In vitro Leishmania major promastigote-induced macrophage migration is modulated by sensory and autonomic neuropeptides

A A Ahmed; A Wahbi; K Nordlind; A Kharazmi; K G Sundqvist; V Mutt; S Lidén

In vitro Leishmania major promastigote-induced macrophage migration is modulated by sensory and autonomic neuropeptides

A A Ahmed, A Wahbi, K Nordlind, A Kharazmi, K G Sundqvist, V Mutt, S Lidén

Centre for Medical Parasitology

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › peer review

40 Citationer (Scopus)

Abstract

Udgivelsesdato: 1998-Jul

Originalsprog	Engelsk
Tidsskrift	Scandinavian Journal of Immunology
Vol/bind	48
Udgave nummer	1
Sider (fra-til)	79-85
Antal sider	6
ISSN	0300-9475
Status	Udgivet - 1998

Bibliografisk note

Keywords: Animals; Autonomic Pathways; Calcitonin Gene-Related Peptide; Cell Line, Transformed; Chemotaxis; Leishmania major; Macrophages; Mice; Neurons, Afferent; Neuropeptide Y; Neuropeptides; Somatostatin; Substance P; Vasoactive Intestinal Peptide

Citationsformater

@article{8eb3adf01bcd11df8ed1000ea68e967b,

title = "In vitro Leishmania major promastigote-induced macrophage migration is modulated by sensory and autonomic neuropeptides",

abstract = "Recruitment, migration and adherence of macrophages and their interaction with inoculated promastigotes are key steps in the initiation of the inflammatory process in cutaneous leishmaniasis. Parasite- and nervous system-derived factors might be involved in this process. In the present study the chemotactic activities of live, killed and sonicated Leishmania major promastigotes and of the promastigote culture supernatant as well as the L. major surface protease gp63 towards a murine macrophage cell line, Raw 264.7, were investigated, using the Boyden technique. The sensory neuropeptides SOM, CGRP and SP, and the autonomic neuropeptides VIP and NPY, were also investigated for possible modulatory effects on this chemotaxis, using the living promastigotes. Living promastigotes were the most efficient attractants for macrophages compared with other forms of the parasites. Prior incubation of the macrophages with the parasites completely abolished the chemotactic activity. This might indicate that the living promastigote chemotaxis is a receptor-mediated process. On the other hand, paraformaldehyde-killed promastigotes not only failed to induce macrophage chemotaxis but also inhibited it in comparison with the control. The surface protease gp63 tended to inhibit the macrophage chemotactic activity and the sonicate tended to stimulate it compared with controls. The culture supernatant had no effect, indicating that the chemoattractive factors putatively synthesized by the living promastigotes are not released to the surrounding medium. Somatostatin inhibited L. major promastigote-induced macrophage migration at a high concentration, 10(-6) M, while substance P inhibited it at both low concentrations, 10(-10) and 10(-9) M, and a high one, 10(-6) M, the last-mentioned having the greatest inhibitory effect. A stimulatory effect of calcitonin gene-related peptide was found at high concentrations, 10(-5) and 10(-6) M. Vasoactive intestinal peptide stimulated macrophage chemotactic activity at both a high, 10(-5) M, and at a low, 10(-9) M, concentration, the same concentration at which neuropeptide Y exerted its maximum inhibitory effect.",

author = "Ahmed, {A A} and A Wahbi and K Nordlind and A Kharazmi and Sundqvist, {K G} and V Mutt and S Lid{\'e}n",

note = "Keywords: Animals; Autonomic Pathways; Calcitonin Gene-Related Peptide; Cell Line, Transformed; Chemotaxis; Leishmania major; Macrophages; Mice; Neurons, Afferent; Neuropeptide Y; Neuropeptides; Somatostatin; Substance P; Vasoactive Intestinal Peptide",

year = "1998",

language = "English",

volume = "48",

pages = "79--85",

journal = "Scandinavian Journal of Immunology",

issn = "0300-9475",

publisher = "Wiley-Blackwell",

number = "1",

}

TY - JOUR

T1 - In vitro Leishmania major promastigote-induced macrophage migration is modulated by sensory and autonomic neuropeptides

AU - Ahmed, A A

AU - Wahbi, A

AU - Nordlind, K

AU - Kharazmi, A

AU - Sundqvist, K G

AU - Mutt, V

AU - Lidén, S

N1 - Keywords: Animals; Autonomic Pathways; Calcitonin Gene-Related Peptide; Cell Line, Transformed; Chemotaxis; Leishmania major; Macrophages; Mice; Neurons, Afferent; Neuropeptide Y; Neuropeptides; Somatostatin; Substance P; Vasoactive Intestinal Peptide

PY - 1998

Y1 - 1998

N2 - Recruitment, migration and adherence of macrophages and their interaction with inoculated promastigotes are key steps in the initiation of the inflammatory process in cutaneous leishmaniasis. Parasite- and nervous system-derived factors might be involved in this process. In the present study the chemotactic activities of live, killed and sonicated Leishmania major promastigotes and of the promastigote culture supernatant as well as the L. major surface protease gp63 towards a murine macrophage cell line, Raw 264.7, were investigated, using the Boyden technique. The sensory neuropeptides SOM, CGRP and SP, and the autonomic neuropeptides VIP and NPY, were also investigated for possible modulatory effects on this chemotaxis, using the living promastigotes. Living promastigotes were the most efficient attractants for macrophages compared with other forms of the parasites. Prior incubation of the macrophages with the parasites completely abolished the chemotactic activity. This might indicate that the living promastigote chemotaxis is a receptor-mediated process. On the other hand, paraformaldehyde-killed promastigotes not only failed to induce macrophage chemotaxis but also inhibited it in comparison with the control. The surface protease gp63 tended to inhibit the macrophage chemotactic activity and the sonicate tended to stimulate it compared with controls. The culture supernatant had no effect, indicating that the chemoattractive factors putatively synthesized by the living promastigotes are not released to the surrounding medium. Somatostatin inhibited L. major promastigote-induced macrophage migration at a high concentration, 10(-6) M, while substance P inhibited it at both low concentrations, 10(-10) and 10(-9) M, and a high one, 10(-6) M, the last-mentioned having the greatest inhibitory effect. A stimulatory effect of calcitonin gene-related peptide was found at high concentrations, 10(-5) and 10(-6) M. Vasoactive intestinal peptide stimulated macrophage chemotactic activity at both a high, 10(-5) M, and at a low, 10(-9) M, concentration, the same concentration at which neuropeptide Y exerted its maximum inhibitory effect.

AB - Recruitment, migration and adherence of macrophages and their interaction with inoculated promastigotes are key steps in the initiation of the inflammatory process in cutaneous leishmaniasis. Parasite- and nervous system-derived factors might be involved in this process. In the present study the chemotactic activities of live, killed and sonicated Leishmania major promastigotes and of the promastigote culture supernatant as well as the L. major surface protease gp63 towards a murine macrophage cell line, Raw 264.7, were investigated, using the Boyden technique. The sensory neuropeptides SOM, CGRP and SP, and the autonomic neuropeptides VIP and NPY, were also investigated for possible modulatory effects on this chemotaxis, using the living promastigotes. Living promastigotes were the most efficient attractants for macrophages compared with other forms of the parasites. Prior incubation of the macrophages with the parasites completely abolished the chemotactic activity. This might indicate that the living promastigote chemotaxis is a receptor-mediated process. On the other hand, paraformaldehyde-killed promastigotes not only failed to induce macrophage chemotaxis but also inhibited it in comparison with the control. The surface protease gp63 tended to inhibit the macrophage chemotactic activity and the sonicate tended to stimulate it compared with controls. The culture supernatant had no effect, indicating that the chemoattractive factors putatively synthesized by the living promastigotes are not released to the surrounding medium. Somatostatin inhibited L. major promastigote-induced macrophage migration at a high concentration, 10(-6) M, while substance P inhibited it at both low concentrations, 10(-10) and 10(-9) M, and a high one, 10(-6) M, the last-mentioned having the greatest inhibitory effect. A stimulatory effect of calcitonin gene-related peptide was found at high concentrations, 10(-5) and 10(-6) M. Vasoactive intestinal peptide stimulated macrophage chemotactic activity at both a high, 10(-5) M, and at a low, 10(-9) M, concentration, the same concentration at which neuropeptide Y exerted its maximum inhibitory effect.

M3 - Journal article

C2 - 9714414

SN - 0300-9475

VL - 48

SP - 79

EP - 85

JO - Scandinavian Journal of Immunology

JF - Scandinavian Journal of Immunology

IS - 1

ER -