Engineering of a genetically encodable fluorescent voltage sensor exploiting fast Ci-VSP voltage-sensing movements

Alicia Lundby; Hiroki Mutoh; Dimitar Dimitrov; Walther Akemann; Thomas Knöpfel

doi:10.1371/journal.pone.0002514

Engineering of a genetically encodable fluorescent voltage sensor exploiting fast Ci-VSP voltage-sensing movements

Alicia Lundby, Hiroki Mutoh, Dimitar Dimitrov, Walther Akemann, Thomas Knöpfel

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › peer review

137 Citationer (Scopus)

Abstract

Udgivelsesdato: 2008

Originalsprog	Engelsk
Tidsskrift	PLoS ONE
Vol/bind	3
Udgave nummer	6
Sider (fra-til)	e2514
ISSN	1932-6203
DOI	https://doi.org/10.1371/journal.pone.0002514
Status	Udgivet - 2008

Bibliografisk note

Keywords: Animals; Fluorescence; Genetic Engineering; Ion Channel Gating; PC12 Cells; Potassium Channels; Protein Conformation; Rats

Adgang til dokumentet

10.1371/journal.pone.0002514

Citationsformater

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abstract = "Ci-VSP contains a voltage-sensing domain (VSD) homologous to that of voltage-gated potassium channels. Using charge displacement ('gating' current) measurements we show that voltage-sensing movements of this VSD can occur within 1 ms in mammalian membranes. Our analysis lead to development of a genetically encodable fluorescent protein voltage sensor (VSFP) in which the fast, voltage-dependent conformational changes of the Ci-VSP voltage sensor are transduced to similarly fast fluorescence read-outs.",

author = "Alicia Lundby and Hiroki Mutoh and Dimitar Dimitrov and Walther Akemann and Thomas Kn{\"o}pfel",

note = "Keywords: Animals; Fluorescence; Genetic Engineering; Ion Channel Gating; PC12 Cells; Potassium Channels; Protein Conformation; Rats",

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T1 - Engineering of a genetically encodable fluorescent voltage sensor exploiting fast Ci-VSP voltage-sensing movements

AU - Lundby, Alicia

AU - Mutoh, Hiroki

AU - Dimitrov, Dimitar

AU - Akemann, Walther

AU - Knöpfel, Thomas

N1 - Keywords: Animals; Fluorescence; Genetic Engineering; Ion Channel Gating; PC12 Cells; Potassium Channels; Protein Conformation; Rats

PY - 2008

Y1 - 2008

N2 - Ci-VSP contains a voltage-sensing domain (VSD) homologous to that of voltage-gated potassium channels. Using charge displacement ('gating' current) measurements we show that voltage-sensing movements of this VSD can occur within 1 ms in mammalian membranes. Our analysis lead to development of a genetically encodable fluorescent protein voltage sensor (VSFP) in which the fast, voltage-dependent conformational changes of the Ci-VSP voltage sensor are transduced to similarly fast fluorescence read-outs.

AB - Ci-VSP contains a voltage-sensing domain (VSD) homologous to that of voltage-gated potassium channels. Using charge displacement ('gating' current) measurements we show that voltage-sensing movements of this VSD can occur within 1 ms in mammalian membranes. Our analysis lead to development of a genetically encodable fluorescent protein voltage sensor (VSFP) in which the fast, voltage-dependent conformational changes of the Ci-VSP voltage sensor are transduced to similarly fast fluorescence read-outs.

U2 - 10.1371/journal.pone.0002514

DO - 10.1371/journal.pone.0002514

M3 - Journal article

C2 - 18575613

SN - 1932-6203

VL - 3

SP - e2514

JO - PLoS ONE

JF - PLoS ONE

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ER -