DNA-AgNC Loaded Liposomes for Measuring Cerebral Blood Flow Using Two-Photon Fluorescence Correlation Spectroscopy

Xiaojin Wang; Mikkel B. Liisberg; Georgia L. Nolt; Xu Fu; Cecilia Cerretani; Lan Li; Lance A. Johnson; Tom Vosch; Christopher I. Richards

doi:10.1021/acsnano.3c04489

DNA-AgNC Loaded Liposomes for Measuring Cerebral Blood Flow Using Two-Photon Fluorescence Correlation Spectroscopy

Xiaojin Wang, Mikkel B. Liisberg, Georgia L. Nolt, Xu Fu, Cecilia Cerretani, Lan Li, Lance A. Johnson, Tom Vosch, Christopher I. Richards

Kemisk Institut

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › peer review

10 Citationer (Scopus)

3 Downloads (Pure)

Abstract

Unraveling the transport of drugs and nanocarriers in cerebrovascular networks is important for pharmacokinetic and hemodynamic studies but is challenging due to the complexity of sensing individual particles within the circulatory system of a live animal. Here, we demonstrate that a DNA-stabilized silver nanocluster (DNA-Ag16NC) that emits in the first near-infrared window upon two-photon excitation in the second NIR window can be used for multiphoton in vivo fluorescence correlation spectroscopy for the measurement of cerebral blood flow rates in live mice with high spatial and temporal resolution. To ensure bright and stable emission during in vivo experiments, we loaded DNA-Ag16NCs into liposomes, which served the dual purposes of concentrating the fluorescent label and protecting it from degradation. DNA-Ag16NC-loaded liposomes enabled the quantification of cerebral blood flow velocities within individual vessels of a living mouse.

Originalsprog	Engelsk
Tidsskrift	ACS Nano
Vol/bind	17
Udgave nummer	13
Sider (fra-til)	12862–12874
Antal sider	13
ISSN	1936-0851
DOI	https://doi.org/10.1021/acsnano.3c04489
Status	Udgivet - 2023

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10.1021/acsnano.3c04489

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DNA-AgNC Loaded Liposomes for Measuring Cerebral Blood Flow Using Two-Photon Fluorescence Correlation Spectroscopy. / Wang, Xiaojin; Liisberg, Mikkel B.; Nolt, Georgia L.; Fu, Xu; Cerretani, Cecilia; Li, Lan; Johnson, Lance A.; Vosch, Tom; Richards, Christopher I.

I: ACS Nano, Bind 17, Nr. 13, 2023, s. 12862–12874.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › peer review

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abstract = "Unraveling the transport of drugs and nanocarriers in cerebrovascular networks is important for pharmacokinetic and hemodynamic studies but is challenging due to the complexity of sensing individual particles within the circulatory system of a live animal. Here, we demonstrate that a DNA-stabilized silver nanocluster (DNA-Ag16NC) that emits in the first near-infrared window upon two-photon excitation in the second NIR window can be used for multiphoton in vivo fluorescence correlation spectroscopy for the measurement of cerebral blood flow rates in live mice with high spatial and temporal resolution. To ensure bright and stable emission during in vivo experiments, we loaded DNA-Ag16NCs into liposomes, which served the dual purposes of concentrating the fluorescent label and protecting it from degradation. DNA-Ag16NC-loaded liposomes enabled the quantification of cerebral blood flow velocities within individual vessels of a living mouse.",

author = "Xiaojin Wang and Liisberg, {Mikkel B.} and Nolt, {Georgia L.} and Xu Fu and Cecilia Cerretani and Lan Li and Johnson, {Lance A.} and Tom Vosch and Richards, {Christopher I.}",

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T1 - DNA-AgNC Loaded Liposomes for Measuring Cerebral Blood Flow Using Two-Photon Fluorescence Correlation Spectroscopy

AU - Wang, Xiaojin

AU - Liisberg, Mikkel B.

AU - Nolt, Georgia L.

AU - Fu, Xu

AU - Cerretani, Cecilia

AU - Li, Lan

AU - Johnson, Lance A.

AU - Vosch, Tom

AU - Richards, Christopher I.

PY - 2023

Y1 - 2023

N2 - Unraveling the transport of drugs and nanocarriers in cerebrovascular networks is important for pharmacokinetic and hemodynamic studies but is challenging due to the complexity of sensing individual particles within the circulatory system of a live animal. Here, we demonstrate that a DNA-stabilized silver nanocluster (DNA-Ag16NC) that emits in the first near-infrared window upon two-photon excitation in the second NIR window can be used for multiphoton in vivo fluorescence correlation spectroscopy for the measurement of cerebral blood flow rates in live mice with high spatial and temporal resolution. To ensure bright and stable emission during in vivo experiments, we loaded DNA-Ag16NCs into liposomes, which served the dual purposes of concentrating the fluorescent label and protecting it from degradation. DNA-Ag16NC-loaded liposomes enabled the quantification of cerebral blood flow velocities within individual vessels of a living mouse.

AB - Unraveling the transport of drugs and nanocarriers in cerebrovascular networks is important for pharmacokinetic and hemodynamic studies but is challenging due to the complexity of sensing individual particles within the circulatory system of a live animal. Here, we demonstrate that a DNA-stabilized silver nanocluster (DNA-Ag16NC) that emits in the first near-infrared window upon two-photon excitation in the second NIR window can be used for multiphoton in vivo fluorescence correlation spectroscopy for the measurement of cerebral blood flow rates in live mice with high spatial and temporal resolution. To ensure bright and stable emission during in vivo experiments, we loaded DNA-Ag16NCs into liposomes, which served the dual purposes of concentrating the fluorescent label and protecting it from degradation. DNA-Ag16NC-loaded liposomes enabled the quantification of cerebral blood flow velocities within individual vessels of a living mouse.

U2 - 10.1021/acsnano.3c04489

DO - 10.1021/acsnano.3c04489

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