Fate of circulating amino-terminal propeptide of type III procollagen in conscious pigs

L T Jensen; Jens Henrik Sahl Henriksen; J Risteli; H P Olesen; M D Nielsen; I Lorenzen

Fate of circulating amino-terminal propeptide of type III procollagen in conscious pigs

L T Jensen, Jens Henrik Sahl Henriksen, J Risteli, H P Olesen, M D Nielsen, I Lorenzen

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › peer review

15 Citationer (Scopus)

Abstract

Udgivelsesdato: 1993-Jul

Originalsprog	Engelsk
Tidsskrift	American Journal of Physiology (Consolidated)
Vol/bind	265
Udgave nummer	1 Pt 2
Sider (fra-til)	R139-45
ISSN	0002-9513
Status	Udgivet - 1993

Bibliografisk note

Keywords: Animals; Female; Humans; Lymph; Models, Biological; Peptide Fragments; Procollagen; Swine; Time Factors

Citationsformater

@article{7e42310031ab11df8ed1000ea68e967b,

title = "Fate of circulating amino-terminal propeptide of type III procollagen in conscious pigs",

abstract = "The amino-terminal propeptide of type III procollagen (PIIINP, M(r) 42,000) is a promising marker for the formation of type III collagen of granulation tissue in experimental and clinical studies. The disposal kinetics of circulating PIIINP is, however, almost unknown. In conscious pigs with a thoracic duct-venous shunt, 125I-labeled PIIINP was injected intravenously. The initial distribution volume was 2.2 liters, which was 1.7 times the plasma volume (P < 0.01). The disappearance curve was three-phased, with an initial steep decline (t1/2 58 min), followed by two slower phases (t1/2 239 min and 289 h). Consecutive gel filtrations showed that the initial slope of the plasma disappearance curve corresponded to the plasma clearance of the intact PIIINP. The initial plasma clearance was 26.5 ml plasma/min, whereas the urinary clearance was 8.7 ml plasma/min (P < 0.01). The other components of the plasma disappearance curve originated from the formation and disappearance of a high and a low molecular weight (MW) fraction as part of the degradation of PIIINP. The high MW fraction (approximately M(r) 90,000) was similar to a previously described, but not further characterized, PIIINP immunoreactive component. The existence of the low MW fraction (approximately M(r) 20,000) has not been reported before. The lymphatic recirculation of intact PIIINP was rapid, and the lymph-serum ratio was almost constant within 1 h of injection. We conclude that the t1/2 of circulating PIIINP is 58 min, that PIIINP escapes the circulation very quickly, and that the degradation of PIIINP includes at least two intermediary steps.",

author = "Jensen, {L T} and Henriksen, {Jens Henrik Sahl} and J Risteli and Olesen, {H P} and Nielsen, {M D} and I Lorenzen",

note = "Keywords: Animals; Female; Humans; Lymph; Models, Biological; Peptide Fragments; Procollagen; Swine; Time Factors",

year = "1993",

language = "English",

volume = "265",

pages = "R139--45",

journal = "American Journal of Physiology (Consolidated)",

issn = "0002-9513",

publisher = "American Physiological Society",

number = "1 Pt 2",

}

TY - JOUR

T1 - Fate of circulating amino-terminal propeptide of type III procollagen in conscious pigs

AU - Jensen, L T

AU - Henriksen, Jens Henrik Sahl

AU - Risteli, J

AU - Olesen, H P

AU - Nielsen, M D

AU - Lorenzen, I

N1 - Keywords: Animals; Female; Humans; Lymph; Models, Biological; Peptide Fragments; Procollagen; Swine; Time Factors

PY - 1993

Y1 - 1993

N2 - The amino-terminal propeptide of type III procollagen (PIIINP, M(r) 42,000) is a promising marker for the formation of type III collagen of granulation tissue in experimental and clinical studies. The disposal kinetics of circulating PIIINP is, however, almost unknown. In conscious pigs with a thoracic duct-venous shunt, 125I-labeled PIIINP was injected intravenously. The initial distribution volume was 2.2 liters, which was 1.7 times the plasma volume (P < 0.01). The disappearance curve was three-phased, with an initial steep decline (t1/2 58 min), followed by two slower phases (t1/2 239 min and 289 h). Consecutive gel filtrations showed that the initial slope of the plasma disappearance curve corresponded to the plasma clearance of the intact PIIINP. The initial plasma clearance was 26.5 ml plasma/min, whereas the urinary clearance was 8.7 ml plasma/min (P < 0.01). The other components of the plasma disappearance curve originated from the formation and disappearance of a high and a low molecular weight (MW) fraction as part of the degradation of PIIINP. The high MW fraction (approximately M(r) 90,000) was similar to a previously described, but not further characterized, PIIINP immunoreactive component. The existence of the low MW fraction (approximately M(r) 20,000) has not been reported before. The lymphatic recirculation of intact PIIINP was rapid, and the lymph-serum ratio was almost constant within 1 h of injection. We conclude that the t1/2 of circulating PIIINP is 58 min, that PIIINP escapes the circulation very quickly, and that the degradation of PIIINP includes at least two intermediary steps.

AB - The amino-terminal propeptide of type III procollagen (PIIINP, M(r) 42,000) is a promising marker for the formation of type III collagen of granulation tissue in experimental and clinical studies. The disposal kinetics of circulating PIIINP is, however, almost unknown. In conscious pigs with a thoracic duct-venous shunt, 125I-labeled PIIINP was injected intravenously. The initial distribution volume was 2.2 liters, which was 1.7 times the plasma volume (P < 0.01). The disappearance curve was three-phased, with an initial steep decline (t1/2 58 min), followed by two slower phases (t1/2 239 min and 289 h). Consecutive gel filtrations showed that the initial slope of the plasma disappearance curve corresponded to the plasma clearance of the intact PIIINP. The initial plasma clearance was 26.5 ml plasma/min, whereas the urinary clearance was 8.7 ml plasma/min (P < 0.01). The other components of the plasma disappearance curve originated from the formation and disappearance of a high and a low molecular weight (MW) fraction as part of the degradation of PIIINP. The high MW fraction (approximately M(r) 90,000) was similar to a previously described, but not further characterized, PIIINP immunoreactive component. The existence of the low MW fraction (approximately M(r) 20,000) has not been reported before. The lymphatic recirculation of intact PIIINP was rapid, and the lymph-serum ratio was almost constant within 1 h of injection. We conclude that the t1/2 of circulating PIIINP is 58 min, that PIIINP escapes the circulation very quickly, and that the degradation of PIIINP includes at least two intermediary steps.

M3 - Journal article

C2 - 8342678

SN - 0002-9513

VL - 265

SP - R139-45

JO - American Journal of Physiology (Consolidated)

JF - American Journal of Physiology (Consolidated)

IS - 1 Pt 2

ER -