DNA methylation is associated with prenatal exposure to sulfur dioxide and childhood attention-deficit hyperactivity disorder symptoms

Yoon-Jung Choi, Jinwoo Cho, Yun-Chul Hong, Dong-Wook Lee, Sungji Moon, Soo Jin Park, Kyung-Shin Lee, Choong Ho Shin, Young Ah Lee, Bung-Nyun Kim, Zachary Kaminsky, Johanna Inhyang Kim, Youn-Hee Lim

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7 Citationer (Scopus)
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Abstract

Epigenetic influence plays a role in the association between exposure to air pollution and attention deficit hyperactivity disorder (ADHD); however, research regarding sulfur dioxide (SO 2) is scarce. Herein, we investigate the associations between prenatal SO 2 exposure and ADHD rating scale (ARS) at ages 4, 6 and 8 years repeatedly in a mother-child cohort (n = 329). Whole blood samples were obtained at ages 2 and 6 years, and genome-wide DNA methylation (DNAm) was analyzed for 51 children using the Illumina Infinium HumanMethylation BeadChip. We analyzed the associations between prenatal SO 2 exposure and DNAm levels at ages 2 and 6, and further investigated the association between the DNAm and ARS at ages 4, 6 and 8. Prenatal SO 2 exposure was associated with ADHD symptoms. From candidate gene analysis, DNAm levels at the 6 CpGs at age 2 were associated with prenatal SO 2 exposure levels. Of the 6 CpGs, cg07583420 (INS-IGF2) was persistently linked with ARS at ages 4, 6 and 8. Epigenome-wide analysis showed that DNAm at 6733 CpG sites were associated with prenatal SO 2 exposure, of which 58 CpGs involved in Notch signalling pathway were further associated with ARS at age 4, 6 and 8 years, persistently. DNAm at age 6 was not associated with prenatal SO 2 exposure. Changes in DNAm levels associated with prenatal SO 2 exposure during early childhood are associated with increases in ARS in later childhood.

OriginalsprogEngelsk
Artikelnummer3501
TidsskriftScientific Reports
Vol/bind13
Udgave nummer1
Antal sider13
ISSN2045-2322
DOI
StatusUdgivet - 2023

Bibliografisk note

© 2023. The Author(s).

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