Exercise induces expression of leukaemia inhibitory factor in human skeletal muscle.

Christa Broholm; Ole Hartvig Mortensen; Søren Nielsen; Thorbjörn Åkerström; Alaa Zankari; Benny Dahl; Bente Klarlund Pedersen

doi:10.1113/jphysiol.2007.149781

Exercise induces expression of leukaemia inhibitory factor in human skeletal muscle.

Christa Broholm, Ole Hartvig Mortensen, Søren Nielsen, Thorbjörn Åkerström, Alaa Zankari, Benny Dahl, Bente Klarlund Pedersen

Inflammation, Metabolism and Oxidation

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › peer review

107 Citationer (Scopus)

Abstract

Udgivelsesdato: 2008-Apr-15

Originalsprog	Engelsk
Tidsskrift	Journal of Physiology
Vol/bind	586
Udgave nummer	8
Sider (fra-til)	2195-201
Antal sider	6
ISSN	0022-3751
DOI	https://doi.org/10.1113/jphysiol.2007.149781
Status	Udgivet - 2008

Bibliografisk note

Keywords: Adult; Cells, Cultured; Exertion; Gene Expression Regulation; Humans; Leukemia Inhibitory Factor; Male; Muscle Contraction; Muscle Fibers; Muscle, Skeletal; Physical Endurance

Adgang til dokumentet

10.1113/jphysiol.2007.149781

Citationsformater

@article{91a1f450acd311ddb538000ea68e967b,

title = "Exercise induces expression of leukaemia inhibitory factor in human skeletal muscle.",

abstract = "The leukaemia inhibitory factor (LIF) belongs to the interleukin (IL)-6 cytokine superfamily and is constitutively expressed in skeletal muscle. We tested the hypothesis that LIF expression in human skeletal muscle is regulated by exercise. Fifteen healthy young male volunteers performed either 3 h of cycle ergometer exercise at approximately 60% of VO2,max(n = 8) or rested (n = 7). Muscle biopsies were obtained from the vastus lateralis prior to exercise, immediately after exercise, and at 1.5, 3, 6 and 24 h post exercise. Control subjects had biopsy samples taken at the same time points as during the exercise trial. Skeletal muscle LIF mRNA increased immediately after the exercise and declined gradually during recovery. However, LIF protein was unchanged at the investigated time points. Moreover, we tested the hypothesis that LIF mRNA and protein expressions are modulated by calcium (Ca(2+)) in primary human skeletal myocytes. Treatment of myocytes with the Ca(2+) ionophore, ionomycin, for 6 h resulted in an increase in both LIF mRNA and LIF protein levels. This finding suggests that Ca(2+) may be involved in the regulation of LIF in endurance-exercised skeletal muscle. In conclusion, primary human skeletal myocytes have the capability to produce LIF in response to ionomycin stimulation and LIF mRNA levels increase in skeletal muscle following concentric exercise. The finding that the increase in LIF mRNA levels is not followed by a similar increase in skeletal muscle LIF protein suggests that other exercise stimuli or repetitive stimuli are necessary in order to induce a detectable accumulation of LIF protein.",

author = "Christa Broholm and Mortensen, {Ole Hartvig} and S{\o}ren Nielsen and Thorbj{\"o}rn {\AA}kerstr{\"o}m and Alaa Zankari and Benny Dahl and Pedersen, {Bente Klarlund}",

note = "Keywords: Adult; Cells, Cultured; Exertion; Gene Expression Regulation; Humans; Leukemia Inhibitory Factor; Male; Muscle Contraction; Muscle Fibers; Muscle, Skeletal; Physical Endurance",

year = "2008",

doi = "10.1113/jphysiol.2007.149781",

language = "English",

volume = "586",

pages = "2195--201",

journal = "Journal of Physiology",

issn = "0022-3751",

publisher = "Wiley-Blackwell",

number = "8",

}

TY - JOUR

T1 - Exercise induces expression of leukaemia inhibitory factor in human skeletal muscle.

AU - Broholm, Christa

AU - Mortensen, Ole Hartvig

AU - Nielsen, Søren

AU - Åkerström, Thorbjörn

AU - Zankari, Alaa

AU - Dahl, Benny

AU - Pedersen, Bente Klarlund

N1 - Keywords: Adult; Cells, Cultured; Exertion; Gene Expression Regulation; Humans; Leukemia Inhibitory Factor; Male; Muscle Contraction; Muscle Fibers; Muscle, Skeletal; Physical Endurance

PY - 2008

Y1 - 2008

N2 - The leukaemia inhibitory factor (LIF) belongs to the interleukin (IL)-6 cytokine superfamily and is constitutively expressed in skeletal muscle. We tested the hypothesis that LIF expression in human skeletal muscle is regulated by exercise. Fifteen healthy young male volunteers performed either 3 h of cycle ergometer exercise at approximately 60% of VO2,max(n = 8) or rested (n = 7). Muscle biopsies were obtained from the vastus lateralis prior to exercise, immediately after exercise, and at 1.5, 3, 6 and 24 h post exercise. Control subjects had biopsy samples taken at the same time points as during the exercise trial. Skeletal muscle LIF mRNA increased immediately after the exercise and declined gradually during recovery. However, LIF protein was unchanged at the investigated time points. Moreover, we tested the hypothesis that LIF mRNA and protein expressions are modulated by calcium (Ca(2+)) in primary human skeletal myocytes. Treatment of myocytes with the Ca(2+) ionophore, ionomycin, for 6 h resulted in an increase in both LIF mRNA and LIF protein levels. This finding suggests that Ca(2+) may be involved in the regulation of LIF in endurance-exercised skeletal muscle. In conclusion, primary human skeletal myocytes have the capability to produce LIF in response to ionomycin stimulation and LIF mRNA levels increase in skeletal muscle following concentric exercise. The finding that the increase in LIF mRNA levels is not followed by a similar increase in skeletal muscle LIF protein suggests that other exercise stimuli or repetitive stimuli are necessary in order to induce a detectable accumulation of LIF protein.

AB - The leukaemia inhibitory factor (LIF) belongs to the interleukin (IL)-6 cytokine superfamily and is constitutively expressed in skeletal muscle. We tested the hypothesis that LIF expression in human skeletal muscle is regulated by exercise. Fifteen healthy young male volunteers performed either 3 h of cycle ergometer exercise at approximately 60% of VO2,max(n = 8) or rested (n = 7). Muscle biopsies were obtained from the vastus lateralis prior to exercise, immediately after exercise, and at 1.5, 3, 6 and 24 h post exercise. Control subjects had biopsy samples taken at the same time points as during the exercise trial. Skeletal muscle LIF mRNA increased immediately after the exercise and declined gradually during recovery. However, LIF protein was unchanged at the investigated time points. Moreover, we tested the hypothesis that LIF mRNA and protein expressions are modulated by calcium (Ca(2+)) in primary human skeletal myocytes. Treatment of myocytes with the Ca(2+) ionophore, ionomycin, for 6 h resulted in an increase in both LIF mRNA and LIF protein levels. This finding suggests that Ca(2+) may be involved in the regulation of LIF in endurance-exercised skeletal muscle. In conclusion, primary human skeletal myocytes have the capability to produce LIF in response to ionomycin stimulation and LIF mRNA levels increase in skeletal muscle following concentric exercise. The finding that the increase in LIF mRNA levels is not followed by a similar increase in skeletal muscle LIF protein suggests that other exercise stimuli or repetitive stimuli are necessary in order to induce a detectable accumulation of LIF protein.

U2 - 10.1113/jphysiol.2007.149781

DO - 10.1113/jphysiol.2007.149781

M3 - Journal article

C2 - 18292129

SN - 0022-3751

VL - 586

SP - 2195

EP - 2201

JO - Journal of Physiology

JF - Journal of Physiology

IS - 8

ER -