Effect of renal nerve activity on tubular sodium and water reabsorption in dog kidneys as determined by the lithium clearance method.

U Abildgaard; N H Holstein-Rathlou; P P Leyssac

Effect of renal nerve activity on tubular sodium and water reabsorption in dog kidneys as determined by the lithium clearance method.

U Abildgaard, N H Holstein-Rathlou, P P Leyssac

Biomedicinsk Institut

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › peer review

24 Citationer (Scopus)

Abstract

Udgivelsesdato: 1986-Feb

Originalsprog	Engelsk
Tidsskrift	Acta Physiologica (Print Edition)
Vol/bind	126
Udgave nummer	2
Sider (fra-til)	251-7
Antal sider	6
ISSN	1748-1708
Status	Udgivet - 1986

Bibliografisk note

Keywords: Amiloride; Animals; Body Water; Denervation; Dogs; Electric Stimulation; Glomerular Filtration Rate; Hemodynamics; Kidney; Kidney Tubules; Lithium; Renal Circulation; Sodium

Citationsformater

@article{789a0250abf111ddb5e9000ea68e967b,

title = "Effect of renal nerve activity on tubular sodium and water reabsorption in dog kidneys as determined by the lithium clearance method.",

abstract = "The reliability of the lithium clearance method in studies of the effect of renal nerve activity upon tubular sodium and water handling in the dog kidney was investigated. Following unilateral acute surgical denervation of the kidney a significant increase in urinary flow rate (40 +/- 7%), sodium clearance (26 +/- 4%), lithium clearance (9 +/- 2%) and fractional lithium clearance (8 +/- 2%) was seen, as compared to the contralateral kidney with preserved innervation. Calculated absolute proximal reabsorption rate decreased significantly by 7 +/- 2%, while calculated absolute rates of distal reabsorption of sodium and water increased significantly by 9 +/- 2% and 8 +/- 2%. Low-frequency electrical stimulation of the distal nerve bundle of the denervated kidney caused a significant decrease in urine flow rate (37 +/- 6%), sodium clearance (31 +/- 4%), lithium clearance (17 +/- 5%) and in fractional lithium clearance (18 +/- 5%). Calculated absolute proximal reabsorption rate increased significantly by 17 +/- 3%, while calculated absolute rates of distal sodium and water reabsorption decreased significantly by 16 +/- 5% and 16 +/- 5%. These changes in tubular sodium and water reabsorption during alterations in renal nerve activity occurred without measurable changes in renal blood flow (RBF) and glomerular filtration rate (GFR). Administration of amiloride had no significant effect either on the lithium clearance, RBF or GFR, while the sodium excretion rate increased and potassium excretion rate decreased, supporting that significant distal lithium reabsorption did not occur under the present experimental conditions.(ABSTRACT TRUNCATED AT 250 WORDS)",

author = "U Abildgaard and Holstein-Rathlou, {N H} and Leyssac, {P P}",

note = "Keywords: Amiloride; Animals; Body Water; Denervation; Dogs; Electric Stimulation; Glomerular Filtration Rate; Hemodynamics; Kidney; Kidney Tubules; Lithium; Renal Circulation; Sodium",

year = "1986",

language = "English",

volume = "126",

pages = "251--7",

journal = "Acta Physiologica",

issn = "1748-1708",

publisher = "Wiley-Blackwell",

number = "2",

}

TY - JOUR

T1 - Effect of renal nerve activity on tubular sodium and water reabsorption in dog kidneys as determined by the lithium clearance method.

AU - Abildgaard, U

AU - Holstein-Rathlou, N H

AU - Leyssac, P P

N1 - Keywords: Amiloride; Animals; Body Water; Denervation; Dogs; Electric Stimulation; Glomerular Filtration Rate; Hemodynamics; Kidney; Kidney Tubules; Lithium; Renal Circulation; Sodium

PY - 1986

Y1 - 1986

N2 - The reliability of the lithium clearance method in studies of the effect of renal nerve activity upon tubular sodium and water handling in the dog kidney was investigated. Following unilateral acute surgical denervation of the kidney a significant increase in urinary flow rate (40 +/- 7%), sodium clearance (26 +/- 4%), lithium clearance (9 +/- 2%) and fractional lithium clearance (8 +/- 2%) was seen, as compared to the contralateral kidney with preserved innervation. Calculated absolute proximal reabsorption rate decreased significantly by 7 +/- 2%, while calculated absolute rates of distal reabsorption of sodium and water increased significantly by 9 +/- 2% and 8 +/- 2%. Low-frequency electrical stimulation of the distal nerve bundle of the denervated kidney caused a significant decrease in urine flow rate (37 +/- 6%), sodium clearance (31 +/- 4%), lithium clearance (17 +/- 5%) and in fractional lithium clearance (18 +/- 5%). Calculated absolute proximal reabsorption rate increased significantly by 17 +/- 3%, while calculated absolute rates of distal sodium and water reabsorption decreased significantly by 16 +/- 5% and 16 +/- 5%. These changes in tubular sodium and water reabsorption during alterations in renal nerve activity occurred without measurable changes in renal blood flow (RBF) and glomerular filtration rate (GFR). Administration of amiloride had no significant effect either on the lithium clearance, RBF or GFR, while the sodium excretion rate increased and potassium excretion rate decreased, supporting that significant distal lithium reabsorption did not occur under the present experimental conditions.(ABSTRACT TRUNCATED AT 250 WORDS)

AB - The reliability of the lithium clearance method in studies of the effect of renal nerve activity upon tubular sodium and water handling in the dog kidney was investigated. Following unilateral acute surgical denervation of the kidney a significant increase in urinary flow rate (40 +/- 7%), sodium clearance (26 +/- 4%), lithium clearance (9 +/- 2%) and fractional lithium clearance (8 +/- 2%) was seen, as compared to the contralateral kidney with preserved innervation. Calculated absolute proximal reabsorption rate decreased significantly by 7 +/- 2%, while calculated absolute rates of distal reabsorption of sodium and water increased significantly by 9 +/- 2% and 8 +/- 2%. Low-frequency electrical stimulation of the distal nerve bundle of the denervated kidney caused a significant decrease in urine flow rate (37 +/- 6%), sodium clearance (31 +/- 4%), lithium clearance (17 +/- 5%) and in fractional lithium clearance (18 +/- 5%). Calculated absolute proximal reabsorption rate increased significantly by 17 +/- 3%, while calculated absolute rates of distal sodium and water reabsorption decreased significantly by 16 +/- 5% and 16 +/- 5%. These changes in tubular sodium and water reabsorption during alterations in renal nerve activity occurred without measurable changes in renal blood flow (RBF) and glomerular filtration rate (GFR). Administration of amiloride had no significant effect either on the lithium clearance, RBF or GFR, while the sodium excretion rate increased and potassium excretion rate decreased, supporting that significant distal lithium reabsorption did not occur under the present experimental conditions.(ABSTRACT TRUNCATED AT 250 WORDS)

M3 - Journal article

C2 - 3705985

VL - 126

SP - 251

EP - 257

JO - Acta Physiologica

JF - Acta Physiologica

SN - 1748-1708

IS - 2

ER -