Abstract
| Originalsprog | Engelsk |
|---|---|
| Tidsskrift | Nucleic Acids Research |
| Vol/bind | 25 |
| Udgave nummer | 17 |
| Sider (fra-til) | 3497-3502 |
| ISSN | 0305-1048 |
| DOI | |
| Status | Udgivet - 1997 |
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Effects of diaminopurine and inosine substitutions on A-tract induced DNA curvature. Importance of the 3'-Atract junction. / Møllegaard, Niels Erik; Bailly, Christian; Waring, Michael J.; Nielsen, Peter Eigil.
I: Nucleic Acids Research, Bind 25, Nr. 17, 1997, s. 3497-3502.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › peer review
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TY - JOUR
T1 - Effects of diaminopurine and inosine substitutions on A-tract induced DNA curvature. Importance of the 3'-Atract junction
AU - Møllegaard, Niels Erik
AU - Bailly, Christian
AU - Waring, Michael J.
AU - Nielsen, Peter Eigil
PY - 1997
Y1 - 1997
N2 - Gel migration and uranyl photoprobing have been used to study the effects of inosine and 2,6-diaminopurine (2,6-DAP) substitution on adenine-tract (A-tract) induced DNA curvature. Using a 10mer repeated sequence including five inosines we show by uranyl photoprobing that a narrow minor groove varying in phase with the helix repeat is not the cause of DNA curvature. Further, we have systematically studied by gel migration the effects on A-tract induced curvature of either single or full substitution with inosine and/or 2,6-DAP in a 5'-AAAAAGCCGC-3'sequence. DNA curvature is shown to increase when inosines are substituted for the guanosines in the sequence between the A-tracts. By comparing the effects of each monosubstitution it can be seen that when the G closest to the 3'-end of the A-tract is substituted the effect on DNA curvature is much stronger than when substitution is made at any other position. By contrast, curvature is abolished when 2,6-DAP residues are substituted for all adenines, and monosubstitution reveals that the effect of substituting a single adenine is strongest at the 3'-end of the A-tract. These results favor a model in which the curvature induced by an A-tract in DNA molecules is primarily located at the junction with the 3'-end of the A-tract, and this peculiar junction is created because the A-tract has a preference to form a non-B-DNA structure which builds up from the 5'-end.
AB - Gel migration and uranyl photoprobing have been used to study the effects of inosine and 2,6-diaminopurine (2,6-DAP) substitution on adenine-tract (A-tract) induced DNA curvature. Using a 10mer repeated sequence including five inosines we show by uranyl photoprobing that a narrow minor groove varying in phase with the helix repeat is not the cause of DNA curvature. Further, we have systematically studied by gel migration the effects on A-tract induced curvature of either single or full substitution with inosine and/or 2,6-DAP in a 5'-AAAAAGCCGC-3'sequence. DNA curvature is shown to increase when inosines are substituted for the guanosines in the sequence between the A-tracts. By comparing the effects of each monosubstitution it can be seen that when the G closest to the 3'-end of the A-tract is substituted the effect on DNA curvature is much stronger than when substitution is made at any other position. By contrast, curvature is abolished when 2,6-DAP residues are substituted for all adenines, and monosubstitution reveals that the effect of substituting a single adenine is strongest at the 3'-end of the A-tract. These results favor a model in which the curvature induced by an A-tract in DNA molecules is primarily located at the junction with the 3'-end of the A-tract, and this peculiar junction is created because the A-tract has a preference to form a non-B-DNA structure which builds up from the 5'-end.
U2 - 10.1093/nar/25.17.3497
DO - 10.1093/nar/25.17.3497
M3 - Journal article
VL - 25
SP - 3497
EP - 3502
JO - Nucleic Acids Research
JF - Nucleic Acids Research
SN - 0305-1048
IS - 17
ER -