amount of products accumulated in specialized compartments (to fulfill ecological roles) or deposited at important sites (as essential factors) are not known. Here, we have isolated oxidosqualene cyclases highly expressed in the latex of Euphorbia lathyris. This triterpene biosynthetic machinery is made of distinct paralogous enzymes responsible for the massive accumulation of steroidal and non-steroidal tetracyclic triterpenes. More than eighty years after the isolation of butyrospermol from shea butter (Heilbronn IM, Moffet GL, and Spring FS J. Chem. Soc. 1934, 1583), a butyrospermol synthase is characterized in this work using yeast and in folia heterologous expression assays.",

author = "Edith Forestier and Carmen Romero-Segura and Irini Pateraki and Emilio Centeno and Vincent Compagnon and Myriam Preiss and Anne Berna and Albert Boronat and Bach, \{Thomas J.\} and Sylvain Darnet and Hubert Schaller",

note = "Funding Information: The authors are grateful to Profs. Yukuta Ebizuka and Tetsuo Kushiro for a gift of the GIL77 (erg7) yeast strain, to Dr. Pascaline Ullmann for valuable advice on yeast transformation, to Dr. Rapha{\"e}l Lugan and Dr. Dimitri Heintz for GC-MS implementation, and to Dr. Bruno Vincent for NMR analysis. This work was funded by a Plant Knowledge Based Bio Economy program {\textquoteleft}EULAFUEL{\textquoteright} (Plant KBBE 2009–2014) to Prof. Thomas J. Bach and Dr. Hubert Schaller (ANR09-KBBE-003-002), and to Prof. Albert Boronat (PLE2009-0003 from Ministerio de Ciencia e Innovaci{\'o}n, Spain). The cooperation between Universidade Federal do Par{\'a}, Bel{\'e}m, Brazil, and the Institut de Biologie Mol{\'e}culaire des Plantes is framed by a French-Brazilian International Associated Laboratory LIA N°1170 (Palmheat, Drs Sylvain Darnet and Hubert Schaller). Publisher Copyright: {\textcopyright} 2019, The Author(s).",

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doi = "10.1038/s41598-019-40905-y",

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T1 - Distinct triterpene synthases in the laticifers of Euphorbia lathyris

AU - Forestier, Edith

AU - Romero-Segura, Carmen

AU - Pateraki, Irini

AU - Centeno, Emilio

AU - Compagnon, Vincent

AU - Preiss, Myriam

AU - Berna, Anne

AU - Boronat, Albert

AU - Bach, Thomas J.

AU - Darnet, Sylvain

AU - Schaller, Hubert

N1 - Funding Information: The authors are grateful to Profs. Yukuta Ebizuka and Tetsuo Kushiro for a gift of the GIL77 (erg7) yeast strain, to Dr. Pascaline Ullmann for valuable advice on yeast transformation, to Dr. Raphaël Lugan and Dr. Dimitri Heintz for GC-MS implementation, and to Dr. Bruno Vincent for NMR analysis. This work was funded by a Plant Knowledge Based Bio Economy program ‘EULAFUEL’ (Plant KBBE 2009–2014) to Prof. Thomas J. Bach and Dr. Hubert Schaller (ANR09-KBBE-003-002), and to Prof. Albert Boronat (PLE2009-0003 from Ministerio de Ciencia e Innovación, Spain). The cooperation between Universidade Federal do Pará, Belém, Brazil, and the Institut de Biologie Moléculaire des Plantes is framed by a French-Brazilian International Associated Laboratory LIA N°1170 (Palmheat, Drs Sylvain Darnet and Hubert Schaller). Publisher Copyright: © 2019, The Author(s).

PY - 2019

Y1 - 2019

N2 - Euphorbia lathyris was proposed about fifty years ago as a potential agroenergetic crop. The tremendous amounts of triterpenes present in its latex has driven investigations for transforming this particular biological fluid into an industrial hydrocarbon source. The huge accumulation of terpenes in the latex of many plant species represent a challenging question regarding cellular homeostasis. In fact, the enzymes, the mechanisms and the controllers that tune the amount of products accumulated in specialized compartments (to fulfill ecological roles) or deposited at important sites (as essential factors) are not known. Here, we have isolated oxidosqualene cyclases highly expressed in the latex of Euphorbia lathyris. This triterpene biosynthetic machinery is made of distinct paralogous enzymes responsible for the massive accumulation of steroidal and non-steroidal tetracyclic triterpenes. More than eighty years after the isolation of butyrospermol from shea butter (Heilbronn IM, Moffet GL, and Spring FS J. Chem. Soc. 1934, 1583), a butyrospermol synthase is characterized in this work using yeast and in folia heterologous expression assays.

AB - Euphorbia lathyris was proposed about fifty years ago as a potential agroenergetic crop. The tremendous amounts of triterpenes present in its latex has driven investigations for transforming this particular biological fluid into an industrial hydrocarbon source. The huge accumulation of terpenes in the latex of many plant species represent a challenging question regarding cellular homeostasis. In fact, the enzymes, the mechanisms and the controllers that tune the amount of products accumulated in specialized compartments (to fulfill ecological roles) or deposited at important sites (as essential factors) are not known. Here, we have isolated oxidosqualene cyclases highly expressed in the latex of Euphorbia lathyris. This triterpene biosynthetic machinery is made of distinct paralogous enzymes responsible for the massive accumulation of steroidal and non-steroidal tetracyclic triterpenes. More than eighty years after the isolation of butyrospermol from shea butter (Heilbronn IM, Moffet GL, and Spring FS J. Chem. Soc. 1934, 1583), a butyrospermol synthase is characterized in this work using yeast and in folia heterologous expression assays.

U2 - 10.1038/s41598-019-40905-y

DO - 10.1038/s41598-019-40905-y

M3 - Journal article

C2 - 30886213

AN - SCOPUS:85063035603

SN - 2045-2322

VL - 9

JO - Scientific Reports

JF - Scientific Reports

IS - 1

M1 - 4840

ER -