Fra-1 induces morphological transformation and increases in vitro invasiveness and motility of epithelioid adenocarcinoma cells

O. Kustikova; D. Kramerov; M. Grigorian; Vladimir Berezin; Elisabeth Marianne Bock; E. Lukanidin; E. Tulchinsky

Fra-1 induces morphological transformation and increases in vitro invasiveness and motility of epithelioid adenocarcinoma cells

O. Kustikova, D. Kramerov, M. Grigorian, Vladimir Berezin, Elisabeth Marianne Bock, E. Lukanidin, E. Tulchinsky

Institut for Neurovidenskab

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › peer review

176 Citationer (Scopus)

Abstract

Udgivelsesdato: 1998-Dec

Originalsprog	Engelsk
Tidsskrift	Molecular and Cellular Biology
Vol/bind	18
Udgave nummer	12
Sider (fra-til)	7095-7105
Antal sider	10
ISSN	0270-7306
Status	Udgivet - 1998

Bibliografisk note

Keywords: Adenocarcinoma; Animals; Cell Movement; DNA-Binding Proteins; Doxycycline; Gene Expression Regulation, Neoplastic; Genes, Reporter; Mice; Neoplasm Invasiveness; Neoplasm Metastasis; Neoplasm Proteins; Nuclear Proteins; Proto-Oncogene Proteins c-fos; Proto-Oncogene Proteins c-jun; RNA, Messenger; Transcription Factor AP-1; Transcription, Genetic; Transfection; Tumor Cells, Cultured

Citationsformater

@article{d444f8a074c911dbbee902004c4f4f50,

title = "Fra-1 induces morphological transformation and increases in vitro invasiveness and motility of epithelioid adenocarcinoma cells",

abstract = "Two cell lines originating from a common ancestral tumor, CSML0 and CSML100, were used as a model to study AP-1 transcription factors at different steps of tumor progression. CSML0 cells have an epithelial morphology; they express epithelial but not mesenchymal markers and are invasive neither in vitro nor in vivo. CSML100 possesses all characteristics of a highly progressive carcinoma. These cells do not form tight contacts, are highly invasive in vitro, and are metastatic in vivo. AP-1 activity was considerably higher in CSML100 cells than in CSML0 cells. There was a common predominant Jun component, namely, JunD, detected in both cell lines. We found that the enhanced level of AP-1 in CSML100 cells was due to high expression of Fra-1 and Fra-2 proteins, which were undetectable in CSML0 nuclear extracts. Analysis of the transcription of different AP-1 members in various cell lines derived from tumors of epithelial origin revealed a correlation of fra-1 expression with mesenchymal characteristics of carcinoma cells. Moreover, we show here for the first time that the expression of exogenous Fra-1 in epithelioid cells results in morphological changes that resemble fibroblastoid conversion. Cells acquire an elongated shape and become more motile and invasive in vitro. Morphological alterations were accompanied by transcriptional activation of certain genes whose expression is often induced at late stages of tumor progression. These data suggest a critical role of the Fra-1 protein in the development of epithelial tumors.",

author = "O. Kustikova and D. Kramerov and M. Grigorian and Vladimir Berezin and Bock, {Elisabeth Marianne} and E. Lukanidin and E. Tulchinsky",

note = "Keywords: Adenocarcinoma; Animals; Cell Movement; DNA-Binding Proteins; Doxycycline; Gene Expression Regulation, Neoplastic; Genes, Reporter; Mice; Neoplasm Invasiveness; Neoplasm Metastasis; Neoplasm Proteins; Nuclear Proteins; Proto-Oncogene Proteins c-fos; Proto-Oncogene Proteins c-jun; RNA, Messenger; Transcription Factor AP-1; Transcription, Genetic; Transfection; Tumor Cells, Cultured",

year = "1998",

language = "English",

volume = "18",

pages = "7095--7105",

journal = "Molecular and Cellular Biology",

issn = "0270-7306",

publisher = "American Society for Microbiology",

number = "12",

}

TY - JOUR

T1 - Fra-1 induces morphological transformation and increases in vitro invasiveness and motility of epithelioid adenocarcinoma cells

AU - Kustikova, O.

AU - Kramerov, D.

AU - Grigorian, M.

AU - Berezin, Vladimir

AU - Bock, Elisabeth Marianne

AU - Lukanidin, E.

AU - Tulchinsky, E.

N1 - Keywords: Adenocarcinoma; Animals; Cell Movement; DNA-Binding Proteins; Doxycycline; Gene Expression Regulation, Neoplastic; Genes, Reporter; Mice; Neoplasm Invasiveness; Neoplasm Metastasis; Neoplasm Proteins; Nuclear Proteins; Proto-Oncogene Proteins c-fos; Proto-Oncogene Proteins c-jun; RNA, Messenger; Transcription Factor AP-1; Transcription, Genetic; Transfection; Tumor Cells, Cultured

PY - 1998

Y1 - 1998

N2 - Two cell lines originating from a common ancestral tumor, CSML0 and CSML100, were used as a model to study AP-1 transcription factors at different steps of tumor progression. CSML0 cells have an epithelial morphology; they express epithelial but not mesenchymal markers and are invasive neither in vitro nor in vivo. CSML100 possesses all characteristics of a highly progressive carcinoma. These cells do not form tight contacts, are highly invasive in vitro, and are metastatic in vivo. AP-1 activity was considerably higher in CSML100 cells than in CSML0 cells. There was a common predominant Jun component, namely, JunD, detected in both cell lines. We found that the enhanced level of AP-1 in CSML100 cells was due to high expression of Fra-1 and Fra-2 proteins, which were undetectable in CSML0 nuclear extracts. Analysis of the transcription of different AP-1 members in various cell lines derived from tumors of epithelial origin revealed a correlation of fra-1 expression with mesenchymal characteristics of carcinoma cells. Moreover, we show here for the first time that the expression of exogenous Fra-1 in epithelioid cells results in morphological changes that resemble fibroblastoid conversion. Cells acquire an elongated shape and become more motile and invasive in vitro. Morphological alterations were accompanied by transcriptional activation of certain genes whose expression is often induced at late stages of tumor progression. These data suggest a critical role of the Fra-1 protein in the development of epithelial tumors.

AB - Two cell lines originating from a common ancestral tumor, CSML0 and CSML100, were used as a model to study AP-1 transcription factors at different steps of tumor progression. CSML0 cells have an epithelial morphology; they express epithelial but not mesenchymal markers and are invasive neither in vitro nor in vivo. CSML100 possesses all characteristics of a highly progressive carcinoma. These cells do not form tight contacts, are highly invasive in vitro, and are metastatic in vivo. AP-1 activity was considerably higher in CSML100 cells than in CSML0 cells. There was a common predominant Jun component, namely, JunD, detected in both cell lines. We found that the enhanced level of AP-1 in CSML100 cells was due to high expression of Fra-1 and Fra-2 proteins, which were undetectable in CSML0 nuclear extracts. Analysis of the transcription of different AP-1 members in various cell lines derived from tumors of epithelial origin revealed a correlation of fra-1 expression with mesenchymal characteristics of carcinoma cells. Moreover, we show here for the first time that the expression of exogenous Fra-1 in epithelioid cells results in morphological changes that resemble fibroblastoid conversion. Cells acquire an elongated shape and become more motile and invasive in vitro. Morphological alterations were accompanied by transcriptional activation of certain genes whose expression is often induced at late stages of tumor progression. These data suggest a critical role of the Fra-1 protein in the development of epithelial tumors.

M3 - Journal article

C2 - 9819396

SN - 0270-7306

VL - 18

SP - 7095

EP - 7105

JO - Molecular and Cellular Biology

JF - Molecular and Cellular Biology

IS - 12

ER -