Dystroglycan is required for polarizing the epithelial cells and the oocyte in Drosophila

Wu-Min Deng; Martina Schneider; Richard Frock; Casimiro Castillejo-Lopez; Emily Anne Gaman; Stefan Baumgartner; Hannele Ruohola-Baker

Dystroglycan is required for polarizing the epithelial cells and the oocyte in Drosophila

Wu-Min Deng, Martina Schneider, Richard Frock, Casimiro Castillejo-Lopez, Emily Anne Gaman, Stefan Baumgartner, Hannele Ruohola-Baker

Cell- and Neurobiology

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › peer review

158 Citationer (Scopus)

Abstract

Udgivelsesdato: 2003-Jan

Originalsprog	Engelsk
Tidsskrift	Development
Vol/bind	130
Udgave nummer	1
Sider (fra-til)	173-84
Antal sider	11
ISSN	0950-1991
Status	Udgivet - 2003

Bibliografisk note

Keywords: Actins; Amino Acid Sequence; Animals; Animals, Genetically Modified; Cell Polarity; Cytoskeletal Proteins; Cytoskeleton; Drosophila Proteins; Drosophila melanogaster; Dystroglycans; Epithelial Cells; Female; Laminin; Male; Membrane Glycoproteins; Molecular Sequence Data; Mutation; Oocytes; Ovarian Follicle; Sequence Homology, Amino Acid

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http://dev.biologists.org/cgi/content/full/130/1/173

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Citationsformater

@article{06ac9390ccf511dd9473000ea68e967b,

title = "Dystroglycan is required for polarizing the epithelial cells and the oocyte in Drosophila",

abstract = "The transmembrane protein Dystroglycan is a central element of the dystrophin-associated glycoprotein complex, which is involved in the pathogenesis of many forms of muscular dystrophy. Dystroglycan is a receptor for multiple extracellular matrix (ECM) molecules such as Laminin, agrin and perlecan, and plays a role in linking the ECM to the actin cytoskeleton; however, how these interactions are regulated and their basic cellular functions are poorly understood. Using mosaic analysis and RNAi in the model organism Drosophila melanogaster, we show that Dystroglycan is required cell-autonomously for cellular polarity in two different cell types, the epithelial cells (apicobasal polarity) and the oocyte (anteroposterior polarity). Loss of Dystroglycan function in follicle and disc epithelia results in expansion of apical markers to the basal side of cells and overexpression results in a reduced apical localization of these same markers. In Dystroglycan germline clones early oocyte polarity markers fail to be localized to the posterior, and oocyte cortical F-actin organization is abnormal. Dystroglycan is also required non-cell-autonomously to organize the planar polarity of basal actin in follicle cells, possibly by organizing the Laminin ECM. These data suggest that the primary function of Dystroglycan in oogenesis is to organize cellular polarity; and this study sets the stage for analyzing the Dystroglycan complex by using the power of Drosophila molecular genetics.",

author = "Wu-Min Deng and Martina Schneider and Richard Frock and Casimiro Castillejo-Lopez and Gaman, {Emily Anne} and Stefan Baumgartner and Hannele Ruohola-Baker",

note = "Keywords: Actins; Amino Acid Sequence; Animals; Animals, Genetically Modified; Cell Polarity; Cytoskeletal Proteins; Cytoskeleton; Drosophila Proteins; Drosophila melanogaster; Dystroglycans; Epithelial Cells; Female; Laminin; Male; Membrane Glycoproteins; Molecular Sequence Data; Mutation; Oocytes; Ovarian Follicle; Sequence Homology, Amino Acid",

year = "2003",

language = "English",

volume = "130",

pages = "173--84",

journal = "Development",

issn = "0950-1991",

publisher = "The Company of Biologists",

number = "1",

}

TY - JOUR

T1 - Dystroglycan is required for polarizing the epithelial cells and the oocyte in Drosophila

AU - Deng, Wu-Min

AU - Schneider, Martina

AU - Frock, Richard

AU - Castillejo-Lopez, Casimiro

AU - Gaman, Emily Anne

AU - Baumgartner, Stefan

AU - Ruohola-Baker, Hannele

N1 - Keywords: Actins; Amino Acid Sequence; Animals; Animals, Genetically Modified; Cell Polarity; Cytoskeletal Proteins; Cytoskeleton; Drosophila Proteins; Drosophila melanogaster; Dystroglycans; Epithelial Cells; Female; Laminin; Male; Membrane Glycoproteins; Molecular Sequence Data; Mutation; Oocytes; Ovarian Follicle; Sequence Homology, Amino Acid

PY - 2003

Y1 - 2003

N2 - The transmembrane protein Dystroglycan is a central element of the dystrophin-associated glycoprotein complex, which is involved in the pathogenesis of many forms of muscular dystrophy. Dystroglycan is a receptor for multiple extracellular matrix (ECM) molecules such as Laminin, agrin and perlecan, and plays a role in linking the ECM to the actin cytoskeleton; however, how these interactions are regulated and their basic cellular functions are poorly understood. Using mosaic analysis and RNAi in the model organism Drosophila melanogaster, we show that Dystroglycan is required cell-autonomously for cellular polarity in two different cell types, the epithelial cells (apicobasal polarity) and the oocyte (anteroposterior polarity). Loss of Dystroglycan function in follicle and disc epithelia results in expansion of apical markers to the basal side of cells and overexpression results in a reduced apical localization of these same markers. In Dystroglycan germline clones early oocyte polarity markers fail to be localized to the posterior, and oocyte cortical F-actin organization is abnormal. Dystroglycan is also required non-cell-autonomously to organize the planar polarity of basal actin in follicle cells, possibly by organizing the Laminin ECM. These data suggest that the primary function of Dystroglycan in oogenesis is to organize cellular polarity; and this study sets the stage for analyzing the Dystroglycan complex by using the power of Drosophila molecular genetics.

AB - The transmembrane protein Dystroglycan is a central element of the dystrophin-associated glycoprotein complex, which is involved in the pathogenesis of many forms of muscular dystrophy. Dystroglycan is a receptor for multiple extracellular matrix (ECM) molecules such as Laminin, agrin and perlecan, and plays a role in linking the ECM to the actin cytoskeleton; however, how these interactions are regulated and their basic cellular functions are poorly understood. Using mosaic analysis and RNAi in the model organism Drosophila melanogaster, we show that Dystroglycan is required cell-autonomously for cellular polarity in two different cell types, the epithelial cells (apicobasal polarity) and the oocyte (anteroposterior polarity). Loss of Dystroglycan function in follicle and disc epithelia results in expansion of apical markers to the basal side of cells and overexpression results in a reduced apical localization of these same markers. In Dystroglycan germline clones early oocyte polarity markers fail to be localized to the posterior, and oocyte cortical F-actin organization is abnormal. Dystroglycan is also required non-cell-autonomously to organize the planar polarity of basal actin in follicle cells, possibly by organizing the Laminin ECM. These data suggest that the primary function of Dystroglycan in oogenesis is to organize cellular polarity; and this study sets the stage for analyzing the Dystroglycan complex by using the power of Drosophila molecular genetics.

M3 - Journal article

C2 - 12441301

SN - 0950-1991

VL - 130

SP - 173

EP - 184

JO - Development

JF - Development

IS - 1

ER -