Evaluation of simple tests for detection of HIV antibodies: analysis of interobserver variation in Tanzania

A Jørgensen; Jing Shao; S Maselle; E Yangi; A Thomsen; S Matunda; Ib Christian Bygbjerg; P Gøtzsche; J Svendsen; null Skinhøj

Evaluation of simple tests for detection of HIV antibodies: analysis of interobserver variation in Tanzania

A Jørgensen, Jing Shao, S Maselle, E Yangi, A Thomsen, S Matunda, Ib Christian Bygbjerg, P Gøtzsche, J Svendsen, Skinhøj

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › peer review

2 Citationer (Scopus)

Abstract

200 sera were tested for HIV antibodies with different tests in Tanzania. The results were interpreted by 5 different observers with different laboratory experience. There was considerable variation between observers and between testing methods. HIV-Chek was easiest to perform with little interobserver variation, but a few probably false negative readings were seen. Serodia gave many false positives. Western Blots gave more diverging results due to indeterminate sera and lack of training. HIV-Chek seems best when time, facilities, and training are limited and if combined with Serodia false negative results can be excluded.

Originalsprog	Engelsk
Tidsskrift	Scandinavian Journal of Infectious Diseases
Vol/bind	22
Udgave nummer	3
Sider (fra-til)	283-5
Antal sider	3
ISSN	0036-5548
Status	Udgivet - 1 jan. 1990

Citationsformater

@article{e0da92fd2b73488c94d12e74dd94bc04,

title = "Evaluation of simple tests for detection of HIV antibodies: analysis of interobserver variation in Tanzania",

abstract = "200 sera were tested for HIV antibodies with different tests in Tanzania. The results were interpreted by 5 different observers with different laboratory experience. There was considerable variation between observers and between testing methods. HIV-Chek was easiest to perform with little interobserver variation, but a few probably false negative readings were seen. Serodia gave many false positives. Western Blots gave more diverging results due to indeterminate sera and lack of training. HIV-Chek seems best when time, facilities, and training are limited and if combined with Serodia false negative results can be excluded.",

keywords = "Blotting, Western, Enzyme-Linked Immunosorbent Assay, False Negative Reactions, False Positive Reactions, HIV Antibodies, HIV Infections, Humans, Observer Variation, Predictive Value of Tests, Tanzania",

author = "A J{\o}rgensen and Jing Shao and S Maselle and E Yangi and A Thomsen and S Matunda and Bygbjerg, {Ib Christian} and P G{\o}tzsche and J Svendsen and Skinh{\o}j",

year = "1990",

month = jan,

day = "1",

language = "English",

volume = "22",

pages = "283--5",

journal = "Scandinavian Journal of Infectious Diseases",

issn = "0036-5548",

publisher = "Taylor & Francis",

number = "3",

}

TY - JOUR

T1 - Evaluation of simple tests for detection of HIV antibodies: analysis of interobserver variation in Tanzania

AU - Jørgensen, A

AU - Shao, Jing

AU - Maselle, S

AU - Yangi, E

AU - Thomsen, A

AU - Matunda, S

AU - Bygbjerg, Ib Christian

AU - Gøtzsche, P

AU - Svendsen, J

AU - Skinhøj, null

PY - 1990/1/1

Y1 - 1990/1/1

N2 - 200 sera were tested for HIV antibodies with different tests in Tanzania. The results were interpreted by 5 different observers with different laboratory experience. There was considerable variation between observers and between testing methods. HIV-Chek was easiest to perform with little interobserver variation, but a few probably false negative readings were seen. Serodia gave many false positives. Western Blots gave more diverging results due to indeterminate sera and lack of training. HIV-Chek seems best when time, facilities, and training are limited and if combined with Serodia false negative results can be excluded.

AB - 200 sera were tested for HIV antibodies with different tests in Tanzania. The results were interpreted by 5 different observers with different laboratory experience. There was considerable variation between observers and between testing methods. HIV-Chek was easiest to perform with little interobserver variation, but a few probably false negative readings were seen. Serodia gave many false positives. Western Blots gave more diverging results due to indeterminate sera and lack of training. HIV-Chek seems best when time, facilities, and training are limited and if combined with Serodia false negative results can be excluded.

KW - Blotting, Western

KW - Enzyme-Linked Immunosorbent Assay

KW - False Negative Reactions

KW - False Positive Reactions

KW - HIV Antibodies

KW - HIV Infections

KW - Humans

KW - Observer Variation

KW - Predictive Value of Tests

KW - Tanzania

M3 - Journal article

C2 - 2371543

SN - 0036-5548

VL - 22

SP - 283

EP - 285

JO - Scandinavian Journal of Infectious Diseases

JF - Scandinavian Journal of Infectious Diseases

IS - 3

ER -