Abstract
Adenosine bases of RNA can be transiently modified by the deposition of a methyl-group to form N6-methyladenosine (m6A). This adenosine-methylation is an ancient process and the enzymes involved are evolutionary highly conserved. A genetic screen designed to identify suppressors of late flowering transgenic Arabidopsis plants overexpressing the miP1a microProtein yielded a new allele of the FIONA1 (FIO1) m6A-methyltransferase. To characterize the early flowering phenotype of fio1 mutant plants we employed an integrative approach of mRNA-seq, Nanopore direct RNA-sequencing and meRIP-seq to identify differentially expressed transcripts as well as differentially methylated RNAs. We provide evidence that FIO1 is the elusive methyltransferase responsible for the 3'-end methylation of the FLOWERING LOCUS C (FLC) transcript. Furthermore, our genetic and biochemical data suggest that 3′-methylation stabilizes FLC mRNAs and non-methylated FLC is a target for rapid degradatio.
| Originalsprog | Engelsk |
|---|---|
| Artikelnummer | e1010386 |
| Tidsskrift | PLOS Genetics |
| Vol/bind | 18 |
| Udgave nummer | 9 |
| Antal sider | 22 |
| ISSN | 1553-7390 |
| DOI | |
| Status | Udgivet - 2022 |
Bibliografisk note
Funding Information:We acknowledge funding through NovoCrops Centre (Novo Nordisk Foundation project number 2019OC53580 to S. W.), the Independent Research Fund Denmark (0136- 00015B and 0135-00014B to S. W.), the Novo Nordisk Foundation (NNF18OC0034226 and NNF20OC0061440 to S. W.), and the funding of DataPLANT (NFDI 7/1 - 42077441) as part of the German National Research Data Infrastructure funded by the Deutsche Forschungsgemeinschaft (DFG - German Research Foundation). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
Publisher Copyright:
Copyright © 2022 Sun et al.