Enrichment of rosmarinic acid from comfrey (Symphytum officinale L.) root extract by macroporous adsorption resins and molecular docking studies

The rosmarinic acid (RA) is known as the major phenolic compound in comfrey roots possessing notable biological properties. This study aimed to enhance the polyphenol content of the comfrey root extract through the use of six different types of macroporous resins chromatography including DM-130, LXA-88, LSD-001, LXA-10, HP-20, and LXA-17. The LSD-001 demonstrated the highest separation efficiency, resulting in a 7-fold increase of RA content from 11.44 mg.g−1 in the extract to 75.79 mg.g−1 for the desorbed extract, as confirmed by HPLC analysis. In optimization of the static adsorption, 40 min contanct time, 11.8 g resin dosage, and 10000 µg mL−1 extract concentration were attained for maximum amount of adsorption capacity and ratio. Also in desorption process the minmum time for maximum recovery was 7 min with 60 mL eluent volume. The isotherm and kinetic studies of RA adsorption on LSD-001 resin showed reasonable agreement with Langmuir suggesting monolayer adsorption machenism, and the pseudo-second-order