Abstract
In severe malformations with a lack of native tissues, treatment options are limited. We aimed at expanding tissue in vivo using the body as a bioreactor and developing a sustainable single-staged procedure for autologous tissue reconstruction in malformation surgery. Autologous micro-epithelium from skin was integrated with plastically compressed collagen and a degradable knitted fabric mesh. Sixty-three scaffolds were implanted in nine rats for histological and mechanical analyses, up to 4 weeks after transplantation. Tissue integration, cell expansion, proliferation, inflammation, strength, and elasticity were evaluated over time in vivo and validated in vitro in a bladder wound healing model. After 5 days in vivo, we observed keratinocyte proliferation on top of the transplant, remodeling of the collagen, and neovascularization within the transplant. At 4 weeks, all transplants were fully integrated with the surrounding tissue. Tensile strength and elasticity were retained during the whole study period. In the in vitro models, a multilayered epithelium covered the defect after 4 weeks. Autologous micro-epithelial transplants allowed for cell expansion and reorganization in vivo without conventional pre-operative in vitro cell propagation. The method was easy to perform and did not require handling outside the operating theater.
Originalsprog | Engelsk |
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Artikelnummer | 12703 |
Tidsskrift | International Journal of Molecular Sciences |
Vol/bind | 23 |
Udgave nummer | 20 |
ISSN | 1661-6596 |
DOI | |
Status | Udgivet - 2022 |
Bibliografisk note
Funding Information:This research was funded by The Swedish Society of Medical Research, The Stockholm City Council, HRH Crown princess Lovisa’s Memorial Foundation, The Childcare Association at Karolinska Institutet, The Samariten Foundation, Freemason’s Fund for Children’s Health, and the Novo Nordisk Foundation NNFSA170030576.
Publisher Copyright:
© 2022 by the authors.