Diversification of the CRISPR Toolbox: Applications of CRISPR-Cas Systems Beyond Genome Editing

Sarah Balderston; Gabrielle Clouse; Juan José Ripoll; Grace K. Pratt; Giedrius Gasiunas; Jens Ole Bock; Eric Paul Bennett; Kiana Aran

doi:10.1089/crispr.2020.0137

Diversification of the CRISPR Toolbox: Applications of CRISPR-Cas Systems Beyond Genome Editing

Sarah Balderston, Gabrielle Clouse, Juan José Ripoll, Grace K. Pratt, Giedrius Gasiunas, Jens Ole Bock, Eric Paul Bennett, Kiana Aran^*

^*Corresponding author af dette arbejde

Sektion 03

Publikation: Bidrag til tidsskrift › Review › Forskning › peer review

8 Citationer (Scopus)

Abstract

The discovery of CRISPR has revolutionized the field of genome engineering, but the potential of this technology is far from reaching its limits. In this review, we explore the broad range of applications of CRISPR technology to highlight the rapid expansion of the field beyond gene editing alone. It has been demonstrated that CRISPR technology can control gene expression, spatiotemporally image the genome in vivo, and detect specific nucleic acid sequences for diagnostics. In addition, new technologies are under development to improve CRISPR quality controls for gene editing, thereby improving the reliability of these technologies for therapeutics and beyond. These are just some of the many CRISPR tools that have been developed in recent years, and the toolbox continues to diversify.

Originalsprog	Engelsk
Tidsskrift	CRISPR Journal
Vol/bind	4
Udgave nummer	3
Sider (fra-til)	400-415
Antal sider	16
ISSN	2573-1599
DOI	https://doi.org/10.1089/crispr.2020.0137
Status	Udgivet - 2021

Bibliografisk note

Funding Information:
This work was funded through NSF #GR750012, NIH #GR750002 and Cardea sponsored research #GR720020.

Publisher Copyright:
© Copyright 2021, Mary Ann Liebert, Inc., publishers 2021.

Adgang til dokumentet

10.1089/crispr.2020.0137

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8418451/pdf/crispr.2020.0137.pdfLicens: Andet

Citationsformater

Diversification of the CRISPR Toolbox : Applications of CRISPR-Cas Systems Beyond Genome Editing. / Balderston, Sarah; Clouse, Gabrielle; Ripoll, Juan José; Pratt, Grace K.; Gasiunas, Giedrius; Bock, Jens Ole; Bennett, Eric Paul; Aran, Kiana.

I: CRISPR Journal, Bind 4, Nr. 3, 2021, s. 400-415.

Publikation: Bidrag til tidsskrift › Review › Forskning › peer review

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