@article{e15962f0439142d783b822298a3fe028,
title = "Enzyme-linked immunosorbent assay for activation of the classical complement pathway by Plasmodium falciparum-infected erythrocyte surface antigen-specific antibodies",
abstract = "Enzyme-linked immunosorbent assays (ELISA) have a wide range of applications, ranging from specific antibody titer determination to quantification of any biological or non-biological substance with a specific binding partner (usually an antibody). The activity of biological cascades, such as the complement cascade of the innate immune system, can also be assessed by ELISA. We present here an assay optimized for the detection of the activation of the classical complement pathway by polyclonal and monoclonal antibodies (mAbs) specific for Plasmodium falciparum-infected erythrocyte surface antigens.",
keywords = "Antibodies, Protozoan, Antigens, Protozoan, Antigens, Surface/metabolism, Complement Pathway, Classical, Enzyme-Linked Immunosorbent Assay, Erythrocytes/metabolism, Humans, Malaria, Falciparum, Plasmodium falciparum/metabolism, Protozoan Proteins/metabolism",
author = "Larsen, {Mads Delbo} and Rafael Bayarri-Olmos and Peter Garred and Lars Hviid",
note = "{\textcopyright} 2022. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.",
year = "2022",
doi = "10.1007/978-1-0716-2189-9_50",
language = "English",
volume = "2470",
pages = "673--678",
journal = "Methods in Molecular Biology",
issn = "1064-3745",
publisher = "Humana Press",
}