Folding of heterologous proteins in bacterial cell factories: Cellular mechanisms and engineering strategies

Yixin Rong, Sheila Ingemann Jensen, Kresten Lindorff-Larsen, Alex Toftgaard Nielsen*

*Corresponding author af dette arbejde

Publikation: Bidrag til tidsskriftReviewForskningpeer review

12 Citationer (Scopus)

Abstract

The expression of correctly folded and functional heterologous proteins is important in many biotechnological production processes, whether it is enzymes, biopharmaceuticals or biosynthetic pathways for production of sustainable chemicals. For industrial applications, bacterial platform organisms, such as E. coli, are still broadly used due to the availability of tools and proven suitability at industrial scale. However, expression of heterologous proteins in these organisms can result in protein aggregation and low amounts of functional protein. This review provides an overview of the cellular mechanisms that can influence protein folding and expression, such as co-translational folding and assembly, chaperone binding, as well as protein quality control, across different model organisms. The knowledge of these mechanisms is then linked to different experimental methods that have been applied in order to improve functional heterologous protein folding, such as codon optimization, fusion tagging, chaperone co-production, as well as strain and protein engineering strategies.

OriginalsprogEngelsk
Artikelnummer108079
TidsskriftBiotechnology Advances
Vol/bind63
Antal sider21
ISSN0734-9750
DOI
StatusUdgivet - 2023

Bibliografisk note

Funding Information:
This work was funded by the Novo Nordisk Foundation within the framework of the Fermentation-based Biomanufacturing Initiative (FBM), grant number: NNF17SA0031362 . We further acknowledge funding from the Independent Research Foundation Denmark (Grant no. 7017-00321B ; SIJ), from the Novo Nordisk Foundation (Grant no. NNF20CC0035580 , Grant no. NNF15OC0016360 , and Grant no. NNF18OC0033950 ).

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