TY - JOUR
T1 - Exit from mitosis triggers Chs2p transport from the endoplasmic reticulum to mother-daughter neck via the secretory pathway in budding yeast
AU - Zhang, Gang
AU - Kashimshetty, Rohini
AU - Ng, Kwee Eng
AU - Tan, Heng Buck
AU - Yeong, Foong May
N1 - Keywords: Cell Cycle Proteins; Chitin; Chitin Synthase; Endoplasmic Reticulum; GTP-Binding Proteins; Mitosis; Mutation; Phosphotransferases; Protein Transport; Recombinant Fusion Proteins; Saccharomyces cerevisiae Proteins; Saccharomycetales; Telophase; beta-Fructofuranosidase
PY - 2006
Y1 - 2006
N2 - Budding yeast chitin synthase 2 (Chs2p), which lays down the primary septum, localizes to the mother-daughter neck in telophase. However, the mechanism underlying the timely neck localization of Chs2p is not known. Recently, it was found that a component of the exocyst complex, Sec3p-green fluorescent protein, arrives at the neck upon mitotic exit. It is not clear whether the neck localization of Chs2p, which is a cargo of the exocyst complex, was similarly regulated by mitotic exit. We report that Chs2p was restrained in the endoplasmic reticulum (ER) during metaphase. Furthermore, mitotic exit was sufficient to cause Chs2p neck localization specifically by triggering the Sec12p-dependent transport of Chs2p out of the ER. Chs2p was "forced" prematurely to the neck by mitotic kinase inactivation at metaphase, with chitin deposition occurring between mother and daughter cells. The dependence of Chs2p exit from the ER followed by its transport to the neck upon mitotic exit ensures that septum formation occurs only after the completion of mitotic events.
AB - Budding yeast chitin synthase 2 (Chs2p), which lays down the primary septum, localizes to the mother-daughter neck in telophase. However, the mechanism underlying the timely neck localization of Chs2p is not known. Recently, it was found that a component of the exocyst complex, Sec3p-green fluorescent protein, arrives at the neck upon mitotic exit. It is not clear whether the neck localization of Chs2p, which is a cargo of the exocyst complex, was similarly regulated by mitotic exit. We report that Chs2p was restrained in the endoplasmic reticulum (ER) during metaphase. Furthermore, mitotic exit was sufficient to cause Chs2p neck localization specifically by triggering the Sec12p-dependent transport of Chs2p out of the ER. Chs2p was "forced" prematurely to the neck by mitotic kinase inactivation at metaphase, with chitin deposition occurring between mother and daughter cells. The dependence of Chs2p exit from the ER followed by its transport to the neck upon mitotic exit ensures that septum formation occurs only after the completion of mitotic events.
U2 - 10.1083/jcb.200604094
DO - 10.1083/jcb.200604094
M3 - Journal article
C2 - 16847101
SN - 0021-9525
VL - 174
SP - 207
EP - 220
JO - Journal of Cell Biology
JF - Journal of Cell Biology
IS - 2
ER -