TY - JOUR
T1 - Expression, crystallization and preliminary X-ray analysis of the extracellular Ig modules I-IV and F3 modules I-III of the neural cell-adhesion molecule L1
AU - Kulahin, Nikolaj
AU - Kasper, Christina
AU - Kristensen, Ole
AU - Kastrup, Jette Sandholm
AU - Berezin, Vladimir
AU - Bock, Elisabeth
AU - Gajhede, Michael
N1 - Keywords: Animals; Crystallization; Crystallography, X-Ray; Fibronectins; Gene Expression; Immunoglobulins; Mice; Neural Cell Adhesion Molecule L1
PY - 2005
Y1 - 2005
N2 - Four amino-terminal immunoglobulin (Ig) modules and three fibronectin type III (F3) modules of the mouse neural cell-adhesion molecule L1 have been expressed in Drosophila S2 cells. The Ig modules I-IV of L1 crystallized in a trigonal space group, with unit-cell parameters a = b = 239.6, c = 99.3 A, and the crystals diffracted X-rays to a resolution of about 3.5 A. The F3 modules I-III of L1 crystallized in a tetragonal space group, with unit-cell parameters a = b = 80.1, c = 131 A, and the crystals diffracted X-rays to 2.8 A resolution. This is a step towards the structure determination of the multimodular constructs of the neural cell-adhesion molecule L1 in order to understand the function of L1 on a structural basis.
AB - Four amino-terminal immunoglobulin (Ig) modules and three fibronectin type III (F3) modules of the mouse neural cell-adhesion molecule L1 have been expressed in Drosophila S2 cells. The Ig modules I-IV of L1 crystallized in a trigonal space group, with unit-cell parameters a = b = 239.6, c = 99.3 A, and the crystals diffracted X-rays to a resolution of about 3.5 A. The F3 modules I-III of L1 crystallized in a tetragonal space group, with unit-cell parameters a = b = 80.1, c = 131 A, and the crystals diffracted X-rays to 2.8 A resolution. This is a step towards the structure determination of the multimodular constructs of the neural cell-adhesion molecule L1 in order to understand the function of L1 on a structural basis.
U2 - 10.1107/S1744309105026783
DO - 10.1107/S1744309105026783
M3 - Journal article
C2 - 16511179
SN - 1744-3091
VL - 61
SP - 858
EP - 860
JO - Acta Crystallographica. Section F : Structural Biology and Crystallization Communications
JF - Acta Crystallographica. Section F : Structural Biology and Crystallization Communications
IS - Pt 9
ER -