FLIPR assays of intracellular calcium in GPCR drug discovery

Kasper Bø Hansen, Hans Bräuner-Osborne

    Publikation: Bidrag til bog/antologi/rapportBidrag til bog/antologiForskning

    24 Citationer (Scopus)

    Abstract

    Fluorescent dyes sensitive to changes in intracellular calcium have become increasingly popular in G protein-coupled receptor (GPCR) drug discovery for several reasons. First of all, the assays using the dyes are easy to perform and are of low cost compared to other assays. Second, most non-Galpha(q)-coupled GPCRs can be tweaked to modulate intracellular calcium by co-transfection with promiscuous or chimeric/mutated G proteins making the calcium assays broadly applicable in GPCR research. Third, the price of instruments capable of measuring fluorescent-based calcium indicators has become significantly less making them obtainable even for academic groups. Here, we present a protocol for measuring changes in intracellular calcium levels in living mammalian cells based on the fluorescent calcium binding dye, fluo-4.
    OriginalsprogEngelsk
    TitelG protein-coupled receptors in drug discovery
    RedaktørerWayne R Leifert
    Vol/bind552
    UdgivelsesstedUnited States
    ForlagHumana Press
    Publikationsdato2009
    Sider269-278
    ISBN (Trykt)978-1-60327-316-9
    ISBN (Elektronisk)978-1-60327-317-6
    DOI
    StatusUdgivet - 2009
    NavnMethods in Molecular Biology
    Vol/bind552
    ISSN1064-3745

    Bibliografisk note

    Keywords: Intracellular calcium measurement, G protein-coupled receptors, probenecid, FLIPR, FlexStation, NOVO star.

    Chapter 19

    Emneord

    • Det tidligere Farmaceutiske Fakultet

    Citationsformater