@article{8511e2d0a02c11dd86a6000ea68e967b,
title = "Baculovirus-expressed constructs induce immunoglobulin G that recognizes VAR2CSA on Plasmodium falciparum-infected erythrocytes",
abstract = "We raised specific antisera against recombinant VAR2CSA domains produced in Escherichia coli and in insect cells. All were reactive in enzyme-linked immunosorbent assay, but only insect cell-derived constructs induced immunoglobulin G (IgG) that was reactive with native VAR2CSA on the surface of infected erythrocytes. Our data show that five of the six VAR2CSA Duffy-binding-like domains are surface exposed and that induction of surface-reactive VAR2CSA-specific IgG depends critically upon antigen conformation. These findings have implications for the development of vaccines against pregnancy-associated Plasmodium falciparum malaria.",
author = "Lea Barfod and Nielsen, {Morten A} and Louise Turner and Madeleine Dahlb{\"a}ck and Jensen, {Anja T R} and Lars Hviid and Theander, {Thor G} and Ali Salanti",
note = "Keywords: Animals; Antigens, Protozoan; Baculoviridae; Erythrocytes; Female; Genetic Vectors; Humans; Immune Sera; Immunoglobulin G; Mice; Plasmodium falciparum; Rabbits; Recombinant Proteins",
year = "2006",
doi = "10.1128/IAI.01617-05",
language = "English",
volume = "74",
pages = "4357--60",
journal = "Infection and Immunity",
issn = "0019-9567",
publisher = "American Society for Microbiology",
number = "7",
}