Flow cytometric analysis of cerebrospinal fluid improves detection of leukaemic blasts in infants with acute lymphoblastic leukaemia

Maria Thastrup; Hanne Vibeke Marquart; Mette Levinsen; Signe Modvig; Jonas Abrahamsson; Birgitte Klug Albertsen; Britt Marie Frost; Arja Harila-Saari; Jouni Pesola; Aina Ulvmoen; Dorota Malgorzata Wojcik; Mervi Taskinen; Marianne Hoffmann; Birgitte Lausen; Kjeld Schmiegelow; on behalf of the Nordic Society of Paediatric Haematology, Oncology (NOPHO)

doi:10.1111/bjh.17769

Flow cytometric analysis of cerebrospinal fluid improves detection of leukaemic blasts in infants with acute lymphoblastic leukaemia

Maria Thastrup, Hanne Vibeke Marquart, Mette Levinsen, Signe Modvig, Jonas Abrahamsson, Birgitte Klug Albertsen, Britt Marie Frost, Arja Harila-Saari, Jouni Pesola, Aina Ulvmoen, Dorota Malgorzata Wojcik, Mervi Taskinen, Marianne Hoffmann, Birgitte Lausen, Kjeld Schmiegelow^*, on behalf of the Nordic Society of Paediatric Haematology, Oncology (NOPHO)

^*Corresponding author af dette arbejde

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › peer review

8 Citationer (Scopus)

Abstract

Infants with acute lymphoblastic leukaemia (ALL) have a high frequency of central nervous system (CNS) involvement. Flow cytometric analysis of cerebrospinal fluid (CSF) was recently demonstrated to be a sensitive method for detecting CNS involvement in childhood ALL. In the present study, CSF from 14 infants was collected at routine lumbar punctures and analysed by multicolour flow cytometry. At initial diagnosis, leukaemic blasts were detected in CSF by flow cytometry in 11 patients (78·6%) compared to seven patients (50%) by cytospin. Larger studies are needed to determine if CSF flow cytometry has prognostic value in infant ALL.

Originalsprog	Engelsk
Tidsskrift	British Journal of Haematology
Vol/bind	195
Udgave nummer	1
Sider (fra-til)	119-122
ISSN	0007-1048
DOI	https://doi.org/10.1111/bjh.17769
Status	Udgivet - 2021

Bibliografisk note

Funding Information:
We thank all Nordic paediatric oncology centres for providing clinical data and CSF samples for flow cytometric analysis. We are grateful to the staff at the Laboratory of Paediatric Oncology and the Department of Immunology, Rigshospitalet, for their help with sample analysis and data registration. The study was funded by the Danish Childhood Cancer Foundation, Nordic Cancer Union, Rigshospitalet, Swedish Childhood Cancer Foundation, Danish Cancer Research Foundation, Cancer Research Foundation of Elna and Jørgen Fagerholt Pedersen, Axel Muusfeldt’s Foundation, Anders Hasselbalch’s Fight Leukemia Foundation, Otto Christensen’s Foundation, Memorial Foundation of Merchant M. Brogaard and Wife, Research Foundation of Hasselbalch and Lykkegaard Andersen, Memorial Foundation of Eva and Henry Frænkel, Dagmar Marshall’s Foundation, King Christian the Tenth Foundation, Memorial Trust Foundation of Thora and Viggo Grove.

Publisher Copyright:
© 2021 British Society for Haematology and John Wiley & Sons Ltd

Adgang til dokumentet

10.1111/bjh.17769

Citationsformater

Thastrup, M., Marquart, H. V., Levinsen, M., Modvig, S., Abrahamsson, J., Albertsen, B. K., Frost, B. M., Harila-Saari, A., Pesola, J., Ulvmoen, A., Wojcik, D. M., Taskinen, M., Hoffmann, M., Lausen, B., Schmiegelow, K., & on behalf of the Nordic Society of Paediatric Haematology, Oncology (NOPHO) (2021). Flow cytometric analysis of cerebrospinal fluid improves detection of leukaemic blasts in infants with acute lymphoblastic leukaemia. British Journal of Haematology, 195(1), 119-122. https://doi.org/10.1111/bjh.17769

Thastrup, M, Marquart, HV, Levinsen, M, Modvig, S, Abrahamsson, J, Albertsen, BK, Frost, BM, Harila-Saari, A, Pesola, J, Ulvmoen, A, Wojcik, DM, Taskinen, M, Hoffmann, M, Lausen, B, Schmiegelow, K & on behalf of the Nordic Society of Paediatric Haematology, Oncology (NOPHO) 2021, 'Flow cytometric analysis of cerebrospinal fluid improves detection of leukaemic blasts in infants with acute lymphoblastic leukaemia', British Journal of Haematology, bind 195, nr. 1, s. 119-122. https://doi.org/10.1111/bjh.17769

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title = "Flow cytometric analysis of cerebrospinal fluid improves detection of leukaemic blasts in infants with acute lymphoblastic leukaemia",

abstract = "Infants with acute lymphoblastic leukaemia (ALL) have a high frequency of central nervous system (CNS) involvement. Flow cytometric analysis of cerebrospinal fluid (CSF) was recently demonstrated to be a sensitive method for detecting CNS involvement in childhood ALL. In the present study, CSF from 14 infants was collected at routine lumbar punctures and analysed by multicolour flow cytometry. At initial diagnosis, leukaemic blasts were detected in CSF by flow cytometry in 11 patients (78·6%) compared to seven patients (50%) by cytospin. Larger studies are needed to determine if CSF flow cytometry has prognostic value in infant ALL.",

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KW - ALL

KW - central nervous system

KW - cerebrospinal fluid

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KW - flow cytometry

KW - metastasis

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