Expression and secretion of a lytic polysaccharide monooxygenase by a fast-growing cyanobacterium

D. A. Russo; J. A. Z. Zedler; D. N. Wittmann; B. Möllers; R. K. Singh; T. S. Batth; B. van Oort; J. V. Olsen; M. J. Bjerrum; P. E. Jensen

doi:10.1186/s13068-019-1416-9

Expression and secretion of a lytic polysaccharide monooxygenase by a fast-growing cyanobacterium

D. A. Russo, J. A. Z. Zedler, D. N. Wittmann, B. Möllers, R. K. Singh, T. S. Batth, B. van Oort, J. V. Olsen, M. J. Bjerrum, P. E. Jensen

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › peer review

32 Citationer (Scopus)

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Abstract

Cyanobacteria have the potential to become next-generation cell factories due to their ability to use CO2, light and inorganic nutrients to produce a range of biomolecules of commercial interest. Synechococcus elongatus UTEX 2973, in particular, is a fast-growing, genetically tractable, cyanobacterium that has garnered attention as a potential biotechnological chassis. To establish this unique strain as a host for heterologous protein production, we aimed to demonstrate expression and secretion of the industrially relevant TfAA10A, a lytic polysaccharide monooxygenase from the Gram-positive bacterium Thermobifida fusca.

Originalsprog	Engelsk
Artikelnummer	74
Tidsskrift	Biotechnology for Biofuels
Vol/bind	12
Udgave nummer	1
Antal sider	13
ISSN	1754-6834
DOI	https://doi.org/10.1186/s13068-019-1416-9
Status	Udgivet - 2019

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10.1186/s13068-019-1416-9Licens: CC BY

Expression and secretion of a lytic polysaccharide monooxygenase by a fast‑growing cyanobacteriumForlagets udgivne version, 1,15 MBLicens: CC BY

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title = "Expression and secretion of a lytic polysaccharide monooxygenase by a fast-growing cyanobacterium",

abstract = "Cyanobacteria have the potential to become next-generation cell factories due to their ability to use CO2, light and inorganic nutrients to produce a range of biomolecules of commercial interest. Synechococcus elongatus UTEX 2973, in particular, is a fast-growing, genetically tractable, cyanobacterium that has garnered attention as a potential biotechnological chassis. To establish this unique strain as a host for heterologous protein production, we aimed to demonstrate expression and secretion of the industrially relevant TfAA10A, a lytic polysaccharide monooxygenase from the Gram-positive bacterium Thermobifida fusca.",

author = "Russo, {D. A.} and Zedler, {J. A. Z.} and Wittmann, {D. N.} and B. M{\"o}llers and Singh, {R. K.} and Batth, {T. S.} and {van Oort}, B. and Olsen, {J. V.} and Bjerrum, {M. J.} and Jensen, {P. E.}",

year = "2019",

doi = "10.1186/s13068-019-1416-9",

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TY - JOUR

T1 - Expression and secretion of a lytic polysaccharide monooxygenase by a fast-growing cyanobacterium

AU - Russo, D. A.

AU - Zedler, J. A. Z.

AU - Wittmann, D. N.

AU - Möllers, B.

AU - Singh, R. K.

AU - Batth, T. S.

AU - van Oort, B.

AU - Olsen, J. V.

AU - Bjerrum, M. J.

AU - Jensen, P. E.

PY - 2019

Y1 - 2019

N2 - Cyanobacteria have the potential to become next-generation cell factories due to their ability to use CO2, light and inorganic nutrients to produce a range of biomolecules of commercial interest. Synechococcus elongatus UTEX 2973, in particular, is a fast-growing, genetically tractable, cyanobacterium that has garnered attention as a potential biotechnological chassis. To establish this unique strain as a host for heterologous protein production, we aimed to demonstrate expression and secretion of the industrially relevant TfAA10A, a lytic polysaccharide monooxygenase from the Gram-positive bacterium Thermobifida fusca.

AB - Cyanobacteria have the potential to become next-generation cell factories due to their ability to use CO2, light and inorganic nutrients to produce a range of biomolecules of commercial interest. Synechococcus elongatus UTEX 2973, in particular, is a fast-growing, genetically tractable, cyanobacterium that has garnered attention as a potential biotechnological chassis. To establish this unique strain as a host for heterologous protein production, we aimed to demonstrate expression and secretion of the industrially relevant TfAA10A, a lytic polysaccharide monooxygenase from the Gram-positive bacterium Thermobifida fusca.

U2 - 10.1186/s13068-019-1416-9

DO - 10.1186/s13068-019-1416-9

M3 - Journal article

C2 - 30976324

SN - 1754-6834

VL - 12

JO - Biotechnology for Biofuels

JF - Biotechnology for Biofuels

IS - 1

M1 - 74

ER -